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Strain Name:
C57BL/6J-MtgxR0013Btlr/Mmmh
Stock Number:
038308-MU
Citation ID:
RRID:MMRRC_038308-MU
Other Names:
R0013 (G1), C57BL/6J-MtgxR0013Btlr
Major Collection:

Strain Information

Prkaca
Name: protein kinase, cAMP dependent, catalytic, alpha
Synonyms: Cs, C alpha, PKA, Pkaca
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 18747
HGNC: HGNC:9380
Homologene: 121574
Faah
Name: fatty acid amide hydrolase
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 14073
HGNC: HGNC:3553
Homologene: 68184
Slit3
Name: slit guidance ligand 3
Synonyms: Slit1, b2b2362.1Clo
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 20564
Homologene: 2303
Dnmbp
Name: dynamin binding protein
Synonyms: 2410003M15Rik, Tuba, 2410003L07Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 71972
Homologene: 9061
Ppm1e
Name: protein phosphatase 1E (PP2C domain containing)
Synonyms: PP2CH, POPX1, B930008A12Rik
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 320472
Homologene: 22848
Rngtt
Name: RNA guanylyltransferase and 5'-phosphatase
Synonyms: mouse capping enzyme
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 24018
Homologene: 37851
Stk17b
Name: serine/threonine kinase 17b (apoptosis-inducing)
Synonyms: Drak2, 3110009A03Rik
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 98267
Homologene: 31231
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 8,463,462 bp
  • T to C, chromosome 1 at 53,764,132 bp
  • T to A, chromosome 1 at 71,654,308 bp
  • C to T, chromosome 1 at 84,494,893 bp
  • C to T, chromosome 1 at 86,529,776 bp
  • T to A, chromosome 1 at 134,290,235 bp
  • T to C, chromosome 1 at 138,113,559 bp
  • A to G, chromosome 2 at 26,473,818 bp
  • A to G, chromosome 2 at 36,870,160 bp
  • C to A, chromosome 2 at 76,739,158 bp
  • C to T, chromosome 2 at 76,907,752 bp
  • T to C, chromosome 2 at 88,034,266 bp
  • T to A, chromosome 2 at 121,076,882 bp
  • G to T, chromosome 2 at 164,168,643 bp
  • C to A, chromosome 2 at 181,232,759 bp
  • A to T, chromosome 2 at 181,240,959 bp
  • A to G, chromosome 3 at 72,910,476 bp
  • T to C, chromosome 3 at 88,349,233 bp
  • A to T, chromosome 3 at 116,776,608 bp
  • T to A, chromosome 4 at 33,379,409 bp
  • T to C, chromosome 4 at 41,765,060 bp
  • T to C, chromosome 4 at 59,213,931 bp
  • T to A, chromosome 4 at 108,530,955 bp
  • C to A, chromosome 4 at 116,004,391 bp
  • C to A, chromosome 4 at 133,584,792 bp
  • T to C, chromosome 4 at 138,175,848 bp
  • T to C, chromosome 4 at 138,317,401 bp
  • T to C, chromosome 4 at 155,887,168 bp
  • T to A, chromosome 5 at 32,349,615 bp
  • T to C, chromosome 5 at 34,820,104 bp
  • G to A, chromosome 5 at 62,683,484 bp
  • A to G, chromosome 5 at 97,979,985 bp
  • G to T, chromosome 5 at 103,828,484 bp
  • A to G, chromosome 5 at 114,857,347 bp
  • A to G, chromosome 5 at 147,571,014 bp
  • T to C, chromosome 6 at 123,202,149 bp
  • A to G, chromosome 7 at 3,683,822 bp
  • T to A, chromosome 7 at 26,571,225 bp
  • A to G, chromosome 7 at 27,636,058 bp
  • T to C, chromosome 7 at 41,044,292 bp
  • T to C, chromosome 7 at 72,229,408 bp
  • T to A, chromosome 7 at 100,416,062 bp
  • T to A, chromosome 7 at 105,755,836 bp
  • T to C, chromosome 7 at 111,554,457 bp
  • A to G, chromosome 8 at 71,684,327 bp
  • G to A, chromosome 8 at 83,988,303 bp
  • G to T, chromosome 8 at 105,375,396 bp
  • T to C, chromosome 9 at 37,542,576 bp
  • A to T, chromosome 9 at 40,006,355 bp
  • G to A, chromosome 9 at 53,912,901 bp
  • A to T, chromosome 9 at 59,860,206 bp
  • A to T, chromosome 9 at 62,776,613 bp
  • A to T, chromosome 9 at 107,978,249 bp
  • G to T, chromosome 9 at 110,850,055 bp
  • A to T, chromosome 9 at 123,822,406 bp
  • T to C, chromosome 10 at 60,413,173 bp
  • A to G, chromosome 10 at 76,457,512 bp
  • A to G, chromosome 11 at 35,707,918 bp
  • A to G, chromosome 11 at 46,406,804 bp
  • C to A, chromosome 11 at 67,709,712 bp
  • A to G, chromosome 11 at 70,349,635 bp
  • A to T, chromosome 11 at 70,993,635 bp
  • T to A, chromosome 11 at 74,289,051 bp
  • C to T, chromosome 11 at 79,689,653 bp
  • A to G, chromosome 11 at 87,249,058 bp
  • C to A, chromosome 11 at 102,005,425 bp
  • A to G, chromosome 11 at 106,348,405 bp
  • T to A, chromosome 12 at 110,868,386 bp
  • G to T, chromosome 12 at 112,177,880 bp
  • A to G, chromosome 13 at 74,021,360 bp
  • T to A, chromosome 15 at 74,608,237 bp
  • T to C, chromosome 15 at 76,178,246 bp
  • T to A, chromosome 16 at 13,813,113 bp
  • T to C, chromosome 16 at 37,061,839 bp
  • C to T, chromosome 16 at 91,651,662 bp
  • A to G, chromosome 17 at 27,431,575 bp
  • A to G, chromosome 17 at 28,217,092 bp
  • G to A, chromosome 17 at 34,515,531 bp
  • G to A, chromosome 18 at 73,590,551 bp
  • A to T, chromosome 18 at 80,129,745 bp
  • A to C, chromosome 19 at 24,733,540 bp
  • A to G, chromosome 19 at 36,958,373 bp
  • G to A, chromosome 19 at 43,902,231 bp
  • A to T, chromosome Y at 941,715 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0013 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038308-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.