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Strain Name:
C57BL6/N-Smpd3em1Rstot/Mmmh
Stock Number:
075818-MU
Citation ID:
RRID:MMRRC_075818-MU
Other Names:
Smpd3em1Rstot

Strain Information

QUALITY CONTROL OF C57BL6/N-Smpd3em1Rstot/Mmmh  |  University of Missouri
Last Updated:
Strain Data and Information Information by Submitter Assessed by MMRRC1,2
Published Provided
Allele-specific genotype3n.d.
Genetic backgroundn.d.
Viability of genotypes available for distributionn.d.
Specific Pathogen- Status YES
Recoverability of cryopreserved sperm/embryos4 n.d.
Gene or allele sequence3 n.d.
Gene or allele expression3n.d.
Gene or allele function3n.d.
Observable and/or measurable phenotypesn.d.
Fertility of genotypes available for distribution n.d.
Fecundity/breeding performance n.d.

1 When indicated as "assessed by MMRRC" ("YES"), please note that the information presented on this strain is to the best of our knowledge correct and up-to-date at the the MMRRC Distribution Center assigned to maintain and distribute this strain. This information is subject to change due to breeding, maintenance, and other actions. Please direct any questions on the information presented in this table to the MMRRC Distribution Center.

2 If an assessment has not been performed by the MMRRC (as indicated by "NO"), investigators may request specific testing for a fee. Requests should be submitted directly to the MMRRC Distribution Center assigned to the management, archiving, and distribution of the strain. A full listing of available testing and analytical services is available at https://www.mmrrc.org/about/services.php.

3 This information may or may not apply to each individual engineered allele (e.g., Cre, FlpO) present in the strain.

4 Recovery refers to thawing, in vitro fertilization (IVF), and/or embryo culture leading to live offspring.

Smpd3em1Rstot
Name: sphingomyelin phosphodiesterase 3, neutral; endonuclease-mediated mutation 1, Rolf Stottmann
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 8
Alteration at locus: CRISPR
Smpd3
Name: sphingomyelin phosphodiesterase 3, neutral
Synonyms: neutral sphingomyelinase II, nSMase2, 4631433G07Rik, fro, Nsm2
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 58994
Homologene: 10260
Genetic Alterations

CRISPR/Cas9 genome editing in 1 cell-stage C57BL/6N zygotes was used to generate a 9,514 base-pair deletion corresponding to exons 3-7 of the Smpd3 gene.

ES Cell Line
Not applicable
Phenotype
Deletion of Smpd3 results in perinatal lethality.
Homozygous Smpd3 null embryos exhibit severe skeletal abnormalities, including shortened mandibles and limb bones (radius, humerus and scapula) at E18.5. Embryos do not exhibit any obvious cortical abnormalities at E14.5 or E16.5.
MeSH Terms
  • Animals
  • Humans
  • Cilia/metabolism
  • Ceramides/metabolism
  • Sphingolipids/metabolism
  • Mice
  • Brain/metabolism
  • Brain/embryology
  • Sphingomyelin Phosphodiesterase/metabolism
  • Sphingomyelin Phosphodiesterase/genetics
  • Induced Pluripotent Stem Cells/metabolism
  • Purkinje Cells/metabolism
  • Neural Stem Cells/metabolism
  • Neural Stem Cells/cytology
  • Cerebellum/metabolism
  • Cerebellum/growth & development
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
  • Brain embryology
  • Brain metabolism
  • Ceramides
  • Cerebellum growth & development
  • Cerebellum metabolism
  • Cilia metabolism
  • Induced Pluripotent Stem Cells metabolism
  • Neural Stem Cells
  • Neural Stem Cells
  • Purkinje Cells
  • Sphingolipids
  • Sphingomyelin Phosphodiesterase
  • Developmental Biology
Submitter
Rolf Stottmann, Ph.D., Nationwide Children's Hospital.
Primary Reference

Inskeep KA, Crase B, Dayarathna T, Stottmann RW. SMPD4-mediated sphingolipid metabolism regulates brain and primary cilia development. Development. 2024 Nov 15;151(22):dev202645. doi: 10.1242/dev.202645. Epub 2024 Nov 15. (Medline PMID: 39470011)

Strain Development
C57BL6/N zygotes were injected with 200 ng/µl CAS9 protein and 100 ng/µl Smpd3-specific sgRNA (TGGCCAGAGCAGGCTGCACGCGG, CAGGTCCTAAAGCAGCAGTCAGG), followed by implantation into pseudo-pregnant female CD-1 mice. The resulting pups were weaned and used for PCR screening and mating.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Uncertain
Eye
Uncertain
MMRRC Breeding System
Other or uncertain
Generation
N/A
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: N/A N/A
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
4 Weeks
Average Pups Weaned
7 to 9

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

A Commercial License Agreement from the Submitter is required for for-profit entities to use this strain. For more information, please contact Andrew Corris.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Submitter is required for for-profit entities to use this strain. For more information, please contact Andrew Corris

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
075818-MU-SPERM Cryo-preserved spermatozoa $546.25 / $1,021.25
Non-Profit / For-Profit
Aliquot Approximate quantity3
075818-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the submitter as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.