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Availability & Fees Order this Strain
1 When indicated as "assessed by MMRRC" ("YES"), please note that the information presented on this strain is to the best of our knowledge correct and up-to-date at the the MMRRC Distribution Center assigned to maintain and distribute this strain. This information is subject to change due to breeding, maintenance, and other actions. Please direct any questions on the information presented in this table to the MMRRC Distribution Center.
2 If an assessment has not been performed by the MMRRC (as indicated by "NO"), investigators may request specific testing for a fee. Requests should be submitted directly to the MMRRC Distribution Center assigned to the management, archiving, and distribution of the strain. A full listing of available testing and analytical services is available at https://www.mmrrc.org/about/services.php.
3 This information may or may not apply to each individual engineered allele (e.g., Cre, FlpO) present in the strain.
4 Recovery refers to thawing, in vitro fertilization (IVF), and/or embryo culture leading to live offspring.
Homozygous conditional mice are viable and fertile and do not exhibit an overt phenotype prior to Cre-mediated recombination.
Conditional Phenotype: Conditional deletion of Kit from the small intestinal epithelium using Vil1-Cre efficiently eliminated epithelial KIT expression. Under homeostatic conditions, these mice showed no significant differences in body weight, small intestinal length, epithelial proliferation, or frequencies of tuft, Paneth, and goblet cells, indicating that epithelial KIT is dispensable for normal small intestinal homeostasis, cellular turnover, and secretory cell differentiation. Following Nippostrongylus brasiliensis (rodent hookworm) infection, epithelial Kit deletion produced an approximately 35% reduction in tuft cell hyperplasia, with the defect most pronounced in the proximal small intestine where the worms reside. Goblet cell hyperplasia, small intestinal length, body weight, and worm burden were not significantly altered with constitutive Vil1-Cre-mediated deletion. Acute epithelial deletion using Vil1-Cre-ERT2 produced a more pronounced, approximately 44% reduction in tuft cell hyperplasia following N. brasiliensis infection, accompanied by a trend toward increased intestinal worm burden.
Tuft cell-specific deletion using Pou2f3-Cre-ERT2 similarly reduced tuft cell hyperplasia and resulted in increased worm burden, demonstrating that KIT is required after commitment to the tuft cell lineage. In contrast, deletion with Dclk1-Cre, which retained KIT expression in many newly generated crypt tuft cells, did not alter tuft cell hyperplasia or worm clearance. These findings indicate that KIT predominantly functions in early committed tuft cells within the crypt and villus base to support their differentiation and/or proliferation.
To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.
The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.
Lara HI, Bell MR, O'Connor S, Ting HA, von Moltke J. KIT supports small intestinal tuft cell hyperplasia. Sci Adv. 2026 Feb 27;12(9):eady0883. doi: 10.1126/sciadv.ady0883. Epub 2026 Feb 25. (Medline PMID: 41739917)
Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
A Commercial License Agreement from the Submitter is required for for-profit entities to use this strain. For more information, please contact James R. Lowry.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
A Commercial License Agreement from the Submitter is required for for-profit entities to use this strain. For more information, please contact James R. Lowry
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
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