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Strain Detail Sheet

Published: 09/15/2025
Revised: 09/15/2025

Strain Name:
B6.129S6(Cg)-Tcof1tm1.1Zhng/RshiMmnc
Stock Number:
075771-UNC
Citation ID:
RRID:MMRRC_075771-UNC
Other Names:
Floxed Tcof1

Strain Information

Gene Details

Tcof1tm1.1Zhng
Name: treacle ribosome biogenesis factor 1; targeted mutation 1.1, Jing Zhang
Synonyms: Tcof1fl
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 18
Alteration at locus: Conditional
Tcof1
Name: treacle ribosome biogenesis factor 1
Synonyms: treacle
Type: Gene
Species: Mouse
Chromosome: 18
Alteration at locus: Conditional
NCBI: 21453
Homologene: 68049

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Exon 1 of the Tcof1 gene is flanked by loxP sites.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    HZ2.2 derived from 129S6/SvEvTac

    Strain Description

    Phenotype
    Mice have no notable phenotype in the absence of cre recombination.

    Conditional phenotype: No

    MeSH Terms
    • Animals
    • GTP Phosphohydrolases
    • Haploinsufficiency/genetics
    • Intracellular Signaling Peptides and Proteins/genetics
    • Leukemia/genetics
    • Membrane Proteins
    • Mice
    • Monomeric GTP-Binding Proteins/genetics
    • Mutation
    • Nuclear Proteins
    • Phosphoproteins/genetics
    • Precursor Cells, T-Lymphoid
    • Cell Nucleolus/genetics
    • Cell Cycle
    • Cell Nucleus Division
    • Cell Proliferation
    • RNA Polymerase I/genetics
    • RNA, Ribosomal/genetics
    • Child
    • Child Abuse/psychology
    • Child Development
    • Child, Preschool
    • Female
    • Humans
    • Learning Disabilities/psychology
    • Male
    • Mother-Child Relations
    • Psychomotor Performance
    Strain GQC Summary
    Gene Specific Genotyping:

    To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

    Background Genetic Quality:

    The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

    Suggested Control Mice
    Littermates of all relevant genotypes.

    Research Applications

    • Cancer
    • Cell Biology
    • Developmental Biology
    • Models for Human Disease

    Strain Origin

    Donor
    Rita Shiang, Ph.D., Virginia Commonwealth University.
    Primary Reference

    Wen Z, Finn R, Gao X, Li L, Hebert A, Ranheim EA, Zhou Y, Yun G, Roose JP, Coon JJ, Shiang R, Wen R, Yu M, Wang D, Zhang J. Tcof1 haploinsufficiency promotes early T cell precursor-like leukemia in NrasQ61R/+ mice. Leukemia. 2022 Apr;36(4):1167-1170. doi: 10.1038/s41375-022-01510-7. Epub 2022 Jan 26. (Medline PMID: 35082403)

    Dash S, Lamb MC, Lange JJ, McKinney MC, Tsuchiya D, Guo F, Zhao X, Corbin TJ, Kirkman M, Delventhal K, Moore EL, McKinney S, Shiang R, Trainor PA. rRNA transcription is integral to phase separation and maintenance of nucleolar structure. PLoS Genet. 2023 Aug 28;19(8):e1010854. doi: 10.1371/journal.pgen.1010854. (Medline PMID: 37639467)

    Strain Development
    To create the targeting vector PSN7, two fragments of the Tcof1 gene were PCR-amplified, cloned, and sequenced. A loxP site was inserted upstream of exon 1, and the modified fragments were combined in the pTKLNCDL vector to generate PSN7. Sanger sequencing confirmed the desired direction and correct junction sequences. The PSN7 construct was linearized and 20 µg of DNA was electroporated into 129/SvEv ES (HZ2.2) cells. Three correctly targeted cell lines were identified by PCR and Southern analysis. To generate the conditional allele, one of the correctly targeted cell lines (1A5) was transiently transfected with Cre recombinase. Two clones that contained a deletion of the neo/CD cassette were used to generate the conditional knockout mice. Non-surviving clones were expanded and genotyped for the conditional allele by PCR and Southern analysis. Cell lines were karyotyped prior to blastocyst injections. To analyze the mouse model on a B6/129 background, Wnt1-Cre transgenic mice (Swiss Webster) were crossed with C57BL/6 mice for at least 10 generations. Then hemizygous Wnt1-Cre mice were mated with homozygous conditional allele mice to generate conditional heterozygous knockout mice. Conditional heterozygous knockout males were then used to mate with homozygous floxed Tcof1 females to generate homozygous conditional knockout mice.


    Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

    Colony and Husbandry Information

    Special Considerations

    N/A

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    Black
    Eye
    Black

    Breeding

    MMRRC Breeding System
    Sib-mating
    Generation
    N10
    Overall Breeding Performance
    Excellent

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: 7 to 9 months 7 to 9 months
    Average litter size
    Variable
    Recommended wean age
    4 Weeks
    Average Pups Weaned
    Variable

    Order Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Magdalena Morgan.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Magdalena Morgan

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    075771-UNC-RESUS Litter recovered from cryo-archive $3,242.00 / $5,382.00
    Non-Profit / For-Profit    		 Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.