Strain Name    : B6.129X1-Trp53tm1Ldo/Mmnc

Stock Number : 000362-UNC

Gene Information

(provided by MGI)
Allele Symbol: Trp53tm1Ldo
Name: targeted mutation 1, Lawrence A Donehower
Chromosome: 11
Alteration at locus: Knockout
Gene Symbol: Trp53
Name: transformation related protein 53
Chromosome: 11
Alteration at locus: Knockout
Genetic Alterations:
Exons 1-6 of the p53 gene are deleted as well as about 500 kb of upstream sequence. The upstream Vamp2 promoter is juxtaposed next to exons 7-11 of p53 to generate a chimeric message that produces a C-terminal truncated p53 protein.

Genotype Determination:

  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
  • ES Cell Line: AB1

    Phenotype:

    p53+/m are normal up to the age of about 12 months. From 14-18 months of age, the mutant mice begin to exhibit early aging phenotypes and resistance to spontaneous cancers. Early aging phenotypes include reduced longevity, osteoporosis, atrophy of skeletal muscle, skin, and many organs, and reduced tolerance for stresses.

    Mammalian Phenotype Terms:
    Allelic Composition: Trp53tm1Ldo/Trp53+ (Genetic Background: B6.129S7-Trp53tm1Ldo )

    Allelic Composition: Trp53tm1Ldo/Trp53+ (Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 )

    Allelic Composition: Trp53tm1Brd/Trp53tm1Ldo (Genetic Background: involves: 129S7/SvEvBrd * C57BL/6 )

    Strain of Origin: 129X1/SvJ

    Strain genetic background: C57BL/6J

    Suggested Control Mice:

    • Wildtype littermates

    • Cancer
    • Accelerated or premature aging

    Donor: Larry Donehower, Ph.D., Baylor College of Medicine

    Primary Reference:

    Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann H, Lu X, Soron G, Cooper B, Brayton C, Hee Park S, Thompson T, Karsenty G, Bradley A, Donehower LA. p53 mutant mice that display early ageing-associated phenotypes. Nature. 2002 Jan 3;415(6867):45-53. (Medline PMID: 11780111 )

    Colony and Husbandry Information

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Coat color: Black

    Other:


    MMRRC Breeding System: Intra strain mating

    Breeding Scheme(s):

    • Heterozygous female x Wildtype male
    • Wildtype female x Heterozygous male

    Generation: N5

    Overall Breeding Performance: Normal

    Viability and Fertility:FemaleMaleComments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
     
    Age Reproductive Decline: 16 weeks 16 weeks

    Average litter size: 6 -8

    Recommended wean age: 3 weeks

    Order Request Information

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description
    Distribution
    Fee/unit (US $)
    Units Notes
    000362-UNC-RESUSLitter recovered from cryo-archive
    $2,022.00 / $4,109.00
    Non-Profit / For-Profit
    Litter Recovered litter1; additional fees for any special requests.

    1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.