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Strain Name:
C57BL/6J-MtgxR0726Btlr/Mmmh
Stock Number:
038908-MU
Citation ID:
RRID:MMRRC_038908-MU
Other Names:
R0726 (G1), C57BL/6J-MtgxR0726Btlr
Major Collection:

Strain Information

Smarca4
Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms: SNF2beta, Brg1, SW1/SNF, b2b692Clo, b2b508.1Clo
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 20586
Homologene: 135927
Gabra6
Name: gamma-aminobutyric acid type A receptor subunit alpha 6
Synonyms: Gabra-6, alpha6, GABA-ARalpha6
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 14399
HGNC: HGNC:4080
Homologene: 20220
Espl1
Name: extra spindle pole bodies 1, separase
Synonyms: separase, SSE, ESP1, PRCE, PRCE, Cerp
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 105988
VEGA: 15
Homologene: 32151
Vldlr
Name: very low density lipoprotein receptor
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 22359
Homologene: 443
Frem2
Name: Fras1 related extracellular matrix protein 2
Synonyms: 8430406N05Rik, 6030440P17Rik, my, ne, b2b1562Clo
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 242022
Homologene: 18454
Pip5k1b
Name: phosphatidylinositol-4-phosphate 5-kinase, type 1 beta
Synonyms: Pipk5b
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 18719
HGNC: HGNC:8995
Homologene: 100644
Usp28
Name: ubiquitin specific peptidase 28
Synonyms: 9830148O20Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 235323
VEGA: 9
Homologene: 10840
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 58,334,782 bp
  • C to T, chromosome 1 at 153,802,328 bp
  • G to A, chromosome 1 at 172,325,921 bp
  • C to T, chromosome 2 at 52,110,353 bp
  • A to T, chromosome 2 at 88,746,008 bp
  • T to A, chromosome 2 at 90,149,283 bp
  • G to A, chromosome 2 at 130,390,939 bp
  • G to T, chromosome 2 at 157,060,262 bp
  • T to C, chromosome 3 at 53,519,626 bp
  • C to T, chromosome 3 at 64,503,765 bp
  • G to A, chromosome 3 at 90,021,246 bp
  • A to T, chromosome 3 at 101,020,622 bp
  • G to A, chromosome 3 at 133,468,184 bp
  • C to T, chromosome 4 at 20,158,388 bp
  • A to G, chromosome 4 at 21,683,914 bp
  • C to T, chromosome 4 at 59,207,798 bp
  • C to A, chromosome 4 at 101,764,934 bp
  • A to T, chromosome 4 at 114,925,250 bp
  • T to C, chromosome 5 at 33,861,028 bp
  • T to A, chromosome 5 at 97,087,494 bp
  • A to T, chromosome 5 at 118,748,684 bp
  • T to C, chromosome 5 at 143,692,642 bp
  • C to T, chromosome 6 at 8,014,495 bp
  • G to T, chromosome 6 at 39,383,959 bp
  • C to T, chromosome 6 at 39,662,148 bp
  • T to A, chromosome 6 at 132,762,452 bp
  • A to G, chromosome 7 at 16,120,722 bp
  • G to T, chromosome 7 at 24,638,544 bp
  • G to A, chromosome 7 at 27,818,623 bp
  • A to T, chromosome 7 at 27,854,088 bp
  • A to T, chromosome 7 at 27,879,149 bp
  • A to G, chromosome 7 at 143,871,761 bp
  • A to G, chromosome 8 at 13,580,118 bp
  • T to A, chromosome 8 at 48,236,594 bp
  • A to G, chromosome 8 at 61,089,592 bp
  • T to A, chromosome 8 at 71,388,747 bp
  • T to G, chromosome 9 at 21,700,139 bp
  • G to T, chromosome 9 at 22,793,823 bp
  • T to A, chromosome 9 at 38,499,122 bp
  • C to T, chromosome 9 at 49,003,869 bp
  • T to C, chromosome 9 at 66,032,355 bp
  • C to A, chromosome 9 at 122,959,928 bp
  • A to T, chromosome 10 at 130,446,396 bp
  • T to G, chromosome 11 at 42,315,127 bp
  • C to T, chromosome 11 at 65,965,681 bp
  • T to A, chromosome 11 at 70,350,195 bp
  • C to T, chromosome 11 at 105,126,844 bp
  • T to A, chromosome 11 at 109,677,194 bp
  • A to G, chromosome 12 at 105,218,670 bp
  • G to T, chromosome 13 at 23,696,324 bp
  • C to A, chromosome 13 at 63,323,411 bp
  • G to T, chromosome 14 at 121,651,768 bp
  • A to T, chromosome 15 at 8,351,555 bp
  • T to G, chromosome 15 at 16,831,044 bp
  • A to T, chromosome 15 at 27,656,947 bp
  • T to C, chromosome 15 at 55,676,356 bp
  • A to C, chromosome 15 at 82,690,449 bp
  • T to C, chromosome 15 at 88,806,647 bp
  • A to G, chromosome 15 at 100,972,830 bp
  • T to C, chromosome 15 at 102,322,598 bp
  • A to G, chromosome 16 at 32,769,827 bp
  • G to C, chromosome 16 at 34,879,475 bp
  • T to A, chromosome 16 at 88,729,304 bp
  • T to C, chromosome 17 at 3,512,833 bp
  • T to A, chromosome 17 at 27,438,438 bp
  • T to C, chromosome 17 at 27,885,489 bp
  • T to C, chromosome 17 at 38,148,624 bp
  • T to C, chromosome 18 at 47,291,981 bp
  • T to A, chromosome 19 at 8,754,911 bp
  • T to A, chromosome 19 at 8,911,113 bp
  • T to A, chromosome 19 at 12,935,605 bp
  • T to C, chromosome 19 at 18,641,480 bp
  • G to T, chromosome 19 at 24,378,892 bp
  • A to T, chromosome 19 at 26,698,403 bp
  • A to T, chromosome 19 at 27,238,386 bp
  • C to T, chromosome 19 at 47,268,762 bp
  • T to A, chromosome X at 157,372,561 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0726 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038908-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.