Allele Symbol: Cacna1htm1Kcam
Name: targeted mutation 1, Kevin P Campbell
Alteration at locus: Knockout
Gene Symbol: Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Alteration at locus: Knockout
ES Cell Line: R1 derived from C57BL/6 x 129
Homozygous phenotype: alpha1 3.2-null mice have constitutively constricted coronary arterioles and focal
myocardial fibrosis. Coronary arteries isolated from alpha1 3.2-null arteries showed
normal contractile responses but reduced relaxation in response to acetylcholine
Heterozygous phenotype: Heterozygotes do not show any morphological abnormalities.
Strain of Origin: C57BL/6x129
Strain genetic background: C57BL/6
Suggested Control Mice:
Donor: Kevin P. Campbell, Ph.D., University of Iowa
Chen CC, Lamping KG, Nuno DW, Barresi R, Prouty SJ, Lavoie JL, Cribbs LL, England SK, Sigmund CD, Weiss RM, Williamson RA, Hill JA, Campbell KP. Abnormal coronary function in mice deficient in alpha1H T-type Ca2+ channels. Science. 2003 Nov 21;302(5649):1416-8. (Medline PMID: 14631046)
The use of mouse houses help to facilitate better reproductive results. Once the pups are weaned at 21 days, they should be grueled for about one week.
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.