Strain Detail Sheet

Strain Name    :

B6;129P2-Frem2Gt(KST252)Byg/Mmucd

Stock Number :

010438-UCD

Other Names   :

KST252, B6;129P2-Frem2Gt(KST252)Byg/Mmcd, B6;129P2-XM227142Gt(pGT1TMpfs)1Ucd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Frem2Gt(KST252)Byg
Name: gene trap KST252, BayGenomics
Alteration at locus: Gene Trap
Gene Symbol: Frem2
Name: Fras1 related extracellular matrix protein 2
Chromosome: 3
Alteration at locus: Gene Trap

Genetic Alterations:
This sequence was obtained by 5'RACE PCR from a 129P2/OlaHsd mouse embryonic stem (ES) cell line (E14TG2a.4) with an insertional mutation from gene trap vector pGT1TMpfs,   (Properly spliced).

Genotype Determination:

ES Cell Line: E14Tg2a.4 derived from 129P2/OlaHsd

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

These mice were not phenotyped prior to cryopreservation, nor were they mated to produce homozygous offspring, therefore the homozygous phenotype is unknown.

Strain of Origin: B6;129P2

Strain genetic background: B6;129P2

Strain Development: The ES cell line was injected into C57BL/6J host blastocysts to generate chimeras.  The chimeras were then crossed with C57BL/6J mice to determine germline transmission.  The resulting heterozygous males were cryoarchived for distribution.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Research Tools

Strain Origin

Donor: MMRRC at University of California, Davis

Primary Reference:

Jadeja S, Smyth I, Pitera JE, Taylor MS, van Haelst M, Bentley E, McGregor L, Hopkins J, Chalepakis G, Philip N, Perez Aytes A, Watt FM, Darling SM, Jackson I, Woolf AS, Scambler PJ. Identification of a new gene mutated in Fraser syndrome and mouse myelencephalic blebs. Nat Genet. 2005 May;37(5):520-5. Epub 2005 Apr 17. (Medline PMID: 15838507)

Colony and Husbandry Information

Special Considerations

*During development at MMRRC at UCD, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the background strain.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Appearance

Coat color: Agouti/Black
(Note: if sib-mated, it is possible to get pink-eyed chinchilla, the color of the 129P2/Ola, or other mixed variations.)

Other:

Breeding

MMRRC Breeding System: Backcross or Inbreeding

Breeding Scheme(s):

  • C57BL/6J female x Heterozygous male or reciprocal
  • Wild-type female x Heterozygous male sibling or reciprocal

Generation: N2

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Unknown* Unknown*
Homozygotes are fertile: Unknown* Unknown*
Heterozygotes are fertile: Unknown* Yes
 
Age Reproductive Decline: Unknown* 36 weeks

Average litter size: 8

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
010438-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
010438-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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