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Strain Detail Sheet

Published: 06/16/2019
Revised: 04/27/2020

Strain Name:
S15/JlsMmmh
Stock Number:
015111-MU
Citation ID:
RRID:MMRRC_015111-MU

Strain Information

Gene Details

Genetic Alterations
Strain S15 was selected long term for large liter size, and then inbred with continued selection for large litter size. Strain S15 is a good maternal strain, although its lactational yields / pup weight gains are only average. Nevertheless S15 tends to more readily accept fostered pups without cannibalizing them than CD-1 and CD-1 derived inbred dams.
Genotype Determination

Strain Description

Phenotype
Homozygous: The spontaneous ovulation rate of adult S15 females (16.7 + 0.4 eggs) was not different from that of line S1 (17.6 + 0.5 eggs) (Spearow and Bradford 1983). In contrast, the ovulation rate of S15 strain mice were greater than that of S12 (15.7 + 0.2 eggs), Control inbred strain C8 (10.4 + 0.4) and the outbred control. line C (10.3 + 0.3 eggs).

The physiological mechanisms by which selection for large litter size in line S1 increased spontaneous ovulation rate over that of the randomly selected controls (Line C) involved changes in regulation of serum gonadotropins, sensitivity to estrogen, ovarian follicle populations, kinetics of follicular growth, induction of follicle maturation by gonadotropins, induction of LH receptors by gonadotropins, and induction of ovarian steroidogenesis (Spearow et al. 1983; Spearow 1985; Spearow 1986; Spearow et al. 1987). The number of LH receptors induced by a DES treatment for 4 days and FSH for 2 days was significantly lower per ovary and per ug granulosa cell DNA in S15 and other S1 derived inbreds and crosses, than in Line C, or C derived inbreds (Spearow et al. 1983).

In a survey of 16 inbred strains, gonadotropin-induced ovulation rate (ORI) differed 6 fold with ORI ranging from 9 eggs in A/J and AKR/J to 54 in C57BL/6J. S15 was intermediate, further demonstrating that the increased ovulation rate of this strain was not simply due to increased ovarian response to gonadotropins (Spearow 1988). The gonadotropin induced ovulation rate and aromatase activity of S15 X A/J is much greater than that of A/J, but less than that of C57BL/6J. S15 was used as maternal strain in mapping ORI QTL in S15XA.B6 congenic testcrosses (Spearow et al. 1997; Spearow et al. 1998; Spearow et al. In Preparation). Recent evaluations on day 15-16 of gestation, showed that the number of normal fetuses averaged 4.8 + 0.5 in CN1, 12.4 + 0.5 in S15/Jls, and 12.5 + 0.5 in the CD-1 derived inbred strain, CD10/Jls (P<0.0001) (Spearow, et al in preparation).

Heterozygous: The S15 X C8 F1 averaged 12.7 + 0.7 eggs, which is not significantly different than the midparental average of 13.55 eggs.
MeSH Terms
  • Animals
  • Chorionic Gonadotropin/pharmacology
  • Female
  • Genetic Variation
  • Gonadotropins/pharmacology
  • Gonadotropins, Equine/pharmacology
  • Mice
  • Ovulation/drug effects
  • Reproduction/drug effects
  • Selection, Genetic
  • Genes
  • Inbreeding
  • Litter Size
  • Male
  • Pregnancy
  • Body Weight
  • Crosses, Genetic
  • Diethylstilbestrol/pharmacology
  • Follicle Stimulating Hormone/pharmacology
  • Mice, Inbred Strains/genetics
  • Ovulation
  • Receptors, Cell Surface/drug effects
  • Receptors, Cell Surface/genetics
  • Receptors, LH
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Reproduction

Strain Origin

Submitter
Jimmy L. Spearow, Ph.D., Univeristy of California at Davis
Primary Reference

Bradford GE. Selection for litter size in mice in the presence and absence of gonadotropin treatment. Genetics. 1968 Feb;58(2):283-95. (Medline PMID: 5664389)

Eklund J, Bradford GE. Genetic analysis of a strain of mice plateaued for litter size. Genetics. 1977 Mar;85(3):529-42. (Medline PMID: 558934)

Spearow JL, Bradford GE. Genetic variation in spontaneous ovulation rate and LH receptor induction in mice. J Reprod Fertil. 1983 Nov;69(2):529-37. (Medline PMID: 6313917)

Strain Development
Dr G. Eric Bradford started from a 4-way cross of C57BL/6J. AKR/J, C3H/J, and DBA/2J, (obtained via Simonsen Laboratories). He selected randomly in the control Line C, and selected for large litter size in Line S1 {Bradford, 1968 #11}. Starting from Line C, Bradford also selected for small litter size in Line CN- {Bradford, 1979 #2940}.

Long term selection markedly increased litter size mainly by increasing ovulation rate {Bradford, 1968 #11}. Selection for small litter size decreased litter size by decreasing embryo survival {Bradford, 1979 #2940}. After 34 generations of selection for large litter size in Line S1, inbreeding was initiated with continued selection for large litter size {Eklund, 1977 #472} to produce large litter size selected inbred strains S15 and S12. Only strain S15/Jls remains and is currently at generation 104 of inbreeding.

Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*		 Units Notes
015111-MU-EMBRYO Cryo-preserved embryos $1,038.00 / $2,621.00
Non-Profit / For-Profit 		Aliquot Approximate quantity2 : 20-40 embryos / aliquot
015111-MU-SPERM Cryo-preserved spermatozoa $546.25 / $1,021.25
Non-Profit / For-Profit 		Aliquot Approximate quantity3
015111-MU-RESUS Litter recovered from cryo-archive $2,016.00 / $4,103.00
Non-Profit / For-Profit 		Litter Recovered litter4; additional fees for any special requests.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.