Strain Name:
STOCK Itpr3tm1.1Rmnc/Mmjax
Stock Number:
032884-JAX
Citation ID:
RRID:MMRRC_032884-JAX

Gene Information

Itpr3tm1.1Rmnc
Name: inositol 1,4,5-triphosphate receptor 3; targeted mutation 1.1, Rocky Mountain Regional Neurological Disorders Center
Synonyms: IP3R3tm1(tauGFP), Itpr3tm1.1(Mapt/GFP)Rmnc
Type: Allele
Species: Mus musculus (mouse)
Alteration at locus: Knock-In
Itpr3
Name: inositol 1,4,5-triphosphate receptor 3
Synonyms: Itpr-3, Ip3r3, tf
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
Alteration at locus: Knock-In
NCBI: 16440
HGNC: HGNC:6182
Homologene: 1675
ES Cell Line
EC7.1 Derived From (C57BL/6J x 129X1/SvJ)F1
Phenotype
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Expression of the microtubule-associated protein tau/Green Fluorescent Protein fusion gene, "tauGFP" is controlled by Itpr3 regulatory elements. GPF expression is directed to microvillous cells in the olfactory epithelium. This mutant mouse strain may be useful in studies of taste transduction.

Heterozygote: Heterozygote phenotype is expected to be similar to homozygote phenotype.

Mammalian Phenotype Terms
Allelic Composition: Itpr3tm1.1Rmnc/Itpr3+ (Genetic Background: involves: 129X1/SvJ * C57BL/6 * C57BL/6J )

Strain Development
A targeting vector containing a tau (Mapt)/GFP fusion protein followed by three polyA sites and a loxP-flanked PGKneo cassette was used to replace exon 1. The construct was electroporated into (C57BL/6 x 129X1/SvJ)F1 derived EC7.1 embryonic stem (ES) cells. The resulting chimeric animals were crossed to FVB/N-Tg(ACTB-cre)2Mrt/J mice to remove the neomycin cassette. The strain then was crossed to C57BL/6 mice for two generations. The cre transgene is segregating in this strain. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Suggested Control Mice
Wild-type littermates
  • Cell Biology
  • Developmental Biology
  • Neurobiology
  • Chemical Senses
Donor
Diego Restrepo, Ph.D., University of Colorado, Denver

Colony and Husbandry Information

While maintaining a live colony, these mice are bred as homozygotes.
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Bred to Homozygosity
No

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
032884-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
032884-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.