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Strain Detail Sheet

The live colony for this line will soon be taken off the shelf. Once off the shelf, this line will only be available as cryopreserved germplasm or mice from cryo recovery. Place your orders online by 06/18/2025 to receive mice from the live colony.

Strain Name:
STOCK Slc4a4tm2.1Ges/IdwufMmjax
Stock Number:
072087-JAX
Citation ID:
RRID:MMRRC_072087-JAX
Other Names:
Floxed Slc4a4 (NBCe1)

Strain Information

Gene Details

Slc4a4tm2.1Ges
Name: solute carrier family 4 (anion exchanger), member 4; targeted mutation 2.1, Gary E Shull
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 5
Alteration at locus: Conditional
Slc4a4
Name: solute carrier family 4 (anion exchanger), member 4
Synonyms: NBC, NBC1
Type: Gene
Species: Mouse
Chromosome: 5
Alteration at locus: Conditional
NCBI: 54403
Homologene: 55776

Additional Resources
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Google
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Gene Ontology
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Mouse Phenome DB
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KOMP Phenotyping
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Genetic Alterations

Slc4a4-floxed mice carry loxP sites flanking exon 12 of the Slc4a4 gene. These mice may be useful for generating conditional mutants when studying pH homeostasis, bicarbonate absorption and secretion.

Genotype Determination
ES Cell Line
129Sv derived from 129S2/SvPas

Strain Description

Phenotype
The Slc4a4 (solute carrier family 4 (anion exchanger), member 4) gene, also known as Nbc1, codes for a protein involved with regulating bicarbonate secretion and absorption. Mutations in this gene may be associated with cardiac dysfunction, proximal renal tubular acidosis (RTA), ocular abnormalities, and familial hemiplegic migraines. These Slc4a4-floxed mice have lox sites flanking exon 12 of the Slc4a4 gene.

Homozygous mice are viable and fertile with no overt phenotype. When bred to mice that express tissue-specific Cre recombinase, resulting offspring will have exon 12 deleted, ablating all Slc4a4 transcripts in the cre-expressing tissues. When these floxed mice are bred to a Pax8-Cre line, resulting in deletion of NBC1 in kidneys, the mice develop proximal RTA with metabolic acidosis. These mice may be useful when studying the effects of NBC1 on intracellular pH levels.
Strain Development
The Slc4a4-floxed targeting vector, originally created by Dr. Gary Shull, was designed to insert loxP sites flanking exon 12 of the solute carrier family 4 (anion exchanger), member 4 (Slc4a4) gene. The construct also contained an FRT-flanked neomycin cassette immediately upstream of the second loxP site. The final targeting construct was electroporated into 129SvJ-derived embryonic stem (ES) cells and correctly targeted ES cells were injected into recipient blastocysts. Chimeric mice were bred to a FLP recombinase mouse line on a C57BL/6 background to remove the neomycin cassette. Dr. David Weiner obtained some Slc4a4-floxed mice with neomycin cassette removed and floxed exon 12 intact. The Weiner laboratory bred these floxed mice to B6.129P2(Cg)-Pax8tm1.1(cre)Mbu/J (JAX Stock No. 028196) for multiple generations. Mice provided to The Jackson Laboratory were wildtype for the Pax8-Cre allele. Upon arrival, sperm was cryopreserved. To establish a live colony, an aliquot of frozen sperm was used to fertilize C57BL/6J oocytes.
Suggested Control Mice
Cre-negative controls.

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.

Research Applications

  • Cardiovascular
  • Cell Biology
  • Internal/Organ

Strain Origin

Donor
Gary Shull, University of Cincinnati College of Medicine.
I. David Weiner, M.D., University of Florida, Department of Medicine.
Primary Reference

Vairamani K, Prasad V, Wang Y, Huang W, Chen Y, Medvedovic M, Lorenz JN, Shull GE. NBCe1 Na+-HCO3- cotransporter ablation causes reduced apoptosis following cardiac ischemia-reperfusion injury in vivo. World J Cardiol. 2018 Sep 26;10(9):97-109. doi: 10.4330/wjc.v10.i9.97. (Medline PMID: 30344957)

Colony and Husbandry Information

Special Considerations

none

Health Status Report

For more information about this colony's health status contact csmmrrc@jax.org

Appearance

Coat Color
plain
Eye
plain

Breeding

MMRRC Breeding System
Other or uncertain
Generation
N/A
Overall Breeding Performance
Excellent

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Variable
Recommended wean age
3 Weeks
Average Pups Weaned
Variable

Order Request Information

Availability Level

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Sev Sheets.

Conditions of Distribution

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Sev Sheets

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)

The live colony for this line will soon be taken off the shelf. Once off the shelf, this line will only be available as cryopreserved germplasm or mice from cryo recovery. Place your orders online by 06/18/2025 to receive mice from the live colony.

MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
072087-JAX-HET-F
072087-JAX-HET-M
072087-JAX-WT-F
072087-JAX-WT-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
Wild Type Female
Wild Type Male
 $229.00 / $229.00
Non-Profit / For-Profit		 Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.