Strain Detail Sheet

Strain Name    :

B6.Cg-Mc1re Adamts20bt-2H/Mmmh

Stock Number :

000184-MU

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Mc1re
Name: recessive yellow
Alteration at locus: Spontaneous Mutation
Allele Symbol: Adamts20bt-2H
Name: belted 2 Harwell
Alteration at locus: Spontaneous Mutation
Gene Symbol: Mc1r
Name: melanocortin 1 receptor
Chromosome: 8
Alteration at locus: Spontaneous Mutation
Gene Symbol: Adamts20
Name: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Chromosome: 15
Alteration at locus: Spontaneous Mutation

Genetic Alterations:
Spontaneous mutations. The Mc1re allele mutation in MMRRC:000184 is a frameshift due to a deletion of a single nucleotide at position 549. This mutation is predicted to result in a protein that continues out of frame for 12 amino acids before terminating. The Adamts20bt-2H allele has a single C to T point mutation at nucleotide 2451, which is predicted to cause a missense mutation (Leu761Phe) in the spacer domain of ADAMTS20.

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype
Recessive yellow (Mc1re) is recessive to nonyellow. In the case of C57BL/6J, nonyellow mice are black. Recessive yellow mice are pheomelanic because of a constitutive inability of the melanocortin 1 receptor, located on the cell surface of the melanocyte and of some tissues in the brain, to respond to signals.

Belted is a recessive mutation that results in white-spotting. White-spotting is defined as the absence of pigmentation that is caused by absence of melanocytes, which is distinct from albinism in which the absence of pigment results from failure of melanogenesis within the pigment cells. The belted locus encodes "a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 20", that is evidently involved in survival of melanocytes. Homozygous mutants usually have a white belly patch and often a white belt across the back in the midtrunk region. The two may coalesce to form a white belt. Two phenotypic characteristics distinguish belted mice from mice mutant at other white-spotting loci. First, the background genome does not seem to influence the location of the spot. Second pheomelanogenesis of the mouse has only a small influence upon the size of the spot.

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: C57BL/6J-Mc1re/Mc1re was obtained from The Jackson Laboratory and was used to create parallel belted stocks MMRRC:000183 and MMRRC:000184. Belted was obtained in 1973 from MRC Radiobiology Unit on a stock that also carried Cattanach's translocation.

Suggested Control Mice:

  • Belted without recessive yellow, MMRRC:000183 .
  • C57BL/6J
  • Yellow not belted (C57BL/6J-Mc1re/J, Stock Number:000060) available at The Jackson Laboratory
  • Backcross to C57BL/6J followed by selection for the desired elements of the four possible recombinants.

Research Applications

  • Pigmentation
  • Neural Crest Development
  • Developmental Biology
  • Eumelanin/pheomelanin differences are the result if cell/cell interactions

Strain Origin

Donor: Dr. Lynn Lamoreux, Texas A&M University

Primary Reference:

  • Lamoreux ML, Russell ES. Developmental interaction in the pigmentary system of mice. I. Interactions between effects of genes on color of pigment and on distribution of pigmentation in the coat of the house mouse (Mus musculus). J Hered. 1979 Jan-Feb;70(1):31-6. (Medline PMID: 469221)
  • Lamoreux ML. Strain-specific white-spotting patterns in laboratory mice. Pigment Cell Res. 1999 Dec;12(6):383-90. (Medline PMID: 10614578)
  • Murray JM, Snell GD. Belted, a new sixth chromosome mutation in the mouse. J Hered 1945;36:266-8 (Medline: Not Available)
  • Rao C, Foernzler D, Loftus SK, Liu S, McPherson JD, Jungers KA, Apte SS, Pavan WJ, Beier DR. A defect in a novel ADAMTS family member is the cause of the belted white-spotting mutation. Development. 2003 Oct;130(19):4665-72. (Medline PMID: 12925592)
  • Silver DL, Hou L, Somerville R, Young ME, Apte SS, Pavan WJ. The secreted metalloprotease ADAMTS20 is required for melanoblast survival. PLoS Genet. 2008 Feb 29;4(2):e1000003. (Medline PMID: [pmid])

Colony and Husbandry Information

Special Considerations

Feed 9% fat breeder chow.

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat color: Yellow with white spot to belt across back and midtrunk

Other:


Breeding

MMRRC Breeding System: Sib mating

Breeding Scheme(s):

  • Homozygous female x Homozygous male

Generation: N>10F>10

Overall Breeding Performance: Poor

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
 
Age Reproductive Decline: 8 months 8 months

Average litter size: 7

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
000184-MU-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
000184-MU-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2
000184-MU-EMBRYOCryo-preserved embryos
$1,038.00 / $2,621.00
Non-Profit / For-Profit
Aliquot Approximate quantity3: 20-40 embryos / aliquot

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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