Allele Symbol: Trp53tm1Ldo
Name: targeted mutation 1, Lawrence A Donehower
Alteration at locus: Knockout
Gene Symbol: Trp53
Name: transformation related protein 53
Alteration at locus: Knockout
ES Cell Line: AB1
p53+/m are normal up to the age of about 12 months. From 14-18 months of age, the mutant mice begin to exhibit early aging phenotypes and resistance to spontaneous cancers. Early aging phenotypes include reduced longevity, osteoporosis, atrophy of skeletal muscle, skin, and many organs, and reduced tolerance for stresses.
Strain of Origin: 129X1/SvJ
Strain genetic background: C57BL/6J
Suggested Control Mice:
Donor: Larry Donehower, Ph.D., Baylor College of Medicine
Tyner SD, Venkatachalam S, Choi J, Jones S, Ghebranious N, Igelmann H, Lu X, Soron G, Cooper B, Brayton C, Hee Park S, Thompson T, Karsenty G, Bradley A, Donehower LA. p53 mutant mice that display early ageing-associated phenotypes. Nature. 2002 Jan 3;415(6867):45-53. (Medline PMID: 11780111 )
Coat color: Black
MMRRC Breeding System: Intra strain mating
Overall Breeding Performance: Normal
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes||Yes|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||16 weeks||16 weeks|
Average litter size: 6 -8
Recommended wean age: 3 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.