Loading...

Strain Detail Sheet

Published: 11/04/2025
Revised: 11/04/2025

Strain Name:
C57BL/6J-Srltm1Flc/Mmnc
Stock Number:
075675-UNC
Citation ID:
RRID:MMRRC_075675-UNC
Other Names:
Srl-flox

Strain Information

Gene Details

Srl
Name: sarcalumenin
Synonyms: sarcalumenin, sar, 9830004M20Rik
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 106393
Homologene: 45500

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
The 5' end of the Srl gene (encompassing the promoter) and the 1st exon are flanked by loxP sites.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    Not applicable

    Strain Description

    Phenotype

    Srl flox mice are viable and are born in expected ratios.


    Conditional phenotype: When Srl is ablated in cardiomyocytes using a Tnnt2-Cre driver, it leads to a sex-biased cardiac phenotype. Male mice exhibit prolonged cardiac repolarization as a result, while female mice show normal characteristics compared to the control group.

    MeSH Terms
    • MicroRNAs/genetics
    • MicroRNAs/metabolism
    • Animals
    • Male
    • Female
    • Humans
    • Mice
    • Sex Characteristics
    • Myocytes, Cardiac/metabolism
    • Genes, X-Linked
    • X Chromosome/genetics
    • Mice, Knockout
    • Mice, Inbred C57BL
    Strain GQC Summary
    Gene Specific Genotyping:

    To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

    Background Genetic Quality:

    The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

    Suggested Control Mice
    Littermates of all relevant genotypes.

    Research Applications

    • Cardiovascular

    Strain Origin

    Donor
    Frank Conlon, Ph.D., University of North Carolina at Chapel Hill.
    Primary Reference

    Emerson JI, Shi W, Paredes-Larios J, Walker WG, Hutton JE, Cristea IM, Marzluff WF, Conlon FL. X-Chromosome-Linked miRNAs Regulate Sex Differences in Cardiac Physiology. Circ Res. 2024 Dec 30. doi: 10.1161/CIRCRESAHA.124.325447. Epub ahead of print. (Medline PMID: 39772608)

    Strain Development
    Cas9 guide RNAs were designed to insert loxP sites flanking the 5’ end of Srl, encompassing the promoter and 1st exon. Selected guide RNAs were cloned into a T7-tRNA-guide RNA construct (UNC Animal Models Core) for T7 in vitro transcription of tRNA-gRNA fusion molecules. The in vitro transcribed tRNA-gRNAs were purified and eluted in microinjection buffer. The guide RNAs selected for embryo microinjection were Srl-5g63T and Srl-i1g40T. The donor plasmid included an 861 bp 5’ homology arm, 5’ loxP site inserted 385 bp 5’ of Srl exon 1, 620 bp critical region encompassing Srl promoter, exon 1, and 156 bp of intron 1, 3’loxP and FRT sites, and 1048 bp 3’ homology arm. Recombinant eSpCas9 protein was expressed in E. coli and purified. C57BL/6J zygotes were microinjected with eSpCas9 protein, 600 nM each tRNA-gRNA and 20 ng/ul donor vector in microinjection buffer. Injected embryos were implanted in recipient pseudopregnant females. Resulting pups were screened via PCR for the presence of the correct loxP insertions. Multiple founders with the Srl floxed allele were identified. Two founders were selected for sequence confirmation of the 5’ and 3’ loxP sites and mating to wild-type C57BL/6J animals for germline transmission of the floxed allele.


    Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

    Colony and Husbandry Information

    Special Considerations

    N/A

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Sib-mating
    Generation
    20
    Overall Breeding Performance
    Excellent

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 7 to 9 months Greater than 12 months
    Average litter size
    7 to 9
    Recommended wean age
    3 Weeks
    Average Pups Weaned
    7 to 9

    Order Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    A Commercial License Agreement from the Submitter is required for for-profit entities to use this strain. For more information, please contact Carmen Melvin

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    075675-UNC-RESUS Litter recovered from cryo-archive $3,242.00 / $5,382.00
    Non-Profit / For-Profit    		 Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.