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Strain Name:
C57BL/6J-MtgxR6156Btlr/Mmmh
Stock Number:
044303-MU
Citation ID:
RRID:MMRRC_044303-MU
Other Names:
R6156 (G1)
Major Collection:

Strain Information

Ank2
Name: ankyrin 2, brain
Synonyms: ankyrin B, Ank-2, Ankyrin-2, Ankyrin-B, Gm4392
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 109676
HGNC: HGNC:493
Il4i1
Name: interleukin 4 induced 1
Synonyms: Fig1-ps, Fig1, H46, H4, H-46, H-4
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 14204
Homologene: 22567
Rgs11
Name: regulator of G-protein signaling 11
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 50782
HGNC: HGNC:9993
Homologene: 77719
Macf1
Name: microtubule-actin crosslinking factor 1
Synonyms: Acf7, Aclp7, trabeculin alpha
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 11426
Homologene: 136191
Ltbp4
Name: latent transforming growth factor beta binding protein 4
Synonyms: 2310046A13Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 108075
HGNC: HGNC:6717
Homologene: 2645
Thada
Name: thyroid adenoma associated
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 240174
VEGA: 17
Homologene: 75175
Gse1
Name: genetic suppressor element 1, coiled-coil protein
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 382034
Homologene: 40964
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 36,438,826 bp
  • A to T, chromosome 1 at 66,612,250 bp
  • T to A, chromosome 1 at 192,127,842 bp
  • A to G, chromosome 2 at 57,112,352 bp
  • T to A, chromosome 2 at 88,767,590 bp
  • A to T, chromosome 2 at 111,489,750 bp
  • A to T, chromosome 2 at 112,130,100 bp
  • A to G, chromosome 2 at 168,926,168 bp
  • G to T, chromosome 3 at 99,313,115 bp
  • T to C, chromosome 3 at 126,944,237 bp
  • A to G, chromosome 4 at 63,970,352 bp
  • A to G, chromosome 4 at 106,638,583 bp
  • A to G, chromosome 4 at 123,472,280 bp
  • T to A, chromosome 4 at 136,661,505 bp
  • A to C, chromosome 5 at 97,108,269 bp
  • G to A, chromosome 5 at 105,236,149 bp
  • T to C, chromosome 6 at 24,891,438 bp
  • G to T, chromosome 6 at 68,768,623 bp
  • G to C, chromosome 6 at 83,758,133 bp
  • T to C, chromosome 6 at 120,214,840 bp
  • T to A, chromosome 6 at 129,095,098 bp
  • T to C, chromosome 6 at 132,951,492 bp
  • A to G, chromosome 7 at 9,094,612 bp
  • G to T, chromosome 7 at 27,330,162 bp
  • A to G, chromosome 7 at 37,473,856 bp
  • T to C, chromosome 7 at 44,840,184 bp
  • T to C, chromosome 7 at 45,102,060 bp
  • G to T, chromosome 7 at 126,108,844 bp
  • A to G, chromosome 7 at 130,625,764 bp
  • A to G, chromosome 7 at 140,466,845 bp
  • A to G, chromosome 8 at 95,872,268 bp
  • A to G, chromosome 8 at 120,489,127 bp
  • C to G, chromosome 8 at 128,732,054 bp
  • T to A, chromosome 9 at 39,047,375 bp
  • A to T, chromosome 9 at 50,621,152 bp
  • A to T, chromosome 9 at 80,322,824 bp
  • C to T, chromosome 9 at 88,407,339 bp
  • C to A, chromosome 9 at 110,387,781 bp
  • T to C, chromosome 9 at 119,110,213 bp
  • T to C, chromosome 9 at 119,635,583 bp
  • C to T, chromosome 10 at 75,609,326 bp
  • G to A, chromosome 10 at 75,926,491 bp
  • C to T, chromosome 10 at 76,604,170 bp
  • T to C, chromosome 10 at 128,039,291 bp
  • C to A, chromosome 11 at 34,247,789 bp
  • A to T, chromosome 11 at 67,181,053 bp
  • A to G, chromosome 11 at 67,250,792 bp
  • A to T, chromosome 11 at 73,906,621 bp
  • A to G, chromosome 11 at 101,160,809 bp
  • A to G, chromosome 11 at 101,170,187 bp
  • A to G, chromosome 12 at 103,061,458 bp
  • T to C, chromosome 12 at 116,059,906 bp
  • T to C, chromosome 13 at 43,201,038 bp
  • C to T, chromosome 14 at 47,268,196 bp
  • T to C, chromosome 14 at 99,098,779 bp
  • T to C, chromosome 15 at 9,310,676 bp
  • T to G, chromosome 15 at 9,465,834 bp
  • T to C, chromosome 15 at 59,345,399 bp
  • T to G, chromosome 15 at 64,817,639 bp
  • T to C, chromosome 15 at 78,378,813 bp
  • T to C, chromosome 17 at 17,733,344 bp
  • T to C, chromosome 17 at 20,041,647 bp
  • T to A, chromosome 17 at 20,472,185 bp
  • T to A, chromosome 17 at 26,210,465 bp
  • T to C, chromosome 17 at 37,048,427 bp
  • T to A, chromosome 17 at 38,335,173 bp
  • C to T, chromosome 17 at 46,720,641 bp
  • A to G, chromosome 17 at 84,393,367 bp
  • A to G, chromosome 18 at 53,703,223 bp
  • C to A, chromosome 18 at 87,757,107 bp
  • T to C, chromosome 19 at 11,803,510 bp
  • A to G, chromosome 19 at 12,099,120 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R6156 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
044303-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.