Strain Name    : C57BL/6J-Rorcm1Btlr/Mmmh

Stock Number : 037090-MU

Other Names   : chestnut

Gene Information

(provided by MGI)
Allele Symbol: Rorcm1Btlr
Name: mutation 1, Bruce Beutler
Chromosome: 3
Alteration at locus: Chemically Induced
Gene Symbol: Rorc
Name: RAR-related orphan receptor gamma
Chromosome: 3
Alteration at locus: Chemically Induced
Genetic Alterations:
The chestnut mutation is a T to C transition at base pair 94,377,609 (v38) on chromosome 3 within the linear genomic DNA sequence of Rorc (GenBank genomic region NC_000069). The mutation affects the intron 1 donor splice site of the Rorc splice variant encoding Rorγt (GTAAGT-->GTAAGC; ENSMUST00000107292), but has no effect on the splice variant encoding Rorγ1. The effects of the mutation at the cDNA and protein level have not been tested, but weakening of the intron 1 donor splice site may result in retention of intron 1 in the spliced mRNA. Alternatively, utilization of cryptic splice site(s) may occur. One specific possibility is splicing from a cryptic splice donor site in the 5’ UTR to the normal splice acceptor site in intron 1, resulting in skipping of exon 1 and removal of the normal ATG translation start site in the Rorγt mRNA. An alternative ATG is present in exon 3. Use of this start would frame-shift the protein and result in truncation of the protein 87 amino acids following the methionine start site (within exon 4).

Genotype Determination:

ES Cell Line:

Phenotype

Homozygous: The chestnut phenotype was initially identified among G3 mice carrying mutations induced by N-ethyl-N-nitrosourea (ENU) and tested in the flow cytometric screen for deficiencies of peripheral blood cells. Chestnut mice exhibited deficiency in CD4+ and CD8+ T cells ; other hematopoietic cell subsets were not significantly changed compared to the wild-type controls.

Heterozygous: Not characterized.

 

Mammalian Phenotype Terms:
Allelic Composition: Rorcm1Btlr/Rorcm1Btlr (Genetic Background: C57BL/6J-Rorcm1Btlr )

  • hematopoietic system
    • decreased CD4-positive, alpha beta T cell number [MP:0008075]
    • decreased CD8-positive, alpha-beta T cell number [MP:0008079]
  • immune system
    • decreased CD4-positive, alpha beta T cell number [MP:0008075]
    • decreased CD8-positive, alpha-beta T cell number [MP:0008079]

Strain genetic background: C57BL/6J

Strain Development: Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.

Suggested Control Mice: Wild-type littermates or C57BL/6J mice

  • Developmental Biology
  • Immunology and Inflammation

Donor: Bruce Beutler, M.D., University of Texas Southwestern Medical Center.

Primary Reference:

AZeng M, Wang KW, Murray AR, Beutler B. Record for chestnut. MUTAGENETIX™, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Coat color: Black

Other: None

MMRRC Breeding System: Random intra-strain mating

Breeding Scheme(s):

  • Wild-type female x Heterozygous male from the colony or reciprocal mating
  • Homozygous female x Homozygous male from the colony

Generation:

Overall Breeding Performance: Good

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined

Average litter size: 4-6

Recommended wean age: 3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, see Fees section for further details.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
037090-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.
037090-MU-SPERMCryo-preserved spermatozoa
$437.00
Non-Profit
Aliquot Approximate quantity.2

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section above). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.



To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.