Allele Symbol: Frem2Gt(KST252)Byg
Name: gene trap KST252, BayGenomics
Alteration at locus: Gene Trap
Gene Symbol: Frem2
Name: Fras1 related extracellular matrix protein 2
Alteration at locus: Gene Trap
ES Cell Line: E14Tg2a.4 derived from 129P2/OlaHsd
These mice were not phenotyped prior to cryopreservation, nor were they mated to produce homozygous offspring, therefore the homozygous phenotype is unknown.Mammalian Phenotype Terms:
Strain of Origin: B6;129P2
Strain genetic background: B6;129P2
Strain Development: The ES cell line was injected into C57BL/6J host blastocysts to generate chimeras. The chimeras were then crossed with C57BL/6J mice to determine germline transmission. The resulting heterozygous males were cryoarchived for distribution.
Suggested Control Mice: Wild-type littermates
Donor: MMRRC at University of California, Davis
Jadeja S, Smyth I, Pitera JE, Taylor MS, van Haelst M, Bentley E, McGregor L, Hopkins J, Chalepakis G, Philip N, Perez Aytes A, Watt FM, Darling SM, Jackson I, Woolf AS, Scambler PJ. Identification of a new gene mutated in Fraser syndrome and mouse myelencephalic blebs. Nat Genet. 2005 May;37(5):520-5. Epub 2005 Apr 17. (Medline PMID: 15838507)
*During development at MMRRC at UCD, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the background strain.
Coat color: Agouti/Black
(Note: if sib-mated, it is possible to get pink-eyed chinchilla, the color of the 129P2/Ola, or other mixed variations.)
MMRRC Breeding System: Backcross or Inbreeding
Overall Breeding Performance: Good
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Unknown*||Unknown*|
|Homozygotes are fertile:||Unknown*||Unknown*|
|Heterozygotes are fertile:||Unknown*||Yes|
|Age Reproductive Decline:||Unknown*||36 weeks|
Average litter size: 8
Recommended wean age: 3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.