Strain Detail Sheet

Strain Name    :

C3;B6-l11Jus3/Mmucd

Stock Number :

000076-UCD

Other Names   :

Lethal 3, l11Jus3, C3;B6-l11Jus3/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: l11Jus3
Name: lethal, Chr 11, Justice 3
Chromosome: 11; 50.5 cM
Alteration at locus: Chemically Induced
Gene Symbol: l11Jus3
Name: lethal, Chr 11, Justice 3
Chromosome: 11
Alteration at locus: Chemically Induced

Genetic Alterations:
Offspring of an ENU-induced male.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Lethal at 6.5dpc, failure to form epiblast, pre-gastrulation defect


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
l11Jus3/l11Jus3
        Background Not Specified
  • mortality/aging
    • complete embryonic lethality before somite formation (MGI Ref ID J:109155)
      • animals die between 5.5 and 8.5 dpc
  • embryogenesis phenotype
    • abnormal developmental patterning (MGI Ref ID J:85113)
      • failure to gastrulate (MGI Ref ID J:85113)
    • abnormal embryonic tissue morphology (MGI Ref ID J:109155)
      • homozygotes do not form embryonic cavities, suggesting a failure of axis organization and specification
      • abnormal egg cylinder morphology (MGI Ref ID J:109155)
    • abnormal visceral endoderm morphology (MGI Ref ID J:85113)
      • abnormal visceral endoderm cells
    • abnormal visceral endoderm morphology (MGI Ref ID J:109155)
      • homozygotes lack differentiated visceral endoderm
      • mutant visceral endoderm cells are morphologically abnormal
      • visceral endoderm continues to grow with the egg cylinder, causing cells to appear lacy

Strain of Origin: C57BL/6

Strain genetic background: C3H

Strain Development: For strain development, view: http://www.mouse-genome.bcm.tmc.edu/ENU/ENUexperiment.asp#Balancer

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Birth Defects

Strain Origin

Donor: Monica J. Justice, Ph.D., Baylor College of Medicine

Primary Reference:

Kile BT, Hentges KE, Clark AT, Nakamura H, Salinger AP, Liu B, Box N, Stockton DW, Johnson RL, Behringer RR, Bradley A, Justice MJ. Functional genetic analysis of mouse chromosome 11. Nature. 2003 Sep 4;425(6953):81-6. (Medline PMID: 12955145)

Colony and Husbandry Information

Special Considerations

In order to determine the genotype of MMRRC:000076, a Lethal strain, it must be used in conjunction with MMRRC:009973. MMRRC:009973 is a combination of MMRRC:000055 and The Jackson Laboratory strain 000268; this combination results in a curly coated, yellow ear and yellow tail mouse which is a reporter for chromosome 11 mutations in the Lethal strains.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color: Agouti

Other:


Breeding

MMRRC Breeding System: The MMRRC has always maintained this strain in a cryopreserved format. Information in the "Breeding" and "Reproductive statistics" sections has been provided by the Donating Investigator

Breeding Scheme(s):

Generation: Not applicable

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: Unknown Unknown

Average litter size: 4

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000076-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
000076-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
000076-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

This site requires JavaScript. Tested for Firefox 3.5+, Microsoft Internet Explorer 7+, Google Chrome.

Generated: 04/23/2014