Strain Name:
B6.129S4-Trp53tm1Adv/Mmmh
Stock Number:
016832-MU
Citation ID:
RRID:MMRRC_016832-MU
Major Collection:

Strain Information

Trp53tm1Adv
Name: transformation related protein 53; targeted mutation 1, Annemieke de Vries
Synonyms: p53.S389A
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 11
Alteration at locus: Knock-In
Trp53
Name: transformation related protein 53
Synonyms: p53, p44
Type: Gene
Species: Mouse
Chromosome: 11
Alteration at locus: Knock-In
NCBI: 22059
Homologene: 460
Genetic Alterations
The targeting vector harboring the p53.S389A mutation was created by site-directed mutagenesis (Stratagene) and introduced into the mouse genome by homologous recombination in ES cells. The selectable neomycin (NEO) marker, preceded by transcription stops, flanked by loxP sites, was present in intron 11. The targeting vectors were also fitted with a diphtheria toxin A (DTA) expression cassette.
Genotype Determination
ES Cell Line
J1 derived from 129S4/SvJae
Phenotype
Homozygous: Homozygous p53. S389A mice are born at Medelian ratios, are viable and fertile and have no obvious phenotypic abnormalities and show normal survival. p53.S389A mice are cancer prone upon exposure to UV and 2-AAF, whereas gamma- irradiation does not result in an increased cancer susceptibility.

Heterozygous: Not characterized.
Mammalian Phenotype Terms
Allelic Composition: Trp53tm1Adv/Trp53tm1Adv (Genetic Background: involves: 129S4/SvJae * C57BL/6 )

Allelic Composition: Trp53tm1Adv/Trp53+ (Genetic Background: involves: 129S4/SvJae * C57BL/6 )

MeSH Terms
  • Animals
  • Antibiotics, Antineoplastic/pharmacology
  • Apoptosis/physiology
  • Carcinoma/pathology
  • Cell Cycle/physiology
  • Cells, Cultured
  • Doxorubicin/pharmacology
  • Fibroblasts/cytology
  • Fibroblasts/physiology
  • Gamma Rays
  • Gene Expression Regulation/radiation effects
  • Humans
  • Mice
  • Neoplasms, Squamous Cell/pathology
  • Papilloma/pathology
  • Phenotype
  • Phosphorylation
  • Point Mutation
  • Serine/metabolism
  • Skin/radiation effects
  • Stem Cells/physiology
  • Survival Rate
  • Thymus Gland/cytology
  • Thymus Gland/drug effects
  • Transcriptional Activation
  • Tumor Suppressor Protein p53/genetics
  • Tumor Suppressor Protein p53/metabolism
  • Ultraviolet Rays
  • 2-Acetylaminofluorene/toxicity
  • Carcinogens/toxicity
  • DNA Damage
  • Female
  • Genes, p53/radiation effects
  • Lymphoma/etiology
  • Lymphoma/genetics
  • Lymphoma/metabolism
  • Male
  • Mice, Inbred C57BL
  • Mutation
  • Neoplasms, Radiation-Induced/genetics
  • Neoplasms, Radiation-Induced/metabolism
  • Skin Neoplasms/etiology
  • Skin Neoplasms/genetics
  • Skin Neoplasms/metabolism
  • Ultraviolet Rays/adverse effects
  • Urinary Bladder Neoplasms/chemically induced
  • Urinary Bladder Neoplasms/genetics
  • Urinary Bladder Neoplasms/metabolism
Strain Development
Original strain contained pGK-neo cassette flanked by loxP sites. Selectable marker was removed by crossing with Cre-deleter (Schwenk et al, 1995, Nucl Acids Res 23, 5080-5081).
All strains from this donor were crossed with oubred C57BL/6 strains every two years.
Suggested Control Mice
  • Wildtype littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
  • Apoptosis
  • Cancer
  • Research Tools
Donor
Annemieke de Vries, Ph.D., Ntl. Inst. for Public Health & Environmnent (RIVM)
Primary Reference
  • Bruins W, Zwart E, Attardi LD, Iwakuma T, Hoogervorst EM, Beems RB, Miranda B, van Oostrom CT, van den Berg J, van den Aardweg GJ, Lozano G, van Steeg H, Jacks T, de Vries A. Increased sensitivity to UV radiation in mice with a p53 point mutation at Ser389. Mol Cell Biol. 2004 Oct;24(20):8884-94. (Medline PMID: 15456863)
  • Hoogervorst EM, Bruins W, Zwart E, van Oostrom CT, van den Aardweg GJ, Beems RB, van den Berg J, Jacks T, van Steeg H, de Vries A. Lack of p53 Ser389 phosphorylation predisposes mice to develop 2-acetylaminofluorene-induced bladder tumors but not ionizing radiation-induced lymphomas. Cancer Res. 2005 May 1;65(9):3610-6. (Medline PMID: 15867355)

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
016832-MU-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
016832-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.