Strain Detail Sheet

Strain Name    :

STOCK Tg(Foxa3-cre)1Khk/Mmmh

Stock Number :

011121-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Transgene: Tg(Foxa3-cre)1Khk
Name: transgene insertion 1, Klaus H Kaestner
Alteration at locus: Transgenic (cre)
Transgene: cre (Bacteriophage P1)
Name: cre recombinase
Alteration at locus: Transgenic (cre)
Promoter: Foxa3
Name: forkhead box A3
Alteration at locus: Transgenic (cre)

Genetic Alterations:
Cre was targeted into exon 2 of the Foxa3 gene in a 170 kb YAC. Transgenic mouse with single copy insertion obtained, expression profile confirmed.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: None
Hemizygous phenotype: None, expression of cre

Strain of Origin: Mixed

Strain genetic background: CD1

Strain Development: Transgenic line was made by pronuclear injection into B6/D2 F2 hybrid fertilized oocytes and has since been bred to CD1 outbred mice.

Suggested Control Mice:

  • Wildtype littermates

Research Applications

  • Apoptosis
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Diabetes/Obesity
  • Endocrine Deficiency
  • Internal/Organ
  • Metabolism
  • Models for Human Disease
  • Research Tools

Strain Origin

Donor: Klaus H. Kaestner, Ph.D., University of Pennsylvania

Primary Reference:

  • Lee CS, Sund NJ, Behr R, Herrera PL, Kaestner KH. Foxa2 is required for the differentiation of pancreatic alpha-CELL. Dev Biol. 2005 Feb 15;278(2):484-95. (Medline PMID: 15680365)
  • Katz JP, Perreault N, Goldstein BG, Actman L, McNally SR, Silberg DG, Furth EE, Kaestner KH. Loss of Klf4 in mice causes altered proliferation and differentiation and precancerous changes in the adult stomach. Gastroenterology. 2005 Apr;128(4):935-45. (Medline PMID: 15825076)
  • Lee CS, Friedman JR, Fulmer JT, Kaestner KH. The initiation of liver development is dependent on Foxa transcription factors. Nature. 2005 Jun 16;435(7044):944-7. (Medline PMID: 15959514 )

Colony and Husbandry Information

Special Considerations

The recommended breeding scheme is to maintain the outbred CD-1 background by mating transgene positive hemizygous carriers to CD-1 stock mice. This transgene has never been characterized in the homozygous state but mating of hemizygous carriers to hemizygous carriers has resulted in a percentage of offspring with seizures and sudden death. This phenotype maybe a consequence of the transgene being homozygous or an unrelated mutation. To avoid this seizure phenotype it is highly recommended that the transgene be maintained in the hemizygous state by mating carrier animals to vendor purchased CD-1 outbred stock mice.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
011121-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
011121-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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Generated: 04/17/2014