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Strain Name:
B6;129S4-Slc37a4tm1Jyc/Mmucd
Stock Number:
075774-UCD
Citation ID:
RRID:MMRRC_075774-UCD
Other Names:
G6pt knockout mouse

Strain Information

Slc37a4tm1Jyc
Name: solute carrier family 37 (glucose-6-phosphate transporter), member 4; targeted mutation 1, Janice Yang Chou
Synonyms: G6PT-/-, Glc-6-PT
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 9
Alteration at locus: Knockout
Slc37a4
Name: solute carrier family 37 (glucose-6-phosphate transporter), member 4
Synonyms: G6pt1, G6PT
Type: Gene
Species: Mouse
Chromosome: 9
Alteration at locus: Knockout
NCBI: 14385
VEGA: 9
HGNC: HGNC:4061
Homologene: 37482
Genetic Alterations
The coding region of exon 1 as well as a portion of intron 1 were replaced with a neo cassette inserted by homologous recombination. Protein was undetected in homozygous mutant mice by Western blot analysis of liver and kidney microsomal preparations.
ES Cell Line
J1
Phenotype
Homozygous G6pt (Slc37a4) knockout mice exhibit similar characteristics as patients with GSD-Ib (Glycogen Storage Disease Type Ib). Homozygous mice are G6Pase deficient which is characterized by growth retardation, hypoglycemia, hepatomegaly, nephromegaly, hyperlipidemia, hyperuricemia and lactic acidemia.
MeSH Terms
  • Animals
  • Antiporters/deficiency
  • Calcium/metabolism
  • Chemokines/metabolism
  • Chemotaxis, Leukocyte
  • Disease Models, Animal
  • Genes, Recessive
  • Genetic Variation
  • Glucose/metabolism
  • Glycogen Storage Disease Type I/etiology
  • Glycogen Storage Disease Type I/pathology
  • Homeostasis
  • Kidney/pathology
  • Kinetics
  • Liver/pathology
  • Mice
  • Mice, Knockout
  • Monosaccharide Transport Proteins/deficiency
  • Neutropenia/etiology
  • Neutropenia/physiopathology
  • Neutrophils/metabolism
  • Respiratory Burst
  • Restriction Mapping
  • Time Factors
Strain Development
The 129/SvJ mouse G6PT gene (Slc37a4), composed of nine exons spanning 6.4 kb, was isolated, mapped and sequenced. The gene was disrupted by replacing exon 1 and the flanking intron 1 with a neomycin (neo) cassette and inserted into a targeting vector containing a herpes simplex virus thymidine kinase (HSV-tk) for negative selection. The construct was electroporated into J1 ES cells (derived from 129S4/SvJae) and seven targeted clones were identified from 250 doubly resistant clones by Southern
analysis. Two independently targeted ES cell lines were injected into C57BL/6 blastocysts and implanted into pseudo-pregnant FVB/N foster mice to generate two lines of mice carrying the G6pt null mutation. Chimeric mice were mated with C57BL/6 mice and germline transmission of the G6pt null allele in F1 heterozygous mice was confirmed by PCR analysis of tail DNA. Heterozygous animals were interbred to generate homozygous F2 (G6pt −/−) mice.
Suggested Control Mice
age-matched G6pt+/+ and G6pt+/- mice, which have a wild-type phenotype, were used as controls.
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Hematology
  • Metabolism
  • Models for Human Disease
Donor
Janice Chou, Ph.D., NICHD/NIH.
Primary Reference

Chen LY, Shieh JJ, Lin B, Pan CJ, Gao JL, Murphy PM, Roe TF, Moses S, Ward JM, Lee EJ, Westphal H, Mansfield BC, Chou JY. Impaired glucose homeostasis, neutrophil trafficking and function in mice lacking the glucose-6-phosphate transporter. Hum Mol Genet. 2003 Oct 1;12(19):2547-58. doi: 10.1093/hmg/ddg263. Epub 2003 Aug 12. (Medline PMID: 12925567)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
The progenies are varied: either black or agouti
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
N/A
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Reduced Reduced
Homozygotes are fertile: Undetermined Undetermined
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 10 to 12 months
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Heather Gunas.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Heather Gunas

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
075774-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.