Strain Detail Sheet

Strain Name    :

Stock Number :

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Sgcdtm189Kcam
Name: sarcoglycan, delta (dystrophin-associated glycoprotein) / (unnamed provisional allele)
Alteration at locus: Knockout
Gene Symbol: Sgcd
Name: sarcoglycan, delta (dystrophin-associated glycoprotein)
Chromosome: 11
Alteration at locus: Knockout

Genetic Alterations:
A targeting vector containing a neo cassette was inserted to replace exon 2, which encodes for 63 amino acids of the intracelular domain and the entire transmembrane region.

Genotype Determination:

ES Cell Line: R1

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous Phenotype: Sgcd null mice show a phenotype consistent with human limb girdle muscular dystrophy. Pathology includes large regions of necrosis in nearly all skeletal muscles. Severe necrotic lesions are present in diaphragm of mice under 1 month of age. Mice older than 6 months of age display cardiomyopathy. Mice display irregularities of the coronary vasculature.

Heterozygous Phenotype: Heterozygotes do not show any known morphological abnormalities.

Strain of Origin: 129 x C57BL/6 hybrid

Strain genetic background: C57BL/6J

Suggested Control Mice: Wild-type littermates or C57BL/6 mice

Research Applications

  • Cardiovascular
  • Developmental Biology
  • Models for Human Disease
  • Musculoskeletal

Strain Origin

Donor: Kevin P. Campbell, Ph.D., University of Iowa

Primary Reference:

  • Coral-Vazquez R, Cohn RD, Moore SA, Hill JA, Weiss RM, Davisson RL, Straub V, Barresi R, Bansal D, Hrstka RF, Williamson R, Campbell KP. Disruption of the sarcoglycan-sarcospan complex in vascular smooth muscle: a novel mechanism for cardiomyopathy and muscular dystrophy. Cell. 1999 Aug 20;98(4):465-74. (Medline PMID: 10481911)
  • Cohn RD, Durbeej M, Moore SA, Coral-Vazquez R, Prouty S, Campbell KP. Prevention of cardiomyopathy in mouse models lacking the smooth muscle sarcoglycan-sarcospan complex. J Clin Invest. 2001 Jan;107(2):R1-7. (Medline PMID: 11160141)

Colony and Husbandry Information

Special Considerations

Mice with decreased mobility may benefit from gruel as food/water source.

Health Status Report



Mice derived from requested cell lines via Microinjection services will be pathogen tested prior to delivery of mice and health reports will be provided. If you require additional health status information prior to requesting microinjection please email mmrrc@missouri.edu.

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Sib-mating

Breeding Scheme(s):

    Generation: N5

    Overall Breeding Performance: Good

    Reproductive Statistics

    Viability and Fertility:FemaleMale
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes

    Age Reproductive Decline: 6 8

    Average litter size: 6

    Recommended wean age: 3 weeks

    Order Request Information

    Availability Level

    The availability level for this product has not been determined.

    Conditions of Distribution [Including applicable technology transfer agreements]

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    MMRRC Item # - Description Distribution
    Fee/unit (US $)
    Units Notes
    016838-MU-RESUSLitter recovered from cryo-archive
    $2,022.00 / $4,109.00
    Non-Profit / For-Profit
    Litter Recovered litter1; additional fees for any special requests.
    016838-MU-SPERMCryo-preserved spermatozoa
    $437.00 / $817.00
    Non-Profit / For-Profit
    Aliquot Approximate quantity.2

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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    Generated: 04/17/2014