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Strain Name:
STOCK Hoxa5tm2.1Ljea/Mmucd
Stock Number:
076202-UCD
Citation ID:
RRID:MMRRC_076202-UCD
Other Names:
Hoxa5FLAG

Strain Information

QUALITY CONTROL OF STOCK Hoxa5tm2.1Ljea/Mmucd  |  University of California at Davis
Last Updated:
Strain Data and Information Information by Submitter Assessed by MMRRC1,2
Published Provided
Allele-specific genotype3YES
Genetic backgroundn.d.
Viability of genotypes available for distributionn.d.
Specific Pathogen- Status YES
Recoverability of cryopreserved sperm/embryos4 n.d.
Gene or allele sequence3 n.d.
Gene or allele expression3n.d.
Gene or allele function3n.d.
Observable and/or measurable phenotypesn.d.
Fertility of genotypes available for distributionn.d.
Fecundity/breeding performance n.d.

1 When indicated as "assessed by MMRRC" ("YES"), please note that the information presented on this strain is to the best of our knowledge correct and up-to-date at the the MMRRC Distribution Center assigned to maintain and distribute this strain. This information is subject to change due to breeding, maintenance, and other actions. Please direct any questions on the information presented in this table to the MMRRC Distribution Center.

2 If an assessment has not been performed by the MMRRC (as indicated by "NO"), investigators may request specific testing for a fee. Requests should be submitted directly to the MMRRC Distribution Center assigned to the management, archiving, and distribution of the strain. A full listing of available testing and analytical services is available at https://www.mmrrc.org/about/services.php.

3 This information may or may not apply to each individual engineered allele (e.g., Cre, FlpO) present in the strain.

4 Recovery refers to thawing, in vitro fertilization (IVF), and/or embryo culture leading to live offspring.

Hoxa5tm2.1Ljea
Name:
Type: Allele
Species: Mus musculus (mouse)
Chromosome:
Alteration at locus: Knock-In
Hoxa5
Name: homeobox A5
Synonyms: Hox-1.3
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 15402
HGNC: HGNC:5106
Homologene: 40726
Genetic Alterations
This Hoxa5 epitope-tagged mouse line carries a targeted knock-in mutation of the Hoxa5 gene encoding a HOXA5 protein with a 3xFLAG epitope tag inserted in-frame into the C-terminus.
ES Cell Line
KV1 derived from (C57BL/6J x 129S6/SvEvTac)F1
Phenotype

Homozygous mice are viable, healthy, and fertile showing no overt phenotypes.

HOXA5-FLAG fusion protein is expressed in the same tissues as the endogenous HOXA5 protein.

MeSH Terms
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Gene Expression Regulation, Developmental
  • Animals
  • Mice
  • Mice, Knockout
  • Lung/embryology
  • Lung/growth & development
  • Lung/metabolism
  • Fibroblast Growth Factor 10/genetics
  • Fibroblast Growth Factor 10/metabolism
  • Transcription, Genetic
  • Organogenesis/genetics
  • Chromatin Immunoprecipitation Sequencing
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
  • Fibroblast Growth Factor 10
  • Gene Expression Regulation
  • Homeodomain Proteins
  • Lung embryology
  • Lung growth & development
  • Lung metabolism
  • Organogenesis
  • Transcription Factors
  • Lung Cancer
  • Developmental Biology
  • Internal/Organ
  • Neurobiology
  • Models for Human Disease
  • Research Tools
  • Reproduction
Submitter
Jennifer Mansfield, Ph.D., Barnard College.
Lucie Jeannotte, Ph.D., Laval University.
Primary Reference

Frenette B, Guéno J, Houde N, Mansfield JH, Jeannotte L. HOXA5-driven transcriptional program in lung development. Dev Biol. 2025 Nov;527:191-205. doi: 10.1016/j.ydbio.2025.08.010. Epub 2025 Aug 13. (Medline PMID: 40816456)

Strain Development
A targeting construct was designed in which a 3xFLAG sequence was inserted in-frame into the C-terminus of HOXA5. The vector was targeted into KV1 embryonic stem (ES) cells derived from (C57BL/6J x 129S6/SvEvTac)F1 by standard electroporation and selection techniques. Chimeras generated from a correctly targeted positive ES cell clone were bred to 129S6/SvEvTac mice to establish lines. To remove the neomycin selection cassette and generate mice carrying the Hoxa5FLAG allele, Hoxa5FLAGneo heterozygous mice were crossed with the Sox2cre (B6N.Cg-Edil3Tg(Sox2−cre)1Amc) mouse line. The Sox2cre line was subsequently crossed out after removal of the neomycin selection cassette and is not present in the Hoxa5FLAG strain.

Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Agouti
Eye
Black
MMRRC Breeding System
Sib-mating
Generation
N/A
Overall Breeding Performance
Excellent
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined
Average litter size
7 to 9
Recommended wean age
3 Weeks
Average Pups Weaned
7 to 9

Order Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

A Commercial License Agreement from the Submitter is required for for-profit entities to use this strain. For more information, please contact Office Of The General Counsel.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Submitter is required for for-profit entities to use this strain. For more information, please contact Office Of The General Counsel

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.