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Strain Detail Sheet

Published: 11/11/2025
Revised: 11/17/2025

Strain Name:
B6J.B6N(129S4)-Zc3h14tm1d(KOMP)Wtsi/KraeMmucd
Stock Number:
075870-UCD
Citation ID:
RRID:MMRRC_075870-UCD
Other Names:
Msut2 KO

Strain Information

Strain Quality Control

QUALITY CONTROL OF B6J.B6N(129S4)-Zc3h14tm1d(KOMP)Wtsi/KraeMmucd  |  University of California at Davis
Last Updated:
Strain Data and Information Information by Submitter Assessed by MMRRC1,2
Published Provided
Allele-specific genotype3 n.d.
Genetic backgroundn.d.
Viability of genotypes available for distributionn.d.
Specific Pathogen- Status n.d.
Recoverability of cryopreserved sperm/embryos4 n.d.
Gene or allele sequence3 n.d.
Gene or allele expression3n.d.
Gene or allele function3n.d.
Observable and/or measurable phenotypesn.d.
Fertility of genotypes available for distribution n.d.
Fecundity/breeding performance n.d.

1 When indicated as "assessed by MMRRC" ("YES"), please note that the information presented on this strain is to the best of our knowledge correct and up-to-date at the the MMRRC Distribution Center assigned to maintain and distribute this strain. This information is subject to change due to breeding, maintenance, and other actions. Please direct any questions on the information presented in this table to the MMRRC Distribution Center.

2 If an assessment has not been performed by the MMRRC (as indicated by "NO"), investigators may request specific testing for a fee. Requests should be submitted directly to the MMRRC Distribution Center assigned to the management, archiving, and distribution of the strain. A full listing of available testing and analytical services is available at https://www.mmrrc.org/about/services.php.

3 This information may or may not apply to each individual engineered allele (e.g., Cre, FlpO) present in the strain.

4 Recovery refers to thawing, in vitro fertilization (IVF), and/or embryo culture leading to live offspring.

Gene Details

Zc3h14tm1d(KOMP)Wtsi
Name: zinc finger CCCH type containing 14; targeted mutation 1d, Wellcome Trust Sanger Institute
Type: Allele
Species: Mus musculus
Chromosome: 12
Alteration at locus: Knockout
Zc3h14
Name: zinc finger CCCH type containing 14
Synonyms: 1700016A15Rik, 1010001P15Rik, 2700069A02Rik
Type: Gene
Species: Mouse
Chromosome: 12
NCBI: 75553
VEGA: 12
Homologene: 32605

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
Mice harboring a floxed allele of Zc3h14 were bred to a cre-expressing strain to remove the inserted cassette and exon 13 of the Zc3h14 gene, resulting in a knockout allele.
ES Cell Line
JM8A3.N1

Strain Description

Phenotype
Homozygous mice are phenotypically normal. Other groups have reported that Zc3h14 (Msut2) knockout mice have cognitive defects and enlarged brain ventricles, these phenotypes are not observed in these mice, indicating that this strain is unique.

Conditional phenotype: When in combination with a tau transgene that causes neurodegeneration, transgenic PS19 mice overexpressing tau and lacking the Zc3h14 (Msut2) gene exhibited decreased learning and memory deficits, reduced neurodegeneration, and reduced accumulation of pathological tau compared to PS19 tau transgenic mice expressing Zc3h14 (Msut2).
MeSH Terms
  • Alzheimer Disease/genetics
  • Alzheimer Disease/metabolism
  • Animals
  • Brain/metabolism
  • Carrier Proteins/genetics
  • Carrier Proteins/metabolism
  • Disease Models, Animal
  • Male
  • Mice
  • Mice, Transgenic
  • Poly(A)-Binding Protein I/genetics
  • Poly(A)-Binding Protein I/metabolism
  • Poly(A)-Binding Proteins/genetics
  • Poly(A)-Binding Proteins/metabolism
  • tau Proteins/genetics
  • tau Proteins/metabolism
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Models for Human Disease

Strain Origin

Submitter
Brian Kraemer, Ph.D., VAPSHCS.
Primary Reference

Wheeler JM, McMillan P, Strovas TJ, Liachko NF, Amlie-Wolf A, Kow RL, Klein RL, Szot P, Robinson L, Guthrie C, Saxton A, Kanaan NM, Raskind M, Peskind E, Trojanowski JQ, Lee VMY, Wang LS, Keene CD, Bird T, Schellenberg GD, Kraemer B. Activity of the poly(A) binding protein MSUT2 determines susceptibility to pathological tau in the mammalian brain. Sci Transl Med. 2019 Dec 18;11(523):eaao6545. doi: 10.1126/scitranslmed.aao6545. (Medline PMID: 31852801)

Strain Development
B6-Zc3h14tm1a(KOMP)Wtsi ES cells were used for microinjection and the chimera were mated to C57BL/6J for germline derived F1 mice. F1 mice were mated to C57BL/6J for colony expansion. Heterozygous offspring were mated to produce homozygotes, and the strain was subsequently maintained as a homozygous line. B6-Zc3h14tm1a animals were crossed with a Flp expressing strain (129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J), resulting in a floxed conditional allele. Mice with the conditional allele were then crossed with a cre-expressing strain (B6.129S4-Meox2tm1(cre)Sor/J) to remove exon 13 of the Zc3h14 gene as well as all inserted transgene sequences. This strain was then backcrossed to C57BL/6J for at least eight generations.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

For more information about this colony's health status contact mmrrc@ucdavis.edu

Appearance

Coat Color
Black
Eye
Black

Breeding

Breeding Scheme(s)
This strain was backcrossed to C57BL/6J for 8 generations and then intercrossed (brother x sister) for 6 generations.
Generation
N8
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 7 to 9 months 7 to 9 months
Average litter size
4 to 6
Recommended wean age
4 Weeks
Average Pups Weaned
4 to 6

Order Information

Availability Level

The availability level for this product has not been determined.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.