Strain Detail Sheet

Strain Name    :

C57BL/6ByJ-Chr 7129P3/J/MonMmjax

Stock Number :

036689-JAX

Other Names   :

C57BL6/ByJ-Chr 7

Gene Information

Gene Details [Including genotyping protocols]

(provided by MGI)
Chromosome Symbol: Ch 7129P3/J/Mon
Name:
Chromosome: 7
Alteration at locus: Chromosome Substitution
QTL Symbol: Ap7q
Name: alcohol preference 7 QTL
Chromosome: 7
Alteration at locus: Chromosome Substitution
QTL Symbol: Adip3
Name: adiposity 3
Chromosome: 7
Alteration at locus: Chromosome Substitution
QTL Variant Symbol: Ap7q129P3/J
Name: alcohol preference 7 QTL; 129P3/J
Chromosome: 7
Alteration at locus: Chromosome Substitution
QTL Variant Symbol: Adip3129P3/J
Name: adiposity 3; 129P3/J
Chromosome: 7
Alteration at locus: Chromosome Substitution

Genetic Alterations:
This strain represents one of a set of 7 consomic strains in which progenitor inbred strains C57BL/6ByJ (JAX Stock # 001139) and 129P3/J (JAX Stock # 000690) were used to construct reciprocal chromosome substitution strains (CSSs) for Chr 1, 2, 7 and 9 using a "speed consomics" approach.  In this strain, chromosome 7 was moved from donor strain 129P3/J to host strain C57BL/6ByJ.  View poster (referenced below) for additional information describing this set of CSSs.

Genotype Determination:

ES Cell Line:

Strain Description [Including phenotype, strain background, strain development and suggested control mice]

Phenotype

The 129P3/J and C57BL/6ByJ progenitor inbred strains differ in taste responses, ingestive behavior, alcohol consumption, body size, adiposity, and other traits. Genome scans of crosses between these strains have detected QTLs for these phenotypes that cluster on Chr 1, 2, 7 and 9 (Adip2; Adip3, Adip5, Ap7q, Bwq5, Bwq6, Nattq1, Nattq2, Sucq).

Strain of Origin: C57BL/6ByJ

Strain genetic background: C57BL/6ByJ

Strain Development: Our breeding scheme was designed to eliminate Y chromosome from the donor strain. The 129P3/J and C57BL/6ByJ progenitor inbred strains were crossed and F1 females were backcrossed to males from a host strain, C57BL/6ByJ. Male offspring with a non-recombinant chromosome derived from the donor strain were identified in each backcross generation by genotyping and were backcrossed to females from the host strain, C57BL/6ByJ. To facilitate elimination of the donor, 129P3/J genome in non-target chromosomes using a "speed consomic" approach, we conducted genome scans of the N2 and N3 backcross generations (948 SNPs covering 19 autosomes and the X chromosome were genotyped to identify optimal breeders). Males with a non-recombinant donor chromosome and the smallest proportion of the donor genome in rest of the chromosomes were used as founders of the next generation. In subsequent backcrosses, donor chromosomes (1, 2, 7, 9) were genotyped with sets of SNP markers. We used the most centromeric and the most telomeric polymorphic markers available. To prevent loss of donor chromosome fragments due to double-crossovers, we calculated the cross-specific genetic recombination distances between markers and selected internal markers spaced no more than 10 cM. At the N6 backcross generation, males and females with the non-recombinant donor chromosome were intercrossed. Progeny of this intercross that are homozygous for the donor, 129P3/J chromosome was used to propagate the consomic strains.

Suggested Control Mice: C57BL/6ByJ

Research Applications

  • Diabetes/Obesity
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Research Tools
  • Sensorineural

Strain Origin

Donor: Alexander Bachimanov, Ph.D., D.V.M., Monell Chemical Senses Center

Primary Reference:

  • Lin C, Bosak NP, Nelson TM, Theodorides ML, Smith ZH, Kirkey M, Avigdor M, Gantick B, Khoshnevisan A, Lysenko A, Reed DR, Bachmanov AA. Construction of reciprocal chromosome substitution strains from 129P3/J and C57BL/6ByJ mice. Mouse Genetics Conference. Poster Abstract 236C. Washington D.C., June 22-25, 2011.
  • Reed DR, Avigdor M, McDaniel AH, Bachmanov AA. QTL for body composition on chromosome 7 detected using a chromosome substitution mouse strain. Obesity. 2008 Feb;16(2):483-487. (Medline PMID: 18239664).
  • McDaniel AH, Li X, Tordoff MG, Bachmanov AA, Reed DR. A locus on mouse chromosome 9 (Adip5) affects the relative weight of the gonadal but not retroperitoneal adipose depots. Mammalian Genome. 2006 Nov; 17(11):1078-1092. Epub 2006 Nov 10. (Medline PMID: 17103052).

Colony and Husbandry Information

Special Considerations

Note:  the reciprocal chromosome substitution strains (CSSs) in this set include:

Health Status Report

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org.

Appearance

Coat color: Black

Other:

Breeding

MMRRC Breeding System: Inbred sib-mating

Breeding Scheme(s):

  • Female x Male siblings

Generation: N6

Overall Breeding Performance: Good

Reproductive Statistics

Viability and Fertility:FemaleMale
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes

Age Reproductive Decline: 6 months 12 months

Average litter size: 4-6

Recommended wean age: 3 weeks

Order Request Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Conditions of Distribution [Including applicable technology transfer agreements]

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Fees

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # - Description Distribution
Fee/unit (US $)
Units Notes
036689-JAX-RESUSLitter recovered from cryo-archive
$2,022.00 / $4,109.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.
036689-JAX-SPERMCryo-preserved spermatozoa
$437.00 / $817.00
Non-Profit / For-Profit
Aliquot Approximate quantity.2

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



The MMRRC is a collaborative effort, funded by grants from DPCPSI of the NIH.

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