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Strain Name:
B6(D2)-Slc2a1Rgsc200/Mmucd
Stock Number:
075737-UCD
Citation ID:
RRID:MMRRC_075737-UCD
Other Names:
B6(D2)-Slc2a1Rgsc200/1Rbrc

Strain Information

Slc2a1Rgsc200
Name: solute carrier family 2 (facilitated glucose transporter), member 1; RIKEN Genomic Sciences Center (GSC), 200
Synonyms: M100200, Glut1Rgsc200
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 4
Alteration at locus: Chemically Induced
Slc2a1
Name: solute carrier family 2 (facilitated glucose transporter), member 1
Synonyms: Glut-1, Glut1, M100200, Rgsc200
Type: Gene
Species: Mouse
Chromosome: 4
Alteration at locus: Chemically Induced
NCBI: 20525
Homologene: 68520
Genetic Alterations
This mutation was identified in an ENU mutagenesis screen. Sequence analysis of the gene revealed a T-to-C mutation in exon 7. The mutation resulted in a substitution of serine with proline at residue 324 of the encoded protein. RT-qPCR analysis showed a significant increase of Slc2a1 mRNA expression in the forebrain of adult heterozygotes. Immunoblotting showed no significant alterations of SLC2A1 protein expression in the forebrain of adult heterozygotes relative to wild-type controls.
ES Cell Line
Not applicable
Phenotype
Heterozygous mutants exhibit immobility seizures, learning deficiencies, epileptic electroencephalogram patters, reduced body weight, and lower cerebrospinal fluid glucose levels.
Homozygous mutants exhibit embryonic lethality.

Male heterozygous mutants exhibit reproductive difficulties in natural mating. However, in vitro fertilization (IVF) experiments show that their sperm have normal fertility. Female Heterozygous Mutants mutants display fertility in natural mating with B6J males. However, while they can give birth, they exhibit difficulty nursing, and all pups die shortly after birth.
MeSH Terms
  • Animals
  • Avoidance Learning
  • Behavior, Animal
  • Body Weight
  • Brain/metabolism
  • Brain/pathology
  • Carbohydrate Metabolism, Inborn Errors/genetics
  • Carbohydrate Metabolism, Inborn Errors/metabolism
  • Carbohydrate Metabolism, Inborn Errors/physiopathology
  • Disease Models, Animal
  • Electroencephalography
  • Embryo Loss/genetics
  • Embryo Loss/pathology
  • Glucose/cerebrospinal fluid
  • Glucose/metabolism
  • Glucose Transporter Type 1/genetics
  • Glucose Transporter Type 1/metabolism
  • Heterozygote
  • Homozygote
  • Kinetics
  • Learning
  • Mice, Mutant Strains
  • Monosaccharide Transport Proteins/deficiency
  • Monosaccharide Transport Proteins/genetics
  • Monosaccharide Transport Proteins/metabolism
  • Motor Activity
  • Mutation, Missense/genetics
  • Seizures/genetics
  • Seizures/pathology
  • Seizures/physiopathology
  • Sleep/physiology
  • Transcription, Genetic
  • Wakefulness/physiology
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
  • Metabolism
  • Models for Human Disease
  • Neurobiology
Donor
Tamio Furuse, RIKEN BioResource Research Center (BRC).
Primary Reference

Furuse T, Mizuma H, Hirose Y, Kushida T, Yamada I, Miura I, Masuya H, Funato H, Yanagisawa M, Onoe H, Wakana S. A new mouse model of GLUT1 deficiency syndrome exhibits abnormal sleep-wake patterns and alterations of glucose kinetics in the brain. Dis Model Mech. 2019 Sep 12;12(9):dmm038828. doi: 10.1242/dmm.038828. (Medline PMID: 31399478)

Strain Development
C57BL/6J mice were purchased from a commercial supplier. C57BL/6 males were treated with ENU (150-250 mg/kg body weight) by intraperitoneal (i.p.) injection and crossed with D2 females. The progeny generated by this cross were designated as G1 mice. We collected baseline data from the G1 mice and performed phenotypic screening on the G1 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Backcross
Generation
18
Overall Breeding Performance
Undetermined
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes Male heterozygous mutants exhibit reproductive difficulties in natural mating. However, in vitro fertilization (IVF) experiments show that their sperm have normal fertility. Female Heterozygous Mutants mutants display fertility in natural mating with B6J males. However, while they can give birth, they exhibit difficulty nursing, and all pups die shortly after birth.
Age Reproductive Decline: Undetermined Undetermined
Average litter size
Variable
Recommended wean age
4 Weeks
Average Pups Weaned
Variable

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Technology Licensing Division, RIKEN Innovation Co., Ltd..

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Technology Licensing Division, RIKEN Innovation Co., Ltd.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
075737-UCD-RESUS Litter recovered from cryo-archive $7,366.14 / $7,727.39
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.