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Strain Name:
C57BL/6J-Vclem1Glr/Mmmh
Stock Number:
076082-MU
Citation ID:
RRID:MMRRC_076082-MU
Other Names:
Vcl Y822F

Strain Information

QUALITY CONTROL OF C57BL/6J-Vclem1Glr/Mmmh  |  University of Missouri
Last Updated:
Strain Data and Information Information by Submitter Assessed by MMRRC1,2
Published Provided
Allele-specific genotype3 n.d.
Genetic background n.d.
Viability of genotypes available for distribution n.d.
Specific Pathogen- Status n.d.
Recoverability of cryopreserved sperm/embryos4 n.d.
Gene or allele sequence3 n.d.
Gene or allele expression3 n.d.
Gene or allele function3 n.d.
Observable and/or measurable phenotypes n.d.
Fertility of genotypes available for distribution n.d.
Fecundity/breeding performance n.d.

1 When indicated as verified ("YES"), then please note that information presented is to the best of our knowledge correct and up-to-date at the time of verification at the MMRRC Distribution Center; however, this information is subject to change due to breeding, maintenance, and other actions on the mouse strain at the MMRRC Distribution Center; direct any questions on this table to the MMRRC Distribution Center for this mouse stain.

2 If verification has not been performed (as indicated by "NO"), investigators may request specific verification testing for a fee. Requests should be submitted directly to the MMRRC Distribution Center assigned to the management, archiving, and distribution of the strain. A full listing of available testing and analytical services is available at https://www.mmrrc.org/about/services.php.

3 This information may or may not apply to each individual engineered allele (e.g., Cre, FlpO) present in the strain.

4 Recovery refers to thawing, in vitro fertilization (IVF), and/or embryo culture leading to live offspring.

Vcl
Name: vinculin
Synonyms: metavinculin
Type: Gene
Species: Mouse
Chromosome: 14
NCBI: 22330
VEGA: 14
Homologene: 7594
Genetic Alterations
CRISPR/Cas9 mediated genome editing introduced a tyrosine-to-phenylalanine substitution at amino acid 822 (Y822F) in the Vcl locus. This single amino acid change abolishes phosphorylation at residue Y822 without affecting overall Vcl protein expression or stability, thereby creating a non-phosphorylatable Vcl variant.
ES Cell Line
Not Applicable
Phenotype
A non-phosphorylatable Vcl variant (Vcl Y822F) was developed to investigate the role of Vcl Y822 phosphorylation in heart development. Vcl Y822F mice are viable, fertile, and show no gross morphological defects. However, Vcl Y822F mutant mice exhibit age-dependent cardiac defects including a reduction in left ventricuar function at 28 weeks of age which was not observed in younger mice (10 weeks). This suggests that adhesion defects caused by the loss of phosphorylatable Y822 (pY822) manifest as functional cardiac impairment with age.

During early postnatal development, Vcl Y822F neonatal hearts (P7) exhibit increased α5 integrin and fibronectin expression compared to wild type controls, suggesting enhanced cell-extracellular matrix (ECM) adhesion.

The Vcl Y822F mutant mouse described here is a constitutive knockout, preventing Y822 phosphorylation in all tissues. Thus, the Y822F mutation could affect Vcl function in other cardiac or vascular cell types (e.g., cardiac fibroblasts, endothelial cells).
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Developmental Biology
Donor
Glenn Radice, Ph.D., Rhode Island Hospital/Brown University.
Primary Reference

Li X, Wortelboer R, Song Y, Balasubramanian S, McLain C, Hernandez Manriquez A, Suh JD, Hoffman BD, Kwiatkowski AV, Radice GL. Vinculin Y822 phosphorylation regulates adhesion remodeling during cardiomyocyte maturation. J Cell Sci. 2025 Dec 15;138(24):jcs263984. doi: 10.1242/jcs.263984. Epub 2025 Dec 18. (Medline PMID: 41231303)

Strain Development
CRISPR-targeting reagents were injected into the cytoplasm of the C57BL/6J mouse zygotes. The injection mix contained SpCas9 nuclease, the annealed guide RNA , the single-stranded DNA oligonucleotide carrying the targeted mutation VclY822F (TAT >TtT), and a silent mutation to disrupt the targeting site PAM domain. A gRNA sequence close to the mutation site and with minimum potential off-target cleavage activity was selected. Mosaic VclY822F/+ founder mice were bred with wild-type C57BL/6J mice for four generations to diminish potential off-target mutations. Two independent VclY822F/+ founder lines were intercrossed to generate homozygous VclY822F/Y822F mice. Mice were genotyped by PCR.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
Eye
Black
MMRRC Breeding System
Random intra-strain mating
Generation
N/A
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Margarita Shabashevich.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Submitter is required for for-profit entities to use this strain. For more information, please contact Margarita Shabashevich

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
076082-MU-RESUS Litter recovered from cryo-archive $2,624.00 / $5,340.00
Non-Profit / For-Profit
Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.