Strain Detail Sheet

Strain Name    :

B6.129-Cacna1htm1Kcam/Mmmh

Stock Number :

009979-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Cacna1htm1Kcam
Name: targeted mutation 1, Kevin P Campbell
Alteration at locus: Knockout
Gene Symbol: Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Chromosome: 17
Alteration at locus: Knockout

Genetic Alterations:
The long arm of homology in the targeting vector was a 4.5 kb NotI/EcoRI fragment upstream of the IS4 region of alpha1 3.2 subunit, which has been subcloned into pBluescript KS (+) (pBS) and cut with NotI/ClaI. The short arm was a 2.2 kb BamHI/BamHI fragment downstream of the extracellular loop between IS5 and IS6. These fragments were inserted into cloning sites of pPNT flanking a reversed PGK-neomycin resistance cassette. The vector included a thymidine kinase cassette distal to the short arm to allow for negative selection of non-recombinants.

Genotype Determination:

ES Cell Line: R1 derived from C57BL/6 x 129

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: alpha1 3.2-null mice have constitutively constricted coronary arterioles and focal myocardial fibrosis. Coronary arteries isolated from alpha1 3.2-null arteries showed normal contractile responses but reduced relaxation in response to acetylcholine and nitroprusside.

Heterozygous phenotype: Heterozygotes do not show any morphological abnormalities.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Cacna1htm1Kcam/Cacna1htm1Kcam
        involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
  • cardiovascular system phenotype
    • abnormal cardiovascular system morphology (MGI Ref ID J:86616)
      • abnormal blood vessel morphology (MGI Ref ID J:86616)
        • vessels in the heart have irregular spiral shapes
        • abnormal arteriole morphology (MGI Ref ID J:86616)
          • exhibit constitutively constricted coronary arterioles
      • abnormal myocardial fiber morphology (MGI Ref ID J:86616)
        • focal myocardial fibrosis
      • cardiac fibrosis (MGI Ref ID J:86616)
        • exhibit progressive cardiac fibrosis starting at 10 weeks of age; hearts show large areas of fibrosis, necrosis, and lymphoctye infiltration
    • increased vasoconstriction (MGI Ref ID J:86616)
      • vessels in the heart and coronary arterioles are constitutively contracted
      • isolated coronary arteries show normal contractile responses but show reduced relaxation in response to acetylcholine and ntiroprusside
  • growth/size phenotype
    • decreased body size (MGI Ref ID J:86616)
      • both males and females are smaller
  • nervous system pheno type
    • abnormal channel response (MGI Ref ID J:86616)
      • the low voltage-activated calcium current (IT is significantly diminished in dorsal root ganglia neurons, however no difference in the high voltage-activated calcium currents
  • muscle phenotype
    • abnormal myocardial fiber morphology (MGI Ref ID J:86616)
      • focal myocardial fibrosis
    • increased vasoconstriction (MGI Ref ID J:86616)
      • vessels in the heart and coronary arterioles are constitutively contracted
      • isolated coronary arteries show normal contractile responses but show reduced relaxation in response to acetylcholine and ntiroprusside

Strain of Origin: C57BL/6x129

Strain genetic background: C57BL/6

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Cardiovascular
  • Cell Biology
  • Models for Human Disease
  • Neurobiology
  • Reproduction

Strain Origin

Donor: Kevin P. Campbell, Ph.D., University of Iowa

Primary Reference:

Chen CC, Lamping KG, Nuno DW, Barresi R, Prouty SJ, Lavoie JL, Cribbs LL, England SK, Sigmund CD, Weiss RM, Williamson RA, Hill JA, Campbell KP. Abnormal coronary function in mice deficient in alpha1H T-type Ca2+ channels. Science. 2003 Nov 21;302(5649):1416-8. (Medline PMID: 14631046)

Colony and Husbandry Information

Special Considerations

The use of mouse houses help to facilitate better reproductive results. Once the pups are weaned at 21 days, they should be grueled for about one week.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
009979-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
009979-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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