Strain Name:
B6.129X1-Gpd2tm1Lbr/Mmucd
Stock Number:
009980-UCD
Citation ID:
RRID:MMRRC_009980-UCD
Other Names:
mGPD KO, B6.129X1-Gpd2tm1Lbr/Mmcd

Strain Information

Gpd2tm1Lbr
Name: glycerol phosphate dehydrogenase 2, mitochondrial; targeted mutation 1, Laura J Brown
Synonyms: Gdm-, mGPD o/o
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 2
Alteration at locus: Knockout
Gpd2
Name: glycerol phosphate dehydrogenase 2, mitochondrial
Synonyms: Gdm1
Type: Gene
Species: Mouse
Chromosome: 2
Alteration at locus: Knockout
NCBI: 14571
HGNC: HGNC:4456
Homologene: 352
Genetic Alterations
Exons 5 and 6 of the (nuclearly-encoded) mitochondrial glycerol phosphate dehydrogenase gene were replaced by a neo cassette.
Genotype Determination
ES Cell Line
RW-4 derived from 129X1/SvJ
Phenotype
Homozygotes: In crosses of heterozygous mice, both male and female homozygous knockout mice have lower body weight than wild type littermates . On the B6 background there is reduced viability of homozygous mice, both in numbers born and numbers weaned. Nursing Gdm1 -/- pups have lower liver ATP and slightly higher glycerol phosphate than controls. Adult homozygous animals have a 40% reduction in the weight of white adipose tissue, and lower fasting blood glucose, compared with controls. The combination of homozygous Gdm1 knockout with homozygous loss of the cytosolic glycerol phosphate dehydrogenase (Gdc1, found in BALB/cHeA) is lethal in the newborn period. Pups with the combined defects have severe hypoglycemia, ketosis, and glyceroluria.

Heterozygotes:
In F2 and F3 animals the body weights appeared intermediate. Not extensively studied.
Mammalian Phenotype Terms
Allelic Composition: Gpd2tm1Lbr/Gpd2tm1Lbr (Genetic Background: B6.129X1-Gpd2tm1Lbr )

Allelic Composition: Gpd2tm1Lbr/Gpd2tm1Lbr (Genetic Background: involves: 129X1/SvJ * C57BL/6J )

Allelic Composition: Gpd2tm1Lbr/Gpd2+ (Genetic Background: involves: 129X1/SvJ * C57BL/6J )

MeSH Terms
  • Adipose Tissue/cytology
  • Adipose Tissue/growth & development
  • Animals
  • Blotting, Western
  • Body Weight
  • Cytosol/enzymology
  • Female
  • Genetic Vectors
  • Genotype
  • Glycerol/metabolism
  • Glycerolphosphate Dehydrogenase/genetics
  • Glycerolphosphate Dehydrogenase/physiology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mitochondria/enzymology
  • Models, Biological
  • Models, Genetic
  • RNA, Messenger/metabolism
  • Survival Analysis
  • Temperature
  • Thermogenesis
  • Thyroid Gland/physiology
  • Time Factors
  • Glycerol/blood
  • Glycerol/urine
  • Hypoglycemia/etiology
  • Hypoglycemia/metabolism
  • Hypoglycemia/mortality
  • Ketosis/etiology
  • Ketosis/metabolism
  • Ketosis/mortality
  • Liver/metabolism
  • Mice, Inbred BALB C
  • Models, Chemical
  • Body Weight/physiology
  • Caloric Restriction
  • Calorimetry, Indirect
  • Diet
  • Dietary Carbohydrates/pharmacology
  • Dietary Fats/pharmacology
  • Eating/physiology
  • Energy Metabolism/genetics
  • Energy Metabolism/physiology
  • Gene Deletion
  • Glycerolphosphate Dehydrogenase/deficiency
  • Phenotype
  • RNA, Messenger/biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sex Characteristics
  • Thermogenesis/genetics
  • Thermogenesis/physiology
  • Triglycerides/biosynthesis
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@ucdavis.edu. Older strains may not have this information.
  • Diabetes/Obesity
  • Metabolism
  • Reproduction
Donor
Laura J. Brown, MD, PhD, University of Wisconsin
Primary Reference
  • Brown LJ, Koza RA, Everett C, Reitman ML, Marshall L, Fahien LA, Kozak LP, MacDonald MJ. Normal thyroid thermogenesis but reduced viability and adiposity in mice lacking the mitochondrial glycerol phosphate dehydrogenase. J Biol Chem. 2002 Sep 6;277(36):32892-8. Epub 2002 Jul 01. (Medline PMID: 12093799)
  • Brown LJ, Koza RA, Marshall L, Kozak LP, MacDonald MJ. Lethal hypoglycemic ketosis and glyceroluria in mice lacking both the mitochondrial and the cytosolic glycerol phosphate dehydrogenases. J Biol Chem. 2002 Sep 6;277(36):32899-904. Epub 2002 Jul 01. (Medline PMID: 12093800)
  • Alfadda A, DosSantos RA, Stepanyan Z, Marrif H, Silva JE. Mice with deletion of the mitochondrial glycerol-3-phosphate dehydrogenase gene exhibit a thrifty phenotype: effect of gender. Am J Physiol Regul Integr Comp Physiol. 2004 Jul;287(1):R147-56. Epub 2004 Mar 18. (Medline PMID: 15031134)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.
Coat Color
Black
MMRRC Breeding System
The MMRRC has always maintained this strain in a cryopreserved format. Information in the "Breeding" and "Reproductive statistics" sections has been provided by the Donating Investigator
Breeding Scheme(s)
  • Homozygous female x Homozygous male
Generation
N5 As of: 2003
Overall Breeding Performance
Poor
Average litter size
5
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@ucdavis.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
009980-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / Non-Profit Aliquot Approximate quantity2 : 20-40 embryos / aliquot
009980-UCD-SPERM Cryo-preserved spermatozoa $546.25 / Non-Profit Aliquot Approximate quantity3
009980-UCD-RESUS Litter recovered from cryo-archive $4,044.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.