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Strain Detail Sheet

Strain Name:
B6;129S4-G6pc3tm1Jyc/Mmucd
Stock Number:
075775-UCD
Citation ID:
RRID:MMRRC_075775-UCD
Other Names:
G6pc3 knockout mouse

Strain Information

Gene Details

G6pc3tm1Jyc
Name: glucose 6 phosphatase, catalytic, 3; targeted mutation 1, Janice Y Chou
Synonyms: G6Pase-beta-, G6pc3-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Knockout
G6pc3
Name: glucose 6 phosphatase, catalytic, 3
Synonyms: 0710001K01Rik
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Knockout
NCBI: 68401
Homologene: 16304

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
A targeting vector was designed to replace exons 3-5 with a floxed neomycin resistance cassette. Western blot confirmed absence of protein in mutant brain, muscle and testis.
ES Cell Line
J1

Strain Description

Phenotype
G6Pase-β–deficient (G6pc3–/–) mice exhibit neutropenia; defects in neutrophil respiratory burst, chemotaxis, and calcium flux; and increased susceptibility to bacterial infection. Furthermore, G6pc3–/–mice with experimental peritonitis exhibit an increased expression of the glucose-regulated proteins upregulated during ER stress in their neutrophils and bone marrow. G6pc3–/– neutrophils exhibit an enhanced rate of apoptosis. G6pc3–/– mice that are unable to hydrolyze endoluminal G6P to glucose, manifest myeloid dysfunctions mimicking G6PT deficiency.
MeSH Terms
  • Animals
  • Bacterial Infections/enzymology
  • Bacterial Infections/genetics
  • Bacterial Infections/immunology
  • Blood Glucose/analysis
  • Disease Models, Animal
  • Genetic Predisposition to Disease
  • Glucose-6-Phosphatase/analysis
  • Glucose-6-Phosphatase/genetics
  • Glucose-6-Phosphatase/metabolism
  • Glucose-6-Phosphate/metabolism
  • Hematopoiesis/genetics
  • Homeostasis
  • Mice
  • Neutropenia/enzymology
  • Neutropenia/genetics
  • Neutrophils/enzymology
  • Neutrophils/immunology
  • Peritonitis/enzymology
  • Peritonitis/genetics
  • Peritonitis/microbiology
  • Protein Subunits/analysis
  • Protein Subunits/genetics
  • Protein Subunits/metabolism
Strain Development
The G6pc3 gene is a single-copy gene consisting of 6 exons. The targeting vector was designed to disrupt exons 3 to 5 using a neomycin cassette and contains a herpes simplex virus thymidine kinase for negative selection. The targeting vector was verified by restriction endonuclease mapping and DNA sequencing. The DNA construct was introduced into J1 ES cells (derived from 129S4/SvJae) by electroporation, the cells with the homologous recombination were selected with G418 and gancyclovir, and the disrupted G6pc3 gene was confirmed in these clones by Southern blotting. Two probes, one corresponding to a G6pc3–specific 5' region (500 bp) lying 1 kb upstream of the 5' BamHI site and the other to a G6pc3–specific 3' region (500 bp) lying 1.5 kb downstream of the 3' BamHI site, were used to confirm the mutation. In order to create the knockout mouse strains, 2 independently G6pc3–targeted ES cell clones were injected into C57BL/6 blastocysts and implanted into the uterine horns of pseudopregnant FVB/N foster mice. Two lines of mice carrying the G6pc3–null mutation were selected. Chimeric mice were mated with C57BL/6 mice, and germline transmission of the G6pc3–null allele in F1 heterozygous mice was confirmed by PCR. Heterozygous animals were interbred to generate homozygous F2 mice, which were genotyped by PCR.
Suggested Control Mice
age-matched G6pc3+/+ and G6pc3+/- mice, which have a wild-type phenotype, may be used as controls.

MMRRC Genetic QC

MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.

Research Applications

  • Hematology
  • Models for Human Disease

Strain Origin

Donor
Janice Chou, Ph.D., NICHD/NIH.
Primary Reference

Cheung YY, Kim SY, Yiu WH, Pan CJ, Jun HS, Ruef RA, Lee EJ, Westphal H, Mansfield BC, Chou JY. Impaired neutrophil activity and increased susceptibility to bacterial infection in mice lacking glucose-6-phosphatase-beta. J Clin Invest. 2007 Mar;117(3):784-93. doi: 10.1172/JCI30443. Epub 2007 Feb 22. (Medline PMID: 17318259)

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat Color
The coat color is either black or agouti.
Eye
Black

Breeding

MMRRC Breeding System
Sib-mating
Generation
N/A
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Reduced
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 7 to 9 months
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
075775-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / $7,650.23
Non-Profit / For-Profit		 Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.