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Strain Name:
B6SJL-Tg(Myh6-MYL2*D94A)1Dsc/Mmjax
Stock Number:
075944-JAX
Citation ID:
RRID:MMRRC_075944-JAX
Other Names:
D94A-L1

Strain Information

QUALITY CONTROL OF B6SJL-Tg(Myh6-MYL2*D94A)1Dsc/Mmjax  |  The Jackson Laboratory
Last Updated:
Strain Data and Information Information by Submitter Assessed by MMRRC1,2
Published Provided
Allele-specific genotype3n.d.
Genetic backgroundn.d.
Viability of genotypes available for distributionn.d.
Specific Pathogen- Status n.d.
Recoverability of cryopreserved sperm/embryos4 n.d.
Gene or allele sequence3 n.d.
Gene or allele expression3n.d.
Gene or allele function3n.d.
Observable and/or measurable phenotypesn.d.
Fertility of genotypes available for distributionn.d.
Fecundity/breeding performance n.d.

1 When indicated as verified ("YES"), then please note that information presented is to the best of our knowledge correct and up-to-date at the time of verification at the MMRRC Distribution Center; however, this information is subject to change due to breeding, maintenance, and other actions on the mouse strain at the MMRRC Distribution Center; direct any questions on this table to the MMRRC Distribution Center for this mouse stain.

2 If verification has not been performed (as indicated by "NO"), investigators may request specific verification testing for a fee. Requests should be submitted directly to the MMRRC Distribution Center assigned to the management, archiving, and distribution of the strain. A full listing of available testing and analytical services is available at https://www.mmrrc.org/about/services.php.

3 This information may or may not apply to each individual engineered allele (e.g., Cre, FlpO) present in the strain.

4 Recovery refers to thawing, in vitro fertilization (IVF), and/or embryo culture leading to live offspring.

Myh6
Name: myosin, heavy polypeptide 6, cardiac muscle, alpha
Type: Gene
Species: Rattus norvegicus (norway rat)
Chromosome: 15
MYL2
Name: myosin light chain 2
Type: Gene
Species: Homo sapiens (human)
Chromosome: 12
Tg(Myh6-MYL2*D94A)1Dsc
Name: transgene insertion 1, Danuta Szczesna-Cordary
Type: Transgene
Species: Multi-species
Chromosome: unknown
Alteration at locus: Transgenic
Genetic Alterations
MYL2-D94A transgenic mice harbor human MYL2 cDNA, which carries a D94A mutation, driven by the mouse Myh6 promoter. These mice may be useful when studying familial dilated cardiomyopathy.

Of note, the donating laboratory has made a control MYL2-WT transgenic line available as
ES Cell Line
Not applicable.
Phenotype
Ventricular myosin light chain 2, encoded by the Myl2 (myosin, light polypeptide 2, regulatory, cardiac, slow) gene, is essential in vertebrate smooth muscle contraction and has a regulatory role in striated slow twitch muscle contraction. Mutations in this gene have been associated with hypertrophic and dilated cardiomyopathies. These MYL2-D94A transgenic mice harbor human MYL2 cDNA, which includes an amino acid substitution of aspartic acid to alanine at position 94 (D94A), driven by the mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter.

Hemizygous mice are viable and fertile. The donating laboratory has not evaluated homozygous mice. By 5 months of age, MYL2-D94A male mice demonstrate left ventricular dilation as well as significant reduction in ventricular function (ejection fraction) compared to wildtype transgenic controls. Both male and female MYL2-D94A mice show symptoms of dilated cardiomyopathies by 12 months of age. These MYL2-D94A transgenic mice may be useful when studying familial dilated cardiomyopathy.

Of note, the donating laboratory has made a control MYL2-WT transgenic line available as RRID:MMRRC_075975-JAX.
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.
  • Cardiovascular
  • Models for Human Disease
  • Research Tools
Donor
Danuta Szczesna-cordary, Ph.D., University of Miami Miller School of Medicine.
Primary Reference
Strain Development
The MYL2-D94A transgene was generated using human myosin, light polypeptide 2, regulatory, cardiac, slow (MYL2) cDNA, that contains an amino acid substitution of aspartic acid to alanine at position 94 (D94A), driven by the mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter. The final transgene construct included approximately 5.5kb of the mouse Myh6 promoter, including the first two exons and part of the third, followed by ~498bp of the human MYL2-D94A cDNA and a 630bp 3' untranslated region (UTR) from the human growth hormone (hGH) transcript. The purified linearized DNA was microinjected into the pronuclei of fertilized mouse oocytes obtained from (C57BL/6XSJL)F1 matings. Two founder lines were established, founder line L1 was further expanded. The donating laboratory has indicated that the transgene copy number and integration site are not known. The MYL2-D94A transgenic colony was maintained by crossing hemizygous mice to B6SJLF1/J mice. Upon arrival at The Jackson Laboratory, sperm was cryopreserved. To establish a live colony, an aliquot of frozen sperm was used to fertilize B6SJLF1/J oocytes.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Maintained under virus-free husbandry conditions.
For more information about this colony's health status contact csmmrrc@jax.org
Coat Color
Coat color varies.
Eye
Eye color varies.
MMRRC Breeding System
Backcross
Generation
This strain was established approximately 8 years ago, and to maintain continuity with the original background, we breed this line to non-transgenic B6SJL/F1 mice twice a year (N=16).
Overall Breeding Performance
Good
NOTE: "Hemizygote" as used here refers to males carrying a mutation on the X Chromosome or mice of either sex carrying an inserted transgene with no homologous allele on the other chromosome.
Viability and Fertility: Female Male Comments
Homozygotes are viable: Undetermined Undetermined
Homozygotes are fertile: Undetermined Undetermined
Hetero/Hemizygotes are fertile: Undetermined Undetermined
Age Reproductive Decline: 7 to 9 months 7 to 9 months
Average litter size
10 to 14
Recommended wean age
3 Weeks
Average Pups Weaned
10 to 14

Order Information

Limited quantities of breeder mice (up to 2 males and 2 females or 4 mice) per investigator per month are available from a live colony, usually available to ship in under 12 weeks. Larger quantities may be available, please contact the distributing center directly at csmmrrc@jax.org for more details.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

A Commercial License Agreement from the Donor is required for for-profit entities to use this strain. For more information, please contact Alexandra Vargas.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

A Commercial License Agreement from the Submitter is required for for-profit entities to use this strain. For more information, please contact Alexandra Vargas

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
075944-JAX-HET-F
075944-JAX-HET-M
075944-JAX-WT-F
075944-JAX-WT-M
Heterozygous / Hemizygous Female
Heterozygous / Hemizygous Male
Wild Type Female
Wild Type Male
$229.00 / $229.00
Non-Profit / For-Profit
Per Mouse The csmmrrc@jax.org may assess additional fees for any special requests (e.g., specific age or weight of mice, etc.).
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.