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Strain Detail Sheet

Published: 04/27/2026
Revised: 04/27/2026

Strain Name:
B6N.B6J-Sox10tm1.1Sout/Mmmh
Stock Number:
075977-MU
Citation ID:
RRID:MMRRC_075977-MU
Other Names:
B6.Sox10

Strain Information

Strain Quality Control

QUALITY CONTROL OF B6N.B6J-Sox10tm1.1Sout/Mmmh  |  University of Missouri
Last Updated:
Strain Data and Information Information by Submitter Assessed by MMRRC1,2
Published Provided
Allele-specific genotype3n.d.
Genetic backgroundn.d.
Viability of genotypes available for distribution n.d.
Specific Pathogen- Status n.d.
Recoverability of cryopreserved sperm/embryos4 n.d.
Gene or allele sequence3 n.d.
Gene or allele expression3n.d.
Gene or allele function3n.d.
Observable and/or measurable phenotypesn.d.
Fertility of genotypes available for distribution n.d.
Fecundity/breeding performance n.d.

1 When indicated as "assessed by MMRRC" ("YES"), please note that the information presented on this strain is to the best of our knowledge correct and up-to-date at the the MMRRC Distribution Center assigned to maintain and distribute this strain. This information is subject to change due to breeding, maintenance, and other actions. Please direct any questions on the information presented in this table to the MMRRC Distribution Center.

2 If an assessment has not been performed by the MMRRC (as indicated by "NO"), investigators may request specific testing for a fee. Requests should be submitted directly to the MMRRC Distribution Center assigned to the management, archiving, and distribution of the strain. A full listing of available testing and analytical services is available at https://www.mmrrc.org/about/services.php.

3 This information may or may not apply to each individual engineered allele (e.g., Cre, FlpO) present in the strain.

4 Recovery refers to thawing, in vitro fertilization (IVF), and/or embryo culture leading to live offspring.

Gene Details

Sox10tm1.1Sout
Name: SRY (sex determining region Y)-box 10; targeted mutation 1.1, E Southard-Smith
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 15
Alteration at locus: Knock-In
Sox10
Name: SRY (sex determining region Y)-box 10
Synonyms: Sox21, gt
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 20665
VEGA: 15
Homologene: 5055

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
A conditional by inversion (COIN) intron (as described in PMID: 23918385), consisting lox66_SA-Egpf-polyA_lox71, was inserted in the antisense orientation into intron 3 via homologous recombination, splitting intron 3 into 2 new exons (94 and 2,258 bp in length). The two lox sites are in reverse complementation orientation, thus cre-mediated recombination will irreversibly invert the inserted sequence. In addition, an FRT flanked drug selection cassette (hygromycin- or neomycin-delta-thymidine kinase cassette) was inserted after the COIN intron.

Mice were bred to Flp deleter mice (B6.129S4.Gt(ROSA)26Sortm1(FLP1)/Dym/RainJ) to excise the Neomycin delta thymidine kinase cassette.

Breeding with Cre-expressing line results in flipping of the splice acceptor, deletion of the normal Sox10 transcriptional activation domain and expression of a JRed reporter
ES Cell Line
JM8.F6 derived from C57BL/6N

Strain Description

Phenotype
  • Heterozygous mice:
    • Do not exhibit an overt phenotype
    • Prior to Cre-mediated recombination, are indistinguishable from wild-type animals
  • Homozygous mice:
    • Are not postnatal viable
    • Prior to Cre-mediated recombination, pups exhibit ventral belly spotting and aganglionic megacolo that cause premature death as a result of intestinal pseudo-obstruction

Conditional Phenotype: When crossed with a neural crest specific Cre driver, heterozygous offspring exhibit hypopigmentation and gut motility defects. It is recommended that the line should be maintained as heterozygotes.

MeSH Terms
  • Alleles
  • DNA Nucleotidyltransferases/metabolism
  • Gene Silencing
  • Genetic Engineering/methods
  • Mutagenesis, Insertional/methods
  • Sequence Inversion/genetics
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Developmental Biology
  • Models for Human Disease
  • Neurobiology

Strain Origin

Submitter
Michelle Southard-smith, Vanderbilt University.
Primary Reference

Additional References

Economides AN, Frendewey D, Yang P, Dominguez MG, Dore AT, Lobov IB, Persaud T, Rojas J, McClain J, Lengyel P, Droguett G, Chernomorsky R, Stevens S, Auerbach W, Dechiara TM, Pouyemirou W, Cruz JM Jr, Feeley K, Mellis IA, Yasenchack J, Hatsell SJ, Xie L, Latres E, Huang L, Zhang Y, Pefanis E, Skokos D, Deckelbaum RA, Croll SD, Davis S, Valenzuela DM, Gale NW, Murphy AJ, Yancopoulos GD. Conditionals by inversion provide a universal method for the generation of conditional alleles. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):E3179-88. doi: 10.1073/pnas.1217812110. Epub 2013 Aug 5. (Medline PMID: 23918385)

Strain Development
The Sox10-COIN construct was generated by incorporating a Neo drug selection cassette driven by the human UB(c) promoter flanked by FRT sites as well as an inversion cassette containing a splice acceptor site linked to the red fluorescent protein JRed followed by a rabbit B-globin polyadenylation sequence. This 'COIN' cassette was introduced into a Sox10 BAC clone RP23 28O11 by in vitro homologous recombination in E. coli that resulted in targeted integration downstream of Sox10 exon 4. The targeting vector was linearized and electroporated into JM8.F6 ES cells derived from C57BL/6N (Pettitt et al., 2009) which were subsequently subjected to G418 selection. 480 clones were picked into individual wells of 96-well plates and were then screened by PCR for integration of the JRed reporter. PCR-positive clones were subsequently confirmed by Southern blotting. ES cell clones were introduced into C57BL/6J-TyrC-2J/J blastocysts. Chimeras were bred for transmission of black offspring and subsequent progeny were bred with Flp deleter mice (B6.129S4.Gt(ROSA)26Sortm1(FLP1)/Dym/RainJ) to excise the Neomycin delta thymidine kinase cassette. Progeny lacking the Neo cassette were bred with C57BL/6J mice to make Sox10COIN lines congenic on this inbred strain background.

Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Special Considerations

N/A

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Black
Eye
Black

Breeding

MMRRC Breeding System
Backcross
Generation
>20
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
4 to 6
Recommended wean age
3 Weeks
Average Pups Weaned
4 to 6

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The submitter or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
075977-MU-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
075977-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.