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Strain Name:
C57BL/6J-MtgxR0058Btlr/Mmmh
Stock Number:
038352-MU
Citation ID:
RRID:MMRRC_038352-MU
Other Names:
R0058 (G1), C57BL/6J-MtgxR0058Btlr
Major Collection:

Strain Information

Hapln1
Name: hyaluronan and proteoglycan link protein 1
Synonyms: link protein, cartilage linking protein 1, Crtl1l, Crtl1, CLP, LP-1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 12950
VEGA: 13
HGNC: HGNC:2380
Homologene: 1420
Pkd1
Name: polycystin 1, transient receptor potential channel interacting
Synonyms: polycystin-1, PC1, PC-1
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18763
VEGA: 17
HGNC: HGNC:9008
Homologene: 250
Irx3
Name: Iroquois related homeobox 3
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 16373
Homologene: 7385
Tlr9
Name: toll-like receptor 9
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 81897
Homologene: 68126
Avpr1b
Name: arginine vasopressin receptor 1B
Synonyms: V3/V1b pituitary vasopressin receptor, V3/V1b, V1bR, AVPR3, V1BR, VPR3
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 26361
HGNC: HGNC:896
Homologene: 22678
Dst
Name: dystonin
Synonyms: bullous pemphigoid antigen 1, BPAG1-n, BPAG1, Bpag1, Bpag, ah, bullous pemphigoid antigen 1, athetoid, Macf2, 2310001O04Rik, nmf203, A830042E19Rik, nmf339
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13518
HGNC: HGNC:1090
Homologene: 136716
Cadm1
Name: cell adhesion molecule 1
Synonyms: 3100001I08Rik, 2900073G06Rik, RA175N, RA175C, RA175B, RA175A, Necl2, SgIGSF, Tslc1, SynCam, Igsf4, Igsf4a
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 54725
HGNC: HGNC:5951
Homologene: 8641
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 34,006,224 bp
  • A to G, chromosome 1 at 131,599,786 bp
  • C to T, chromosome 1 at 162,698,884 bp
  • A to T, chromosome 1 at 162,886,324 bp
  • T to C, chromosome 2 at 14,138,141 bp
  • T to C, chromosome 2 at 29,993,696 bp
  • A to G, chromosome 2 at 142,857,305 bp
  • A to T, chromosome 2 at 150,628,539 bp
  • G to A, chromosome 2 at 154,206,540 bp
  • T to C, chromosome 3 at 40,805,872 bp
  • A to G, chromosome 4 at 109,736,624 bp
  • A to T, chromosome 4 at 113,046,815 bp
  • G to T, chromosome 4 at 115,041,298 bp
  • A to G, chromosome 4 at 118,809,480 bp
  • G to A, chromosome 4 at 121,920,736 bp
  • A to T, chromosome 5 at 106,637,003 bp
  • T to A, chromosome 5 at 110,202,777 bp
  • T to A, chromosome 5 at 113,285,087 bp
  • G to A, chromosome 5 at 124,124,030 bp
  • A to T, chromosome 5 at 134,659,871 bp
  • T to G, chromosome 5 at 137,290,842 bp
  • A to G, chromosome 5 at 137,310,845 bp
  • C to A, chromosome 5 at 146,956,921 bp
  • G to A, chromosome 6 at 70,356,121 bp
  • A to G, chromosome 6 at 97,423,499 bp
  • C to T, chromosome 7 at 14,664,388 bp
  • C to T, chromosome 7 at 23,929,167 bp
  • T to C, chromosome 7 at 25,715,980 bp
  • T to C, chromosome 7 at 56,170,483 bp
  • G to A, chromosome 7 at 84,347,530 bp
  • G to A, chromosome 7 at 135,108,761 bp
  • T to C, chromosome 8 at 3,688,111 bp
  • A to G, chromosome 8 at 23,229,510 bp
  • C to T, chromosome 8 at 33,581,502 bp
  • T to C, chromosome 8 at 43,185,269 bp
  • T to A, chromosome 8 at 45,785,254 bp
  • C to A, chromosome 8 at 45,796,263 bp
  • C to A, chromosome 8 at 77,667,322 bp
  • T to C, chromosome 8 at 91,800,540 bp
  • G to A, chromosome 8 at 112,785,784 bp
  • A to T, chromosome 9 at 47,850,331 bp
  • C to A, chromosome 9 at 71,641,397 bp
  • T to A, chromosome 9 at 71,724,840 bp
  • T to G, chromosome 9 at 106,224,965 bp
  • A to G, chromosome 9 at 109,328,211 bp
  • T to A, chromosome 9 at 110,594,426 bp
  • T to A, chromosome 10 at 30,647,541 bp
  • T to A, chromosome 10 at 58,480,531 bp
  • T to C, chromosome 10 at 77,869,631 bp
  • T to C, chromosome 10 at 80,261,581 bp
  • A to G, chromosome 11 at 5,630,691 bp
  • C to T, chromosome 11 at 55,221,994 bp
  • A to T, chromosome 11 at 58,625,668 bp
  • A to T, chromosome 11 at 107,672,558 bp
  • A to T, chromosome 13 at 17,672,581 bp
  • A to G, chromosome 13 at 81,182,672 bp
  • T to C, chromosome 13 at 89,607,878 bp
  • A to G, chromosome 14 at 37,131,592 bp
  • A to G, chromosome 14 at 50,834,065 bp
  • C to T, chromosome 14 at 54,963,404 bp
  • A to T, chromosome 14 at 67,781,036 bp
  • A to G, chromosome 15 at 100,215,240 bp
  • C to T, chromosome 16 at 14,142,534 bp
  • A to T, chromosome 16 at 16,863,876 bp
  • A to G, chromosome 16 at 26,524,829 bp
  • A to G, chromosome 16 at 36,754,547 bp
  • T to A, chromosome 16 at 37,142,374 bp
  • G to C, chromosome 17 at 24,564,703 bp
  • G to A, chromosome 18 at 9,213,985 bp
  • A to G, chromosome 18 at 11,923,413 bp
  • G to A, chromosome 18 at 75,043,172 bp
  • A to T, chromosome 19 at 20,383,777 bp
  • A to G, chromosome 19 at 38,525,184 bp
  • T to C, chromosome 19 at 60,874,512 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0058 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038352-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.