Strain Detail Sheet

Strain Name    :

B6.Cg-Axin1Fu-Tg1/CosMmjax

Stock Number :

032035-JAX

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Allele Symbol: Axin1Fu-Tg1
Name: fused transgenic 1
Alteration at locus: Transgenic (random, gene disruption)
Gene Symbol: Axin1
Name: axin 1
Alteration at locus: Transgenic (random, gene disruption)

Genetic Alterations:

Genotype Determination:

ES Cell Line: Not Applicable

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

Homozygous phenotype: The AxinTg1 transgenic insertional allele (previously called FuTg1 or H ε46) has a tandem array of the human epsilon-globin gene (~600 kb in total) inserted between exon 1 and exon 3 (deleting exon 2) of the Axin1 locus. The transgene directs expression of a truncated Axin1 mRNA product (still containing exons 3-10) that results in a recessive loss-of-function allele. Homozygous AxinTg1 mice die between embryonic day (E)8-E10 with abnormalities in developmental patterning (duplication of embryonic axis and parts of the embryo) and nervous system development (truncated headfolds, severely underdeveloped/absent forebrain, and kinked neural tube). Some incidence of cardia bifida and enlarged pericardium are reported in severely affected homozygotes. These AxinTg1 mutant mice may be useful for studying developmental patterning, axis duplication, forebrain development, negative regulation of canonical Wnt signaling pathway, β-catenin/cadherin function, and apoptosis.

Heterozygous phenotype:


Mammalian Phenotype Terms:(provided by MGI)      Extend all MPTs
      assigned by genotype

The following phenotype information may relate to one or more alleles on a genetic background differing from this MMRRC strain.
Axin1Fu-Tg1/Axin1Fu-Tg1
        involves: C57BL/6J * CBA/J
  • mortality/aging
    • complete embryonic lethality during organogenesis (MGI Ref ID J:28303)
      • lethality around E9 to E10
  • growth/size phenotype
    • decreased embryo size (MGI Ref ID J:147293)
    • embryonic growth retardation (MGI Ref IDs J:28303, J:147293)
      • most mutants at E8.5 appear retarded in development, resembling E8 embryos (MGI Ref ID J:28303)
      • developmental delay at E9.5 (MGI Ref ID J:147293)
  • embryogenesis phenotype
    • abnormal allantois morphology (MGI Ref ID J:28303)
      • some exhibit two allantoises connected to the placenta
    • abnormal amniotic cavity morphology (MGI Ref ID J:28303)
      • abnormal accumulation of cellular debris in the amniotic cavity
    • abnormal developmental patterning (MGI Ref ID J:28303)
      • exhibit duplication of the embryonic axis and parts of the embryo
      • abnormal axial mesoderm (MGI Ref ID J:41619)
        • E8.5 embryos exhibit axial duplications
    • abnormal embryonic tissue morphology (MGI Ref ID J:28303)
      • the posterior end of the primary embryo protrudes through the visceral yolk sac in to the yolk cavity
      • some embryos have extra structures near the head folds, often protruding through the yolk sac and in other cases, the head folds appear to protrude through the yolk sac
      • abnormal axial mesoderm (MGI Ref ID J:41619)
        • E8.5 embryos exhibit axial duplications
      • incomplete cephalic closure (MGI Ref ID J:147293)
        • open head folds
      • incomplete cephalic closure (MGI Ref ID J:28303)
        • by E9.5, the head folds usually failed to close
      • kinked neural tube (MGI Ref ID J:28303)
    • decreased embryo size (MGI Ref ID J:147293)
    • embryonic growth retardation (MGI Ref IDs J:28303, J:147293)
      • most mutants at E8.5 appear retarded in development, resembling E8 embryos (MGI Ref ID J:28303)
      • developmental delay at E9.5 (MGI Ref ID J:147293)
  • cardiovascular system phenotype
    • cardia bifida (MGI Ref IDs J:28303, J:147293)
      • (MGI Ref ID J:147293)
      • seen in severely affected mutants (MGI Ref ID J:28303)
    • enlarged pericardium (MGI Ref ID J:147293)
    • enlarged pericardium (MGI Ref ID J:28303)
      • heart appears normal in most cases, however occasionally the pericardium is abnormally expanded
  • nervous system phenotype
    • abnormal forebrain development (MGI Ref ID J:28303)
      • forebrain is absent or severely underdeveloped by E9.5
    • incomplete cephalic closure (MGI Ref ID J:28303)
      • by E9.5, the head folds usually failed to close
    • incomplete cephalic closure (MGI Ref ID J:147293)
      • open head folds
    • kinked neural tube (MGI Ref ID J:28303)
  • craniofacial phenotype
    • shortened head (MGI Ref ID J:147293)
      • truncated head at E9.5

Strain Development: An 8.1 kb transgene harboring a tandem array of the human epsilon-globin gene (~600 kb in total) was microinjected into (CBA/J x C57BL/6J)F2 zygotes, and founder mice were bred to (CBA/J x C57BL/6J)F1 mice. Transgenic founder line 46 (Hε46) was established on a CBA/J;C57BL/6J hybrid background and found to have the transgene inserted between exon 1 and exon 3 of the Axin1 locus; also resulting in deletion of exon 2 and parts of the two flanking introns. Mice heterozygous for this AxinTg1 transgenic insertional allele (previously called FuTg1 or Hε46), were backcrossed to C57BL/6J inbred mice for approximately 20 generations prior cryopreserving heterozygous embryos. Aliquots of these frozen embryos were sent to The Jackson Laboratory Repository and used to establish a live mutant colony.

Suggested Control Mice: Wild-type littermates

Research Applications

    Strain Origin

    Donor: Frank Costantini, Ph.D., Columbia University Medical Center

    Primary Reference:

    Zeng L; Fagotto F; Zhang T; Hsu W; Vasicek TJ; Perry WL 3rd ; Lee JJ ; Tilghman SM ; Gumbiner BM ; Costantini F, The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation., Cell 1997 Jul 11;90(1):181-92 (Medline PMID: 9230313)

    Colony and Husbandry Information

    Special Considerations

    When maintaining a live colony, hemizygous mice may be bred to wildtype siblings or to C57BL/6J inbred mice (Jax Stock No. 000664). Homozygous mice die in utero.

    Health Status Report

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Conditions of Distribution [Including applicable technology transfer agreements]

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # - Description Distribution
    Fee/unit (US $)
    Units Notes
    032035-JAX-RESUSLitter recovered from cryo-archive
    $2,022.00
    Non-Profit
    Litter Recovered litter1; additional fees for any special requests.
    032035-JAX-SPERMCryo-preserved spermatozoa
    $437.00
    Non-Profit
    Aliquot Approximate quantity.2

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

    This site requires JavaScript. Tested for Firefox 3.5+, Microsoft Internet Explorer 7+, Google Chrome.

    Generated: 04/23/2014