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Strain Detail Sheet

Strain Name:
B6;129S6-As3mttm1Djth/Mmnc
Stock Number:
072148-UNC
Citation ID:
RRID:MMRRC_072148-UNC
Other Names:
As3mt KO

Strain Information

Gene Details

As3mttm1Djth
Name: arsenite methyltransferase; targeted mutation 1, David Thomas
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Knockout
As3mt
Name: arsenite methyltransferase
Synonyms: Cyt19, 2310045H08Rik
Type: Gene
Species: Mouse
Chromosome: 19
Alteration at locus: Knockout
NCBI: 57344
VEGA: 19
Homologene: 10754

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
A neo gene replaced exons 3 and 4 and possibly part of 5 by homologous recombination. The knock-out was confirmed by western blotting of liver lysates.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    129Sv

    Strain Description

    Phenotype

    Knock out of As3mt gene results in accumulation of arsenic in tissues, and a pattern of methylation that is different from Wild Type. As3mt KO mice excrete predominantly inorganic arsenic and some monomethyl Arsenic in urine while wild type excrete predominantly dimethylarsenic.

    MeSH Terms
    • Administration, Oral
    • Animals
    • Arsenates/administration & dosage
    • Arsenates/pharmacokinetics
    • Arsenates/urine
    • Arsenic Poisoning
    • Arsenicals/metabolism
    • Arsenicals/urine
    • Female
    • Genotype
    • Humans
    • Male
    • Methyltransferases/genetics
    • Methyltransferases/metabolism
    • Mice
    • Mice, Inbred C57BL
    • Mice, Knockout
    • Phenotype
    • Tissue Distribution
    Strain Development
    A 12 kb DNA fragment containing exons 1 through 7 of the mouse As3mt gene was identified by screening a lambda phage library made of genomic DNA from mouse embryonic stem cells (derived from 129S6 strain). A targeting construct was designed to delete exons 3 through 5 that encode for amino acids important for the methyltransferase activity. A 1.2 kb Hind3/Sac1 fragment and a 6.6 kb Eag1/Nsi1 fragment was assembled as 5′ and 3′ arms of homology flanking a positively selectable marker gene, pMC1neo, and the herpes simplex TK gene was inserted into the plasmid as a negatively selectable marker gene. The plasmid DNA was linearized and introduced the targeting vector DNA into ES cells (derived from 129S6) via electroporation. G418-resistant ES cell colonies were expanded and screened by PCR and Southern blot analyses for the correct modification. Chimeras made with two of the five correctly modified ES cells transmitted the modified gene. The mutation was initially maintained in 129S6 mice. The mutant As3mt gene was transferred onto a C57BL/6 background through a series of backcrosses and the C57BL/6 heterozygous for mutant As3mt were intercrossed to generate homozygous mutant As3mt mice.
    Suggested Control Mice
    C57Bl/6 (Charles River) Wild type

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.

    Research Applications

    • Cancer
    • Cardiovascular
    • Diabetes
    • Metabolism
    • Neurobiology
    • Obesity

    Strain Origin

    Donor
    David Thomas, Ph.D., EPA.
    Mirek Styblo, Ph.D., University of North Carolina.
    Primary Reference

    Drobna Z, Naranmandura H, Kubachka KM, Edwards BC, Herbin-Davis K, Styblo M, Le XC, Creed JT, Maeda N, Hughes MF, Thomas DJ. Disruption of the arsenic (+3 oxidation state) methyltransferase gene in the mouse alters the phenotype for methylation of arsenic and affects distribution and retention of orally administered arsenate. Chem Res Toxicol. 2009 Oct;22(10):1713-20. doi: 10.1021/tx900179r. (Medline PMID: 19691357)

    Colony and Husbandry Information

    Special Considerations

    Recommended diet is a semi-purified diet (e.g. AIN93 ) or other diets low in Arsenic

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Random intra-strain mating
    Generation
    Unknown
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 10 to 12 months 10 to 12 months
    Average litter size
    Variable
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    Variable

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    The donor or their institution limits the distribution to non-profit institutions only.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    072148-UNC-RESUS Litter recovered from cryo-archive $3,242.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.