Strain Name    : C57BL/6J-Mbtps1wrt/Mmucd

Stock Number : 012829-UCD

Other Names   : woodrat, C57BL/6-Mbtps1wrt/Mmcd

Gene Information

(provided by MGI)
Allele Symbol: Mbtps1wrt
Name: woodrat
Chromosome: 8
Alteration at locus: Chemically Induced
Gene Symbol: Mbtps1
Name: membrane-bound transcription factor peptidase, site 1
Chromosome: 8
Alteration at locus: Chemically Induced
Genetic Alterations:
486A->G, Y501C

Genotype Determination:

ES Cell Line: Not applicable

Additional mutations found on this strain:

AlleleSourceChrCoordTypePredicted EffectPPH Score
R0194:Mbtps1 UTSW 8 119535369 missense probably damaging 1.00
R0270:Mbtps1 UTSW 8 119538117 splice donor site probably benign
R0485:Mbtps1 UTSW 8 119522601 splice acceptor site probably benign
R1269:Mbtps1 UTSW 8 119520277 missense probably damaging 1.00
R1351:Mbtps1 UTSW 8 119518162 missense possibly damaging 0.95
R1536:Mbtps1 UTSW 8 119546125 missense probably benign 0.01
R1542:Mbtps1 UTSW 8 119546247 splice acceptor site probably null
R1543:Mbtps1 UTSW 8 119542069 splice donor site
R1580:Mbtps1 UTSW 8 119538900 missense possibly damaging 0.79
R1587:Mbtps1 UTSW 8 119518219 missense probably damaging 0.96
R1715:Mbtps1 UTSW 8 119542730 missense probably benign 0.40
R1845:Mbtps1 UTSW 8 119522493 missense probably benign 0.13
R1956:Mbtps1 UTSW 8 119524415 splice donor site
R1958:Mbtps1 UTSW 8 119524415 splice donor site
R2147:Mbtps1 UTSW 8 119538859 missense probably benign 0.01
R2157:Mbtps1 UTSW 8 119542727 missense probably benign 0.01
R2416:Mbtps1 UTSW 8 119538917 missense probably damaging 1.00
R2910:Mbtps1 UTSW 8 119546037 missense possibly damaging 0.82
R2911:Mbtps1 UTSW 8 119546037 missense possibly damaging 0.82
R3079:Mbtps1 UTSW 8 119531205 missense probably benign 0.40
R3079:Mbtps1 UTSW 8 119538863 missense probably damaging 1.00
R3080:Mbtps1 UTSW 8 119531205 missense probably benign 0.40
R3080:Mbtps1 UTSW 8 119538863 missense probably damaging 1.00
R4116:Mbtps1 UTSW 8 119541652 missense probably benign 0.00
R4296:Mbtps1 UTSW 8 119522499 missense not run
X0017:Mbtps1 UTSW 8 119531124 missense probably damaging 1.00
X0027:Mbtps1 UTSW 8 119522547 missense probably damaging 1.00

Phenotype:

Homozygous phenotype: The mutant mice show progressive hypopigmentation of the fur (grey), with intra- and inter-hair variegation. The blood cholesterol level is decreased by 75 percent as compared to wild-type values. The mice are sensitive to mouse cytomegalovirus (MCMV) infections. The killing function of cytotoxic T cells (CTL) is impaired.

Heterozygous phenotype: The heterozygous mice appear to be wild-type for the phenotypes tested by donor.

Mammalian Phenotype Terms:
Allelic Composition: Mbtps1wrt/Mbtps1wrt (Genetic Background: C57BL/6J-Mbtps1wrt )

Allelic Composition: Mbtps1Gt(PST115)Byg/Mbtps1wrt (Genetic Background: involves: 129P2/OlaHsd * C57BL/6 )

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: The woodrat mutation is recessive. It was first isolated as a homozygous G3 male. This original mouse was bred to C57BL/6 females, and the second generation was backcrossed to the mutant founder. The woodrat homozygous mutant females have low fertility, thus the stock is currently maintained as homozygous male bred with heterozygous females.

Suggested Control Mice: Wild-type littermates

  • Cardiovascular
  • Cell Biology
  • Immunology and Inflammation
  • Metabolism
  • Research Tools
  • Virology

Donor: Bruce Beutler, M.D., The Scripps Research Institute

Primary Reference:

  • Brandl K; Rutschmann S; Li X; Du X; Xiao N; Schnabl B; Brenner DA; Beutler B, Enhanced sensitivity to DSS colitis caused by a hypomorphic Mbtps1 mutation disrupting the ATF6-driven unfolded protein response., Proc Natl Acad Sci U S A 2009 Mar 3;106(9):3300-5 (Medline PMID: 19202076)
  • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
012829-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
012829-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
012829-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.