Strain Name:
C57BL/6ByJ-Chr 7129P3/J/MonMmjax
Stock Number:
036689-JAX
Citation ID:
RRID:MMRRC_036689-JAX
Other Names:
C57BL6/ByJ-Chr 7

Gene Information

Chromosome Symbol: Chr 7129P3/J/Mon (Mus musculus (mouse))
Chromosome: 7
Alteration at locus: Chromosome Substitution
Monarch Initiative (Disease and Phenotype Associations):
QTL Symbol: Ap7q (Mus musculus (mouse))
Name: alcohol preference 7 QTL
Chromosome: 7
Alteration at locus: Chromosome Substitution
Monarch Initiative (Disease and Phenotype Associations): MGI:2155164
QTL Symbol: Adip3 (Mus musculus (mouse))
Name: adiposity 3
Chromosome: 7
Alteration at locus: Chromosome Substitution
Monarch Initiative (Disease and Phenotype Associations): MGI:2149043
QTL Variant Symbol: Ap7q129P3/J (Mus musculus (mouse))
Name: alcohol preference 7 QTL; 129P3/J
Chromosome: 7
Alteration at locus: Chromosome Substitution
Monarch Initiative (Disease and Phenotype Associations): MGI:2668896
QTL Variant Symbol: Adip3129P3/J (Mus musculus (mouse))
Name: adiposity 3; 129P3/J
Chromosome: 7
Alteration at locus: Chromosome Substitution
Monarch Initiative (Disease and Phenotype Associations):
Genetic Alterations

This strain represents one of a set of 7 consomic strains in which progenitor inbred strains C57BL/6ByJ and 129P3/J were used to construct reciprocal chromosome substitution strains (CSSs) for Chr 1, 2, 7 and 9 using a "speed consomics" approach. In this strain, chromosome 7 was moved from donor strain 129P3/J to host strain C57BL/6ByJ.

Genotype Determination
ES Cell Line
N/A
Phenotype
The 129P3/J and C57BL/6ByJ progenitor inbred strains differ in taste responses, ingestive behavior, alcohol consumption, body size, adiposity, and other traits. Genome scans of crosses between these strains have detected QTLs for these phenotypes that cluster on Chr 1, 2, 7 and 9 (Adip2; Adip3, Adip5, Ap7q, Bwq5, Bwq6, Nattq1, Nattq2, Sucq).
MeSH Terms
  • Adiposity/genetics
  • Animals
  • Body Composition/genetics
  • Body Size/genetics
  • Body Weight/genetics
  • Chromosome Mapping/methods
  • Chromosomes, Mammalian/genetics
  • Female
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Quantitative Trait Loci/genetics
  • Adipose Tissue/anatomy & histology
  • Body Constitution
  • Chromosomes, Mammalian
  • Gonads/anatomy & histology
  • Intra-Abdominal Fat/anatomy & histology
  • Lod Score
  • Molecular Sequence Data
  • Obesity/genetics
  • Organ Size/genetics
  • Quantitative Trait Loci
Strain Development
Our breeding scheme was designed to eliminate Y chromosome from the donor strain. The 129P3/J and C57BL/6ByJ progenitor inbred strains were crossed and F1 females were backcrossed to males from a host strain, C57BL/6ByJ. Male offspring with a non-recombinant chromosome derived from the donor strain were identified in each backcross generation by genotyping and were backcrossed to females from the host strain, C57BL/6ByJ. To facilitate elimination of the donor, 129P3/J genome in non-target chromosomes using a "speed consomic" approach, we conducted genome scans of the N2 and N3 backcross generations (948 SNPs covering 19 autosomes and the X chromosome were genotyped to identify optimal breeders). Males with a non-recombinant donor chromosome and the smallest proportion of the donor genome in rest of the chromosomes were used as founders of the next generation. In subsequent backcrosses, donor chromosomes (1, 2, 7, 9) were genotyped with sets of SNP markers. We used the most centromeric and the most telomeric polymorphic markers available. To prevent loss of donor chromosome fragments due to double-crossovers, we calculated the cross-specific genetic recombination distances between markers and selected internal markers spaced no more than 10 cM. At the N6 backcross generation, males and females with the non-recombinant donor chromosome were intercrossed. Progeny of this intercross that are homozygous for the donor, 129P3/J chromosome was used to propagate the consomic strains.
Suggested Control Mice
C57BL/6ByJ
  • Diabetes/Obesity
  • Metabolism
  • Models for Human Disease
  • Neurobiology
  • Research Tools
  • Sensorineural
Donor
Alexander Bachimanov, Ph.D., D.V.M., Monell Chemical Senses Center
Primary Reference
  • Reed DR, Avigdor M, McDaniel AH, Bachmanov AA. QTL for body composition on chromosome 7 detected using a chromosome substitution mouse strain. Obesity (Silver Spring). 2008 Feb;16(2):483-7. (Medline PMID: 18239664).
  • McDaniel AH, Li X, Tordoff MG, Bachmanov AA, Reed DR. A locus on mouse chromosome 9 (Adip5) affects the relative weight of the gonadal but not retroperitoneal adipose depots. Mammalian Genome. 2006 Nov; 17(11):1078-1092. Epub 2006 Nov 10. (Medline PMID: 17103052).
  • Lin C, Bosak NP, Nelson TM, Theodorides ML, Smith ZH, Kirkey M, Avigdor M, Gantick B, Khoshnevisan A, Lysenko A, Reed DR, Bachmanov AA. Construction of reciprocal chromosome substitution strains from 129P3/J and C57BL/6ByJ mice. Poster, Mouse Genetics Conference. Poster Abstract 236C. Washington D.C., June 22-25, 2011.

Colony and Husbandry Informationon

Note: the reciprocal chromosome substitution strains (CSSs) in this set include:
Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email csmmrrc@jax.org .
Coat Color
Black
MMRRC Breeding System
Inbred sib-mating
Breeding Scheme(s)
  • Female x Male siblings
Generation
N6
Overall Breeding Performance
Good
Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: 6 months 12 months
Average litter size
4-6
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
036689-JAX-SPERM Cryo-preserved spermatozoa $437.00 / $437.00
Non-Profit / For-Profit
Aliquot Approximate quantity2
036689-JAX-RESUS Litter recovered from cryo-archive $2,022.00 / $2,022.00
Non-Profit / For-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.