Strain Name:
STOCK Tg(Foxa3-cre)1Khk/Mmmh
Stock Number:
011121-MU
Citation ID:
RRID:MMRRC_011121-MU

Gene Information

Transgene: cre (Enterobacteria phage P1 (bacteriophage P1))
Name: cre recombinase
Alteration at locus: Transgenic (cre)
Monarch Initiative (Disease and Phenotype Associations):
Promoter: Foxa3 (Mus musculus (mouse))
Name: forkhead box A3
Synonyms: Tcf-3g, Hnf3g, Hnf-3g, Tcf3g
Chromosome: 7
Alteration at locus: Transgenic (cre)
Monarch Initiative (Disease and Phenotype Associations): MGI:1347477
Transgene: Tg(Foxa3-cre)1Khk (Mus musculus (mouse))
Name: transgene insertion 1, Klaus H Kaestner; transgene insertion 1, Klaus H Kaestner
Synonyms: Foxa3.cre, Foxa3Cre, Foxa3cre, Tg(Foxa3-cre)<1Khk>, Foxa3-Cre
Chromosome: unknown
Alteration at locus: Transgenic (cre)
Monarch Initiative (Disease and Phenotype Associations): MGI:2664968
Genetic Alterations
Cre was targeted into exon 2 of the Foxa3 gene in a 170 kb YAC. Transgenic mouse with single copy insertion obtained, expression profile confirmed.
Genotype Determination
ES Cell Line
N/A
Phenotype
Homozygous: same as wild-type 
Hemizygous: None, expression of cre
MeSH Terms
  • Animals
  • Base Sequence
  • Cell Differentiation
  • Chromosomes, Artificial, Yeast
  • DNA Primers
  • DNA-Binding Proteins/deficiency
  • DNA-Binding Proteins/genetics
  • Embryonic Development/genetics
  • Gene Deletion
  • Gene Expression Regulation, Developmental
  • Genotype
  • Hepatocyte Nuclear Factor 3-beta
  • Hypoglycemia/genetics
  • Islets of Langerhans/embryology
  • Mice
  • Mice, Knockout
  • Nuclear Proteins/deficiency
  • Nuclear Proteins/genetics
  • Pancreas/embryology
  • Reproducibility of Results
  • Transcription Factors/deficiency
  • Transcription Factors/genetics
  • CDX2 Transcription Factor
  • Cell Cycle Proteins/metabolism
  • Cell Proliferation
  • Cyclin-Dependent Kinase Inhibitor p21
  • DNA-Binding Proteins/metabolism
  • Gastric Mucosa/pathology
  • Homeodomain Proteins/metabolism
  • Homeostasis
  • Kruppel-Like Transcription Factors
  • Mutation
  • Precancerous Conditions/genetics
  • Precancerous Conditions/metabolism
  • Precancerous Conditions/pathology
  • Stomach/pathology
  • Stomach Neoplasms/metabolism
  • Tissue Distribution
  • Transcription Factors/metabolism
  • Trefoil Factor-2
  • Fibroblast Growth Factor 2/pharmacology
  • Gene Expression Regulation, Developmental/drug effects
  • Hepatocyte Nuclear Factor 3-alpha
  • Hepatocyte Nuclear Factor 3-gamma
  • Liver/drug effects
  • Liver/embryology
  • Liver/metabolism
  • Nuclear Proteins/metabolism
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
Strain Development
Transgenic line was made by pronuclear injection into B6/D2 F2 hybrid fertilized oocytes and has since been bred to CD1 outbred mice.
Suggested Control Mice
  • Wildtype littermates
  • Apoptosis
  • Cancer
  • Cell Biology
  • Developmental Biology
  • Diabetes/Obesity
  • Endocrine Deficiency
  • Internal/Organ
  • Metabolism
  • Models for Human Disease
  • Research Tools
Donor
Klaus H. Kaestner, Ph.D., Perelman School of Medicine at the University of Pennsylvania
Primary Reference
  • Lee CS, Sund NJ, Behr R, Herrera PL, Kaestner KH. Foxa2 is required for the differentiation of pancreatic alpha-CELL. Dev Biol. 2005 Feb 15;278(2):484-95. (Medline PMID: 15680365)
  • Katz JP, Perreault N, Goldstein BG, Actman L, McNally SR, Silberg DG, Furth EE, Kaestner KH. Loss of Klf4 in mice causes altered proliferation and differentiation and precancerous changes in the adult stomach. Gastroenterology. 2005 Apr;128(4):935-45. (Medline PMID: 15825076)
  • Lee CS, Friedman JR, Fulmer JT, Kaestner KH. The initiation of liver development is dependent on Foxa transcription factors. Nature. 2005 Jun 16;435(7044):944-7. (Medline PMID: 15959514)

Colony and Husbandry Informationon

The recommended breeding scheme is to maintain the outbred CD-1 background by mating transgene positive hemizygous carriers to CD-1 stock mice. This transgene has never been characterized in the homozygous state but mating of hemizygous carriers to hemizygous carriers has resulted in a percentage of offspring with seizures and sudden death. This phenotype maybe a consequence of the transgene being homozygous or an unrelated mutation. To avoid this seizure phenotype it is highly recommended that the transgene be maintained in the hemizygous state by mating carrier animals to vendor purchased CD-1 outbred stock mice.

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
011121-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity2
011121-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.