Strain Name:
Stock Number:
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Strain Information

Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 9, Mark A Magnuson
Type: Allele
Species: Mus musculus (mouse)
Alteration at locus: Targeted Mutation
Name: neurogenin 3
Synonyms: ngn3, Atoh5, bHLHa7, Math4B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
Alteration at locus: Targeted Mutation
NCBI: 11925
VEGA: 10
Homologene: 40692
Name: tetracycline operon
Type: Gene
Species: Synthetic
Alteration at locus: Targeted Mutation
Genetic Alterations
This mouse contains a bidrectional TetO-regulated fusion gene inserted into a disabled Rosa26 locus. In one direction the tetO/CMV promoter drives expression of CFP(cerulean). In the other direction it drives expression of Ngn3.
Genotype Determination
ES Cell Line
TL1/TL-1 derived from 129S6/SvEvTac

Homozygous: This mouse is homozygous lethal.

Heterozygous: Normal

MeSH Terms
  • Alleles
  • Animals
  • Chromosomes, Artificial, Bacterial/genetics
  • Embryo, Mammalian/metabolism
  • Embryonic Stem Cells/cytology
  • Embryonic Stem Cells/metabolism
  • Fluorescence
  • Genetic Loci/genetics
  • Genetic Vectors/genetics
  • Luminescent Proteins/metabolism
  • Mice
  • Mutagenesis, Insertional/methods
  • Organ Specificity
  • Proteins/genetics
  • RNA, Untranslated
  • Rabbits
  • Recombinases/metabolism
Strain Development
This strain is a mixed genetic background of both 129S6 and C57Bl/6J. Four backcrosses have been performed with plans for more matings before line submission.
Suggested Control Mice
Wild-type littermates
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact Older strains may not have this information.
  • Diabetes/Obesity
  • Internal Organ
  • Research Tools
Mark Magnuson, M.D., Vanderbilt University.
Primary Reference

Chen SX, Osipovich AB, Potter LA, Hipkens S, Gangula R, Yuan W, Piston DW, Magnuson MA. Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. Dis Model Mech 2011 Jul; 4(4);537-47. Epub 2011 Feb 14. (Mediline PMID: 21324933).

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email .
Coat Color
Black or Agouti
MMRRC Breeding System
Breeding Scheme(s)

C57BL/6J female x Heterozygous male

Overall Breeding Performance
Viability and Fertility: Female Male Comments
Homozygotes are viable: No No
Homozygotes are fertile: No No
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 8 months 8 months
Average litter size
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

TET-System Technology (tetracycline; US 5,814,618; 5,859,310; 7,541,446; 8,383,364 and European Patent EP 0804565)

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
036636-MU-RESUS Litter recovered from cryo-archive $2,624.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to for more information.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.