Strain Detail Sheet

Strain Name    :

C57BL/6J-Mbtps1wrt/Mmucd

Stock Number :

012829-UCD

Other Names   :

woodrat, C57BL/6-Mbtps1wrt/Mmcd

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Mbtps1wrt
Name: woodrat
Alteration at locus: Chemically Induced
Gene Symbol: Mbtps1
Name: membrane-bound transcription factor peptidase, site 1
Chromosome: 8
Alteration at locus: Chemically Induced

Genetic Alterations:
486A->G, Y501C

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: The mutant mice show progressive hypopigmentation of the fur (grey), with intra- and inter-hair variegation. The blood cholesterol level is decreased by 75 percent as compared to wild-type values. The mice are sensitive to mouse cytomegalovirus (MCMV) infections. The killing function of cytotoxic T cells (CTL) is impaired.

Heterozygous phenotype: The heterozygous mice appear to be wild-type for the phenotypes tested by donor.


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype
Mbtps1wrt/Mbtps1wrt
        C57BL/6J-Mbtps1<wrt>
  • mortality/aging
    • increased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:146454)
      • over 90% of mice die within 30 hours of injection with tunicamycin (an endoplasmic reticulum stressor) compared to under 10% for controls
  • pigmentation phenotype
    • abnormal coat/hair pigmentation (MGI Ref IDs J:146454, J:123123)
      • (MGI Ref ID J:146454)
      • in adults, the coat is of a homogeneous color and made of hair with a white base and a grey/brown tip (MGI Ref ID J:123123)
    • hypopigmentation (MGI Ref ID J:146454)
    • hypopigmentation (MGI Ref IDs J:94077, J:123123)
      • homozygous mice exhibit a modest hypopigmentation 8 days post-partum that progresses to premature grayness (MGI Ref ID J:123123)
      • the mutant mice show progressive hypopigmentation of the fur (grey), with intra- and inter-hair variegation (MGI Ref ID J:94077)
  • reproductive system phenotype
    • reduced female fertility (MGI Ref ID J:123123)
      • homozygous mutant females are sterile when bred with homozygous males, and crosses with heterozygous males only give birth to heterozygous pups
  • homeostasis/metabolism phenotype
    • decreased circulating cholesterol level (MGI Ref IDs J:94077, J:123123)
      • homozygous mice have significantly reduced levels of serum cholesterol (MGI Ref ID J:123123)
      • mice have normal levels of triglycerides (MGI Ref ID J:123123)
      • in homozygotes, the blood cholesterol level is decreased by 75 percent as compared to wild-type values (MGI Ref ID J:94077)
      • decreased circulating HDL cholesterol level (MGI Ref ID J:123123)
        • homozygous mice have significantly reduced levels of HDL
    • increased circulating LDL cholesterol level (MGI Ref ID J:123123)
      • homozygous mice have elevated levels of LDL
    • increased sensitivity to xenobiotic induced morbidity/mortality (MGI Ref ID J:146454)
      • over 90% of mice die within 30 hours of injection with tunicamycin (an endoplasmic reticulum stressor) compared to under 10% for controls
  • immune system phenotype
    • decreased susceptibility to bacterial infection (MGI Ref ID J:123123)
      • homozygotes are resistant to Listeria infections
    • defective cytotoxic T cell cytolysis (MGI Ref ID J:94077)
      • in homozygotes, the killing function of cytotoxic T cells (CTL) is impaired
    • increased interleukin-1 beta secretion (MGI Ref ID J:146454)
      • colon explants from dextran sulfide sodium (DSS) treated mice produce more IL-1beta
    • increased interleukin-6 secretion (MGI Ref ID J:146454)
      • colon explants produce more IL-6 than controls
    • increased susceptibility to induced colitis (MGI Ref ID J:146454)
      • mice have an exaggerated colitic response to 2% dextran sulfide sodium (DSS) treatment of drinking water
      • starting with 4 days of DSS treatment, mice lose significantly more weight than controls
      • by 7 days of DSS treatment, mice have lost almost 20% of their initial weight compared to 10% for controls
      • after 9 days of DSS treatment, colons are thickened and shortened with bleeding visible
      • histological analysis reveals dramatic leukocyte recruitment and crypt loss after 7 days of DSS treatment
      • colon explants from DSS treated mice produce more IL-1beta
      • bone marrow chimera experiments indicate non-hematopoietic cells expressing the mutant allele increases susceptibility to induced colitis
      • tunicamycin injection causes a colitis-like disease in mutant mice but not in control mice
    • increased susceptibility to viral infection (MGI Ref IDs J:94077, J:123123)
      • homozygotes are susceptible to MCMV infections (MGI Ref ID J:123123)
      • mice present reduced levels of gamma interferon but elevated levels of interleukine 6, 12 and type 1 interferon 36 hours post infection (MGI Ref ID J:123123)
      • mice are sensitive to mouse cytomegalovirus (MCMV) infections (MGI Ref ID J:94077)
  • cellular phenotype
    • abnormal cell physiology (MGI Ref ID J:123123)
      • expression of ATF6-dependent unfolded protein response genes is reduced in homozygous mutants upon activation of the endoplasmic reticulum stress
  • digestive/alimentary phenotype
    • increased susceptibility to induced colitis (MGI Ref ID J:146454)
      • mice have an exaggerated colitic response to 2% dextran sulfide sodium (DSS) treatment of drinking water
      • starting with 4 days of DSS treatment, mice lose significantly more weight than controls
      • by 7 days of DSS treatment, mice have lost almost 20% of their initial weight compared to 10% for controls
      • after 9 days of DSS treatment, colons are thickened and shortened with bleeding visible
      • histological analysis reveals dramatic leukocyte recruitment and crypt loss after 7 days of DSS treatment
      • colon explants from DSS treated mice produce more IL-1beta
      • bone marrow chimera experiments indicate non-hematopoietic cells expressing the mutant allele increases susceptibility to induced colitis
      • tunicamycin injection causes a colitis-like disease in mutant mice but not in control mice
  • integument phenotype
    • abnormal coat/hair pigmentation (MGI Ref IDs J:146454, J:123123)
      • (MGI Ref ID J:146454)
      • in adults, the coat is of a homogeneous color and made of hair with a white base and a grey/brown tip (MGI Ref ID J:123123)

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: The woodrat mutation is recessive. It was first isolated as a homozygous G3 male. This original mouse was bred to C57BL/6 females, and the second generation was backcrossed to the mutant founder. The woodrat homozygous mutant females have low fertility, thus the stock is currently maintained as homozygous male bred with heterozygous females.

Suggested Control Mice: Wild-type littermates

Research Applications

  • Cardiovascular
  • Cell Biology
  • Immunology and Inflammation
  • Metabolism
  • Research Tools
  • Virology

Strain Origin

Donor: Bruce Beutler, M.D., The Scripps Research Institute

Primary Reference:

  • Brandl K; Rutschmann S; Li X; Du X; Xiao N; Schnabl B; Brenner DA; Beutler B, Enhanced sensitivity to DSS colitis caused by a hypomorphic Mbtps1 mutation disrupting the ATF6-driven unfolded protein response., Proc Natl Acad Sci U S A 2009 Mar 3;106(9):3300-5 (Medline PMID: 19202076)
  • For additional information see: Mutagenetix, a catalog of mutations identified in the Beutler Laboratory at The Scripps Research Institute.

Colony and Husbandry Information

Special Considerations

None

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu.

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
012829-UCD-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
012829-UCD-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2
012829-UCD-EMBRYOCryo-preserved embryos
$1,038.00
Non-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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