Strain Name:
B6.129-Cacna1htm1Kcam/Mmmh
Stock Number:
009979-MU
Citation ID:
RRID:MMRRC_009979-MU

Gene Information

Cacna1htm1Kcam
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit; targeted mutation 1, Kevin P Campbell
Synonyms: alpha13.2-, Cav3.2-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Knockout
Cacna1h
Name: calcium channel, voltage-dependent, T type, alpha 1H subunit
Synonyms: Cav3.2, alpha13.2, T-type Cav3.2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Knockout
NCBI: 58226
HGNC: HGNC:1395
Homologene: 56913
Genetic Alterations
The long arm of homology in the targeting vector was a 4.5 kb NotI/EcoRI fragment upstream of the IS4 region of alpha1 3.2 subunit, which has been subcloned into pBluescript KS (+) (pBS) and cut with NotI/ClaI. The short arm was a 2.2 kb BamHI/BamHI fragment downstream of the extracellular loop between IS5 and IS6. These fragments were inserted into cloning sites of pPNT flanking a reversed PGK-neomycin resistance cassette. The vector included a thymidine kinase cassette distal to the short arm to allow for negative selection of non-recombinants.
Genotype Determination
ES Cell Line
R1 derived from (129X1/SvJ x 129S1/Sv)F1-Kitl+
Phenotype
Homozygous: alpha1 3.2-null mice have constitutively constricted coronary arterioles and focal myocardial fibrosis. Coronary arteries isolated from alpha1 3.2-null arteries showed normal contractile responses but reduced relaxation in response to acetylcholine and nitroprusside.

Heterozygous: Heterozygotes do not show any morphological abnormalities.
Mammalian Phenotype Terms
Allelic Composition: Cacna1htm1Kcam/Cacna1htm1Kcam (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6J )

Allelic Composition: Cacna1htm1Kcam/Cacna1htm1Kcam (Genetic Background: involves: 129S1/Sv * 129X1/SvJ * ICR )

MeSH Terms
  • Acetylcholine/pharmacology
  • Animals
  • Arteries/drug effects
  • Arteries/physiology
  • Calcium/metabolism
  • Calcium Channels, T-Type/genetics
  • Calcium Channels, T-Type/physiology
  • Coronary Vessels/drug effects
  • Coronary Vessels/pathology
  • Coronary Vessels/physiology
  • Echocardiography
  • Electrocardiography
  • Endothelium, Vascular/drug effects
  • Endothelium, Vascular/physiology
  • Female
  • Fibrosis
  • Ganglia, Spinal/cytology
  • Gene Targeting
  • Heart/physiology
  • Heart Rate
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Muscle, Smooth, Vascular/physiology
  • Myocardium/pathology
  • Neurons/metabolism
  • Nickel/pharmacology
  • Nitric Oxide/physiology
  • Nitric Oxide Donors/pharmacology
  • Nitroprusside/pharmacology
  • Patch-Clamp Techniques
  • Vasoconstriction/drug effects
  • Vasodilation/drug effects
Suggested Control Mice
  • Wildtype littermates
  • Cardiovascular
  • Cell Biology
  • Models for Human Disease
  • Neurobiology
  • Reproduction
Donor
Kevin P. Campbell, Ph.D., University of Iowa
Primary Reference
Chen CC, Lamping KG, Nuno DW, Barresi R, Prouty SJ, Lavoie JL, Cribbs LL, England SK, Sigmund CD, Weiss RM, Williamson RA, Hill JA, Campbell KP. Abnormal coronary function in mice deficient in alpha1H T-type Ca2+ channels. Science. 2003 Nov 21;302(5649):1416-8. (Medline PMID: 14631046)

Colony and Husbandry Information

The use of mouse houses help to facilitate better reproductive results. Once the pups are weaned at 21 days, they should be grueled for about one week.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
009979-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
009979-MU-RESUS Litter recovered from cryo-archive $2,016.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.