Strain Name:
FVB/N-Prkg1Tn(sb-rtTA)2497B.SB2Ove/Mmjax
Stock Number:
036283-JAX
Citation ID:
RRID:MMRRC_036283-JAX

Gene Information

Prkg1Tn(sb-rtTA)2497B.SB2Ove
Name: protein kinase, cGMP-dependent, type I; transposon insertion 2479B.SB2, Paul A Overbeek
Synonyms: OVE2497B-SB2, OVE#2497B-SB2
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Insertion
Prkg1
Name: protein kinase, cGMP-dependent, type I
Synonyms: Prkgr1b, Prkg1b
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
Alteration at locus: Insertion
NCBI: 19091
HGNC: HGNC:9414
Homologene: 55964
Genetic Alterations
These OVE2497B-SB2 (OVE#2497B-SB2) mice harbor a mutation created by random insertion of the SB-sa-IRES-rtTA-pA-SB-Tyro-WPRE-FUGW lentiposon transgene (LV2223), and the transposon was subsequently mobilized via exposure to sleeping beauty transposase (SB10) to generate the mutation. Using inverse PCR analysis, the transposon integration site was identified in intron 10 of the protein kinase, cGMP-dependent, type I gene (Prkg1) on chromosome 19. The IR/DR is linked to the (-) strand of DNA at position 30,692,871 bp [NCB137/mm9; 30,692,871(-)]. The rtTA is inserted in the sense orientation relative to the disrupted mouse gene.
Phenotype
The donating investigator reports the phenotype of homozygous mice as: post-weaning lethal.
Mammalian Phenotype Terms
Allelic Composition: Prkg1Tn(sb-rtTA)2497B.SB2Ove/Prkg1Tn(sb-rtTA)2497B.SB2Ove (Genetic Background: involves: FVB/N )

  • mortality/aging
Strain Development
A lentiviral transgenic approach was used to generate these mice. The SB-sa-IRES-rtTA-pA-SB-Tyro-WPRE-FUGW lentiposon transgene (LV2223) was designed with a Sleeping Beauty (SB) transposon (containing a slice-acceptor::IRES::rtTA::polyA gene trap), a mouse tyrosinase minigene (Tyro), and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) in the FUGW self-inactivating HIV-based lentiviral vector backbone. The SB transposon and Tyro minigene replaced the ubiquitin-c promoter and EGFP sequences originally found in the FUGW lentiviral vector. The 1200 bp SB transposon used in this transgene has an inverted repeat/direct repeat sequence (IR/DR; the SB transposon recognition site), an adenovirus splice acceptor, a stop sequence (3xSTOP), an internal ribosome entry site (IRES; from human X-chromosome-linked inhibitor of apoptosis (XIAP)), a sequence encoding an optimized form of reverse tetracycline controlled transactivator (rtTA2S; with stop codon), a human growth hormone polyA sequence, and a second IR/DR sequence. The IR/DR sequences are outward-facing (pointed away from the sa-IRES-rtTA-pA). The Tyro minigene is composed of the mouse Tyr enhancer region (623 bp), promoter region (657 bp), and 1566 bp cDNA sequence (including the stop codon); all in sense orientation relative to the FUGW backbone. There is no polyA site between the Tyro minigene and WPRE sequence. The WPRE sequence functions to enhance the mRNA transcript stability. This packaged lentiposon was injected subzonally (under the zona pellucida) into 1-8 cell stage FVB/N mouse embryos. Expression of the tyrosinase minigene results in melanin synthesis, so founder mice (F0) were identified by inspection for pigmentation. All F0 mice exhibited mosaic, light, pigmentation. Most F0 mice had more than one lentiviral integration site. F0 mice were assigned an OVE number and then bred with FVB/N mice. Pigmented offspring (F1) with different coat colors were designated as subline A, B, C, etc., for each family. F1 mice were bred with FVB/N mice. F2 mice with different coat colors were designated as sublines A-1, A-2, etc., or B-1, B-2, etc. Breeding with FVB/N mice was continued until litters with only one or two different coat colors were obtained. Mice with identical coat colors were then inbred to generate homozygotes. Homozygous mice were viable and fertile. Homozygous females were bred to males with testes-specific expression of the SB transposase (PGK2-SB10 transgenic line OVE1780 on an FVB/N genetic background). The resulting double mutant male offspring ("seed males") have the ability to mobilize transposons in their germline; and mobilization of the outward-facing, IR/DR site-flanked transposon results in integration at new sites in the genome. The seed males were bred with FVB/N wildtype females, and the offspring were screened for presence of the original lentiviral insertion site (positive for tyrosinase) and for transposition of the splice-acceptor::IRES::rtTA::polyA transposon. Mice with transpositions (new F0 mice) were bred to FVB/N mice to establish new lines (SB1, SB2, etc.). If the new integration site is still linked to the original lentiviral integration site, the mice are pigmented. If the original lentiviral integration has been bred away from the new integration site, the mice are albino. F1 mice for each new line were inbred and assessed for the presence or absence of viable homozygotes. Hemizygous mice of line OVE2497B-SB2 (OVE#2497B-SB2) exhibit medium grey coat color. The donating investigator reports the PGK2-SB10 transgene was bred out of the line as well. Using inverse PCR analysis, the transposon integration site was identified in intron 10 of the protein kinase, cGMP-dependent, type I gene (Prkg1) on chromosome 19. The IR/DR is linked to the (-) strand of DNA at position 30,692,871 bp [NCB137/mm9; 30,692,871(-)]. The rtTA is inserted in the sense orientation relative to the disrupted mouse gene. Transgenic males were sent to The Jackson Laboratory Repository node of the Mutant Mouse Regional Resource Center (MMRRC-JAX). Upon arrival, these mice were bred to FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the live colony.
Suggested Control Mice
Wild-type littermates or FVB/NJ
  • protein kinase, cGMP regd
Donor
Paul A. Overbeek, Ph.D., Baylor College of Medicine

Colony and Husbandry Information

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Medium Grey
MMRRC Breeding System
When maintaining a live colony, heterozygous mice may be bred together, bred with wildtype siblings, or bred with FVB/N inbred mice (Stock No. 001800).
Breeding Scheme(s)
  • Heterozygous female x Heterozygouns male
  • Heterozygous female x male wild-type from colony male or recipricol mating
  • Heterozygous female x FVB/NJ male or recipricol mating
Overall Breeding Performance
Unknown
Viability and Fertility: Female Male Comments
Homozygotes are viable: Reduced Reduced
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Unknown Unknown
Average litter size
Unknown
Recommended wean age
Unknown

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036283-JAX-SPERM Cryo-preserved spermatozoa $437.00 / Non-Profit Aliquot Approximate quantity3
036283-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to csmmrrc@jax.org for more information.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.