Strain Detail Sheet

Strain Name    :

B6.Cg-Mitfmi-sp Mitfmi-vga9/Mmmh

Stock Number :

009963-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Mitfmi-sp
Name: microphthalmia spotted
Chromosome: 6
Alteration at locus: Spontaneous Mutation
Gene Symbol: Mitf
Name: microphthalmia-associated transcription factor
Chromosome: 6
Alteration at locus: Spontaneous Mutation
Allele Symbol: Mitfmi-vga9
Name: transgenic insertion 9
Alteration at locus: Transgenic (random, gene disruption)
Gene Symbol: Mitf
Name: microphthalmia-associated transcription factor
Alteration at locus: Transgenic (random, gene disruption)

Genetic Alterations:
This is a transgenic, knock-in mouse produced by Heinz Arnheitter.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Coat color is used to determine zygosity and the MMRRC PCR (genotyping assay) is used to confirm transgene integration.

Description of White Spotting mutants

Characteristics of the Mitf Locus:
Microphthalmia (Mitf) locus encodes the basic-helix-loop-helix-leucine zipper protein transcription factor MITF (Microphthalmia associated transcription factor). (9, 26, 27). Tissues/organs that may be affected (depending upon the allele) include pigmentation of the skin, pigmentation and development of the eye and ear, mast cells, osteoclasts, and reproductive cells. Mitf is an important transcription factor that functions to initiate transcription at several other loci within the pigment cell, including tyrosinase (tyr) and tyrosinase-related protein I (TyrpI.) and also regulates Bcl2. In other cell types, for example in osteoclasts and mast cells, Mitf functions as transcription factor of several cell-type specific proteins. There is no evidence that the size of white spots on mice mutant at Mitf are influenced by pheomelanin. However, mice that are both recessive yellow and Miwh/+ in genotype are much more eumelanic than mice that are yellow and +/+ (Lamoreux, 1981). Strain background does not appear to influence size or location of spots in mice mutant at Mitf.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Mitfmi-sp/Mitfmi-sp
        B6.Cg-Mitf<Mi-wh>/Mitf<mi-sp>/J
  • pigmentation phenotype
    • pigmentation phenotype (MGI Ref IDs J:12946, J:35685)
      • (MGI Ref ID J:35685)
      • homozygotes have a normal appearance since the mutation is only visibly evident in compound heterozygotes (MGI Ref ID J:12946)
      • the number, kind, and arrangement of cortical pigment granules in the hair is normal (MGI Ref ID J:12946)
      • hypopigmentation (MGI Ref ID J:12946)
        • despite the normal black coat color, homozygotes have reduced tyrosinase activity as evidenced by reduced incorporation of radiolabeled tyrosine into skin slices from 5 day old homozygotes
Mitfmi-vga9/Mitfmi-vga9
        involves: C57BL/6 * CBA
  • pigmentation phenotype
    • absent coat pigmentation (MGI Ref ID J:89821)
      • white coat
  • vision/eye phenotype
    • microphthalmia (MGI Ref ID J:89821)
  • integument phenotype
    • absent coat pigmentation (MGI Ref ID J:89821)
      • white coat

Strain of Origin: C57BL/6J-MitfMi-wh, C57BL/6 x CBA

Strain genetic background: C57BL/6

Strain Development: The mice were backcross to C57BL/6 approximately seven generations, followed by intercrossing for approximately four generations. In January 2003 they were backcrossed using B6-mi*sp and this backcrossing continued to the mice that were shipped to MMRRC in 2004.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Pigmentation
  • Neural Crest Development
  • Cell Survival
  • Development of Eye
  • Deafness
  • Peristalsis
  • Mast Cells
  • Osteoclasts
  • Reproductive Cells
  • Transcription Factors
  • Important Regulatory Molecular

Strain Origin

Donor: Dr. M. L. Lamoreux, Texas A&M University

Primary Reference:

  • Hemesath TJ, Steingrimsson E, McGill G, Hansen MJ, Vaught J, Hodgkinson CA, Arnheiter H, Copeland NG, Jenkins NA, Fisher DE. microphthalmia, a critical factor in melanocyte development, defines a discrete transcription factor family. Genes Dev. 1994 Nov 15;8(22):2770-80. (Medline PMID: 7958932 )
  • Steingrimsson E, Moore KJ, Lamoreux ML, Ferre-D'Amare AR, Burley SK, Zimring DC, Skow LC, Hodgkinson CA, Arnheiter H, Copeland NG. Molecular basis of mouse microphthalmia (mi) mutations helps explain their developmental and phenotypic consequences. Nat Genet. 1994 Nov;8(3):256-63. (Medline PMID: 7874168)
  • Tachibana M, Perez-Jurado LA, Nakayama A, Hodgkinson CA, Li X, Schneider M, Miki T, Fex J, Francke U, Arnheiter H. Cloning of MITF, the human homolog of the mouse microphthalmia gene and assignment to chromosome 3p14.1-p12.3. Hum Mol Genet. 1994 Apr;3(4):553-7. (Medline PMID: 8069297)

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat color: See photo and strain description

Other:


Breeding

MMRRC Breeding System: Sib mating

Breeding Scheme(s):

  • Wildtype littermate female x Heterozygous male
  • Heterozygous female x Wildtype littermate male

Generation: N>10

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 24 weeks 24 weeks

Average litter size: 6

Recommended wean age: 3 - 4 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
009963-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
009963-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2
009963-MU-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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