Strain Name:
C.129S4(B6)-Prkdctm1Get /Mmnc
Stock Number:
030046-UNC
Citation ID:
RRID:MMRRC_030046-UNC

Strain Information

Prkdctm1Get
Name: protein kinase, DNA activated, catalytic polypeptide; targeted mutation 1, Guillermo E Taccioli
Synonyms: DNA-PKcs-
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 16
Alteration at locus: Knockout
Prkdc
Name: protein kinase, DNA activated, catalytic polypeptide
Synonyms: DNA-PKcs, slip, DNAPDcs, XRCC7, DNA-PK, DOXNPH, dxnph
Type: Gene
Species: Mouse
Chromosome: 16
Alteration at locus: Knockout
NCBI: 19090
HGNC: HGNC:9413
Homologene: 5037
Genetic Alterations
A neo construct was inserted into the C-terminal portion of the DNA-PKcs gene and deleted 2 exons that included the Ser/Thr kinase domain and 3'untranslated region. (see Taccioli, G.E. et. al., Immunity Vol.9:355-366, 1998).
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line
    J1 derived from 129S4/SvJae
    Phenotype
    Homozygous: DNA-PKcs-/- mice have a block in T and B cell differentiation that results in a severe combined immunodeficiency phenotype. The DNA-PKcs-/- mouse is phenotypically the same as the classical C.B17 scid mouse.

    Heterozygous: Mice heterozygous/hemizygous for DNA-PKcs-/- exhibit a wild type phenotype in T and B lymphocyte development.
    Mammalian Phenotype Terms
    Allelic Composition: Prkdctm1Get/Prkdctm1Get (Genetic Background: involves: 129S4/SvJae * Black Swiss )

    MeSH Terms
    • Animals
    • B-Lymphocytes
    • DNA Repair
    • DNA-Activated Protein Kinase
    • DNA-Binding Proteins
    • Genes, Immunoglobulin
    • Immune Tolerance
    • Immunoglobulin A/biosynthesis
    • Immunoglobulin Class Switching
    • Immunoglobulin G/biosynthesis
    • Immunoglobulin G/blood
    • Immunoglobulin G/classification
    • Interleukin-4/pharmacology
    • Lipopolysaccharides/pharmacology
    • Mice
    • Mice, Inbred BALB C
    • Mice, SCID
    • Protein-Serine-Threonine Kinases/physiology
    • Recombination, Genetic
    • T-Lymphocytes/physiology
    • Transforming Growth Factor beta/pharmacology
    • Antibodies, Antinuclear/biosynthesis
    • Antibodies, Antinuclear/genetics
    • Antibody Specificity
    • Autoantigens
    • B-Lymphocytes/cytology
    • B-Lymphocytes/immunology
    • Cell Differentiation
    • DNA/immunology
    • Immunoglobulin G/genetics
    • Lymphocyte Activation
    • Mice, Transgenic
    • Severe Combined Immunodeficiency/genetics
    • Severe Combined Immunodeficiency/immunology
    • T-Lymphocytes/immunology
    • Adoptive Transfer
    • Bone Marrow Cells/cytology
    • Breeding
    • Catalytic Domain
    • Cell Count
    • DNA-Activated Protein Kinase/deficiency
    • DNA-Activated Protein Kinase/metabolism
    • DNA-Binding Proteins/deficiency
    • DNA-Binding Proteins/metabolism
    • Flow Cytometry
    • Genotype
    • Immunoglobulin Class Switching/genetics
    • Immunoglobulin Class Switching/immunology
    • Immunoglobulins/blood
    • Nuclear Proteins/deficiency
    • Nuclear Proteins/metabolism
    • Spleen/cytology
    • T-Lymphocytes/cytology
    • Thymus Gland/cytology
    • Transgenes
    • Antibodies, Antinuclear/immunology
    • Antibody Affinity
    • Apoptosis/genetics
    • Apoptosis/immunology
    • Autoimmunity/genetics
    • B-Lymphocytes/transplantation
    • Bone Marrow/immunology
    • Bone Marrow Cells/immunology
    • Gene Rearrangement, B-Lymphocyte, Light Chain/genetics
    • Gene Rearrangement, B-Lymphocyte, Light Chain/immunology
    • Immunoglobulin M/genetics
    • Immunoglobulin M/immunology
    • Lymphocyte Transfusion
    • Mice, Knockout
    • Organ Specificity/genetics
    • Organ Specificity/immunology
    • Receptors, Antigen, B-Cell/genetics
    • Receptors, Antigen, B-Cell/immunology
    • Spleen/immunology
    Strain Development
    The DNA-PKcs-/- founder mouse was constructed in the Taccioli lab (see Taccioli, G. E. et. al., Immunity Vol. 9:355-366, 1998) and backcrossed 6 generations to BALB/c. One additional backcross generation was made in the Bosma lab before intercrossing backcross generation 7 for selection of homozygous DNA-PKcs- /- mice.
    Suggested Control Mice

    Wildtype littermates

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@med.unc.edu. Older strains may not have this information.
    • Apoptosis
    • Cell Biology
    • Hematology
    • Immunology and Inflammation
    • Models for Human Disease
    • Research Tools
    Donor
    Melvin Bosma, Sr., Ph.D., Fox Chase Cancer Center
    Primary Reference
    • Bosma GC, Kim J, Urich T, Fath DM, Cotticelli MG, Ruetsch NR, Radic MZ, Bosma MJ. DNA-dependent protein kinase activity is not required for immunoglobulin class switching. J Exp Med. 2002 Dec 2;196(11):1483-95. (Medline PMID: 12461083)
    • Bosma GC, Oshinsky J, Kiefer K, Nakajima PB, Charan D, Congelton C, Radic M, Bosma MJ. Development of functional B cells in a line of SCID mice with transgenes coding for anti-double-stranded DNA antibody. J Immunol. 2006 Jan 15;176(2):889-98. (Medline PMID: 16393973)
    • Kiefer K, Oshinsky J, Kim J, Nakajima PB, Bosma GC, Bosma MJ. The catalytic subunit of DNA-protein kinase (DNA-PKcs) is not required for Ig class-switch recombination. Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2843-8. Epub 2007 Feb 12. (Medline PMID: 17296939)
    • Kiefer K, Nakajima PB, Oshinsky J, Seeholzer SH, Radic M, Bosma GC, Bosma MJ. Abstract Antigen receptor editing in anti-DNA transitional B cells deficient for surface IgM. J Immunol. 2008 May 1;180(9):6094-106. (Medline PMID: 18424731)

    Colony and Husbandry Information

    SPF environment with autoclaved bedding, water and food.

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*
    Units Notes
    030046-UNC-RESUS Litter recovered from cryo-archive $2,914.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.