Strain Name:
B6.Cg-Axin1Fu-Tg1/CosMmjax
Stock Number:
032035-JAX
Citation ID:
RRID:MMRRC_032035-JAX

Strain Information

Axin1Fu-Tg1
Name: fused transgenic 1
Synonyms: AxinTg1, H epsilon 46
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Transgenic (random, gene disruption)
Axin1
Name: axin 1
Type: Gene
Species: Mouse
Chromosome: 17
Alteration at locus: Transgenic (random, gene disruption)
NCBI: 12005
HGNC: HGNC:903
Homologene: 2614
Genotype Determination
Phenotype
Homozygous: The AxinTg1 transgenic insertional allele (previously called FuTg1 or H ε46) has a tandem array of the human epsilon-globin gene (~600 kb in total) inserted between exon 1 and exon 3 (deleting exon 2) of the Axin1 locus. The transgene directs expression of a truncated Axin1 mRNA product (still containing exons 3-10) that results in a recessive loss-of-function allele. Homozygous AxinTg1 mice die between embryonic day (E)8-E10 with abnormalities in developmental patterning (duplication of embryonic axis and parts of the embryo) and nervous system development (truncated headfolds, severely underdeveloped/absent forebrain, and kinked neural tube). Some incidence of cardia bifida and enlarged pericardium are reported in severely affected homozygotes. These AxinTg1 mutant mice may be useful for studying developmental patterning, axis duplication, forebrain development, negative regulation of canonical Wnt signaling pathway, β-catenin/cadherin function, and apoptosis.

Heterozygous: Undetermined.
Mammalian Phenotype Terms
Allelic Composition: Axin1Fu-Tg1/Axin1Fu-Tg1 (Genetic Background: involves: C57BL/6J * CBA/J )

Allelic Composition: Axin1Fu-Tg1/Axin1tm1(Axin2)Cos (Genetic Background: involves: 129S6/SvEvTac * C57BL/6J * CBA/J * FVB/N )

Allelic Composition: Axin1Fu-ki/Axin1Fu-Tg1 (Genetic Background: involves: BTBR * C57BL/6J * CBA/J )

MeSH Terms
  • Amino Acid Sequence
  • Amphibians
  • Animals
  • Axin Protein
  • Chickens
  • Cloning, Molecular
  • Embryo, Nonmammalian/physiology
  • Embryonic Induction
  • Humans
  • Mammals
  • Mice
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Protein Biosynthesis
  • Proteins/chemistry
  • Proteins/genetics
  • RNA, Messenger/biosynthesis
  • Rats
  • Recombinant Proteins/biosynthesis
  • Recombinant Proteins/chemistry
  • Repressor Proteins
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Signal Transduction
  • Stem Cells/physiology
  • Xenopus
  • Xenopus Proteins
Strain Development
An 8.1 kb transgene harboring a tandem array of the human epsilon-globin gene (~600 kb in total) was microinjected into (CBA/J x C57BL/6J)F2 zygotes, and founder mice were bred to (CBA/J x C57BL/6J)F1 mice. Transgenic founder line 46 (Hε46) was established on a CBA/J;C57BL/6J hybrid background and found to have the transgene inserted between exon 1 and exon 3 of the Axin1 locus; also resulting in deletion of exon 2 and parts of the two flanking introns. Mice heterozygous for this AxinTg1 transgenic insertional allele (previously called FuTg1 or Hε46), were backcrossed to C57BL/6J inbred mice for approximately 20 generations prior cryopreserving heterozygous embryos. Aliquots of these frozen embryos were sent to The Jackson Laboratory Repository and used to establish a live mutant colony.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact csmmrrc@jax.org. Older strains may not have this information.
Donor
Frank Costantini, Ph.D., Columbia University Medical Center
Primary Reference
Zeng L; Fagotto F; Zhang T; Hsu W; Vasicek TJ; Perry WL 3rd ; Lee JJ ; Tilghman SM ; Gumbiner BM ; Costantini F, The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation., Cell 1997 Jul 11;90(1):181-92 (Medline PMID: 9230313)

Colony and Husbandry Information

When maintaining a live colony, hemizygous mice may be bred to wildtype siblings or to C57BL/6J inbred mice (Jax Stock No. 000664). Homozygous mice die in utero.

Cryo-recovered strains distributed by the MMRRC at JAX are shipped to the customer from the Pathogen & Opportunistic-Free Animal Room G200 - see https://www.jax.org/jax-mice-and-services/customer-support/customer-service/animal-health/health-status-reports.

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
032035-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032035-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.