Strain Name:
B6.129S6(FVB)-Rictortm1.3Mgn/Mmucd
Stock Number:
015200-UCD
Citation ID:
RRID:MMRRC_015200-UCD
Other Names:
pia[lacZ], B6.129S6(FVB)-Rictortm1.3Mgn/Mmcd

Gene Information

Allele Symbol: Rictortm1.3Mgn (Mus musculus (mouse))
Name: RPTOR independent companion of MTOR, complex 2; targeted mutation 1.3, Mark A Magnuson
Synonyms: ric
Chromosome: 15
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations): MGI:3703325
Gene Symbol: Rictor (Mus musculus (mouse))
Name: RPTOR independent companion of MTOR, complex 2
Synonyms: 4921505C17Rik, 6030405M08Rik, D530039E11Rik
Chromosome: 15
Alteration at locus: Knock-In
Monarch Initiative (Disease and Phenotype Associations): MGI:1926007
Genetic Alterations
This knock-in (null) allele resulted from a multi-allelic gene targeting strategy that involved the use of both FRT and loxP sites. This pia[lacZ] allele contains a lacZ-pA region flanked on the 5' side by a single (remnant) FRT site and one the 3' side by a single loxP site.
Genotype Determination
ES Cell Line
TL1/TL-1 derived from 129S6/SvEvTac
Phenotype
Homozygous: Homozygous exhibit an embryonic lethal phenotype. Embryos exhibit growth arrest after E9.5 and die by E11.5.

Heterozygous: Heterozyous animals are viable and do not exhibit any obvious mutant phenotype.
Mammalian Phenotype Terms
Allelic Composition: Rictortm1.3Mgn/Rictortm1.3Mgn (Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * FVB/N )

Allelic Composition: Rictortm1.2Mgn/Rictortm1.3Mgn (Genetic Background: involves: 129S6/SvEvTac * C57BL/6 * FVB/N * SJL )

MeSH Terms
  • Actins/metabolism
  • Alleles
  • Animals
  • Carrier Proteins/metabolism
  • Cell Proliferation
  • Cells, Cultured
  • Embryo, Mammalian/cytology
  • Fetal Development/genetics
  • Fetal Viability/genetics
  • Fibroblasts/metabolism
  • Fibroblasts/physiology
  • Gene Targeting
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Molecular Sequence Data
  • Protein Kinases/analysis
  • Protein Kinases/metabolism
  • Proto-Oncogene Proteins c-akt/metabolism
  • Rapamycin-Insensitive Companion of mTOR Protein
  • TOR Serine-Threonine Kinases
Strain Development
A multiallelic gene targeting was developed that was used to generate a pia[lacZ+neo] allele in mouse embryonic stem cells. After germline transmission a neoR cassette and exon 3 (which were flanked by loxP sites) were removed by mating to EIIa- Cre transgenic mice, thereby generating mice containing the pia[lacZ] allele. Pia[lacZ} mice were subsequently backcrossed into a C57BL/6J background.
Suggested Control Mice
  • Wildtype littermates
  • Apoptosis
  • Cancer
  • Cardiovascular
  • Cell Biology
  • Dermatology
  • Developmental Biology
  • Diabetes/Obesity
  • Endocrine Deficiency
  • Hematology
  • Immunology and Inflammation
  • Internal/Organ
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Reproduction
  • Research Tools
  • Sensorineural
Donor
Mark A. Magnuson, M.D., Vanderbilt University
Primary Reference
Shiota C, Woo JT, Lindner J, Shelton KD, Magnuson MA. Multiallelic disruption of the rictor gene in mice reveals that mTOR complex 2 is essential for fetal growth and viability. Dev Cell. 2006 Oct;11(4):583-9. Epub 2006 Sep 7. (Medline PMID: 16962829)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@ucdavis.edu .

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
015200-UCD-EMBRYO Cryo-preserved embryos $1,038.00 / $1,549.67
Non-Profit / For-Profit
Aliquot Approximate quantity3 : 20-40 embryos / aliquot
015200-UCD-SPERM Cryo-preserved spermatozoa $546.25 / $871.90
Non-Profit / For-Profit
Aliquot Approximate quantity2
015200-UCD-RESUS Litter recovered from cryo-archive $3,033.00 / $6,017.01
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.