Strain Detail Sheet

Strain Name    :

STOCK Krt25Re In(11Trp53;11Wnt3)8Brd/Mmucd

Stock Number :

009973-UCD

Other Names   :

Chr11-1/Re, STOCK Krt25Re In(11Trp53;11Wnt3)8Brd/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Telomeric end point: Wnt3In(11Trp53;11Wnt3)8Brd
Name: inversion, Chr 11, Allan Bradley 8
Chromosome: 11
Alteration at locus: Inversion
Centomeric end point: Trp53In(11Trp53;11Wnt3)8Brd
Name: inversion, Chr 11, Allan Bradley 8
Chromosome: 11
Alteration at locus: Inversion
Allele Symbol: Krt25Re
Name: rex
Alteration at locus: Spontaneous Mutation
Gene Symbol: Krt25
Name: keratin 25
Chromosome: 11
Alteration at locus: Spontaneous Mutation

Genetic Alterations:
Spontaneous mutation/Inversion mutation

Genotype Determination:

  • Genotype by Phenotype. See below.

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Heterozygotes: This 24 cM inversion is used as a "balancer" for chromosome 11 mutations in mutagenesis screens. The inversion disrupts Wnt3 and is lethal in the homozygous state. The normal copy of Chr 11 carries the the curly coat mutation, rex. Mice heterozygous for both mutations are yellow with a curly coat. When combined with ENU mutagenized C57BL/6 males, progeny can be identified by phenotype (see mating scheme).

Homozygotes: lethal


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
In(11Trp53;11Wnt3)8Brd/In(11Trp53;11Wnt3)8Brd
        involves: 129S7/SvEvBrd
  • mortality/aging
    • complete prenatal lethality (MGI Ref ID J:56548)
      • homozygous embryos are inviable
Krt25Re/Krt25+
        Background Not Specified
  • integument phenotype
    • abnormal hair shaft morphology (MGI Ref ID J:15247)
      • the hair shaft is irregular
    • abnormal hair texture (MGI Ref ID J:15328)
      • rough coat (MGI Ref ID J:15328)
        • mice have roughish coats
    • curly vibrissae (MGI Ref ID J:15328)
      • vibrassae are curly
    • increased curvature of guard hairs (MGI Ref ID J:15328)
      • mice exhibit short, curly and irregular guard hairs
    • waved hair (MGI Ref ID J:15328)
      • mice exhibit wavy coats when young but not during adulthood
Krt25Re/Krt25Re
        Background Not Specified
  • integument phenotype
    • abnormal hair follicle inner root sheath morphology (MGI Ref ID J:15247)
      • the layers of the inner root sheath are irregular
    • abnormal hair shaft morphology (MGI Ref ID J:15247)
      • the hair shaft is irregular
    • abnormal hair texture (MGI Ref ID J:15328)
      • rough coat (MGI Ref ID J:15328)
        • mice have roughish coats
    • curly vibrissae (MGI Ref ID J:15328)
      • vibrassae are curly
    • increased curvature of guard hairs (MGI Ref ID J:15328)
      • mice exhibit short, curly and irregular guard hairs
    • waved hair (MGI Ref ID J:15328)
      • mice exhibit wavy coats when young but not during adulthood

Suggested Control Mice:

  • Wildtype littermates

Research Applications

    Strain Origin

    Donor: Monica J. Justice, Ph.D., Baylor College of Medicine

    Primary Reference:

    Kile BT, Hentges KE, Clark AT, Nakamura H, Salinger AP, Liu B, Box N, Stockton DW, Johnson RL, Behringer RR, Bradley A, Justice MJ. Functional genetic analysis of mouse chromosome 11. Nature. 2003 Sep 4;425(6953):81-6. (Medline PMID: 12955145)

    Colony and Husbandry Information

    Special Considerations

    These mice are reporter mice to be used with strains containing mutations of chromosome 11 as noted in the reference text.

    Health Status Report

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

    Appearance

    Coat color: Agouti plus curly coat and yellow hair on ears and tail

    Other:


    Breeding

    MMRRC Breeding System: Sib mating

    Breeding Scheme(s):

    • Hetero/Hetero female (curly+yellow) x Hetero/Hetero male (curly+ yellow)
    • View Mating Scheme

    Generation: Unknown

    Overall Breeding Performance: Fair to Poor

    Reproductive Statistics

    Viability and Fertility:FemaleMale
    Homozygotes are viable: No No
    Homozygotes are fertile: No No
    Heterozygotes are fertile: Yes Yes
     
    Age Reproductive Decline: 40 weeks 40 weeks

    Average litter size: 4

    Recommended wean age: 3 -4 weeks

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Conditions of Distribution [Including applicable technology transfer agreements]

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # - Description Distribution
    Fee/unit (US $)
    Units Notes
    009973-UCD-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.
    009973-UCD-SPERMCryo-preserved spermatozoa
    $437.00
    Non-Profit
    Aliquot Approximate quantity.2
    009973-UCD-EMBRYOCryo-preserved embryos
    $1,038.00
    Non-Profit
    Aliquot Approximate quantity3: 20-40 embryos / aliquot

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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