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Strain Detail Sheet

Strain Name:
C57BL/6J-Adgrb1em1Blr/Mmnc
Stock Number:
072128-UNC
Citation ID:
RRID:MMRRC_072128-UNC
Other Names:
Bai1 mGL-CT-IRES-Cre

Strain Information

Gene Details

Adgrb1
Name: adhesion G protein-coupled receptor B1
Synonyms: B830018M07Rik, Bai1
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 107831
HGNC: HGNC:943
Homologene: 1287

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Enhanced Green Fluorescent Protein (EGFP) was inserted into the C terminal tail of the GPCR and IRES-Cre into the 3' UTR.
The EGFP modification allows for imaging for the gene at the protein level. The Cre modification allows expression mapping when crossed with Ai9 mouse. The Cre modification also allows a variety of other experimental techniques using Cre-dependent viral delivery of molecular tools.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    C57Bl/6J-693 derived from C57BL/6J

    Strain Description

    Phenotype
    None/Normal/Wild-type

    Conditional phenotype: no

    Strain Development
    Cas9 RNP was injected into zygotes with a homology donor to create the insertion. The mouse strain used to obtain the embryos was C57BL/6J (JAX #000664). The mouse strain used to implant the blastocysts was CD-1 (Charles River Laboratory). C57BL/6J zygotes were microinjected with 400 nM Cas9 protein, 20 ng/ul guide RNA and 50 ng/ul donor. In vitro transcribed, purified guide RNA was diluted in microinjection buffer (5 mM Tris–HCl pH 7.5, 0.1 mM EDTA), heated at 95°C for 3 min, and placed on ice prior to addition of Cas9 protein. The mixture was then incubated at 37°C for 5 min and placed on ice, after which the donor oligonucleotide was added. The final mixture was filtered through a 0.2 uM filter and held on ice prior to microinjection. Microinjected embryos were implanted in recipient pseudopregnant females. Resulting pups were screened by PCR and sequencing for the presence of the mutant allele. Independent male founders were mated to wild-type C57BL/6J females, and transmitted the mutant allele through the germline to offspring.

    The UNC Animal Models Core Facility is supported in part by P30 CA016086 Cancer Center Core Support Grant to the UNC Lineberger Comprehensive Cancer Center.
    Suggested Control Mice
    -

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Research Tools

    Strain Origin

    Donor
    Bryan Roth, M.D., UNC Chapel Hill.
    Primary Reference
    Not ready to publish

    Colony and Husbandry Information

    Special Considerations

    -

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Sib-mating
    Generation
    -
    Overall Breeding Performance
    Excellent

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 7 to 9 months 7 to 9 months
    Average litter size
    7 to 9
    Recommended wean age
    24 days
    Average Pups Weaned
    7 to 9

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.

    Conditions of Distribution

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    072128-UNC-RESUS Litter recovered from cryo-archive $3,242.00 / $5,382.00
    Non-Profit / For-Profit    		 Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.