Allele Symbol: Rorcm1Btlr
Name: mutation 1, Bruce Beutler
Alteration at locus: Chemically Induced
Gene Symbol: Rorc
Name: RAR-related orphan receptor gamma
Alteration at locus: Chemically Induced
ES Cell Line: Not applicable
Homozygous: The chestnut phenotype was initially identified among G3 mice carrying mutations induced by N-ethyl-N-nitrosourea (ENU) and tested in the flow cytometric screen for deficiencies of peripheral blood cells. Chestnut mice exhibited deficiency in CD4+ and CD8+ T cells ; other hematopoietic cell subsets were not significantly changed compared to the wild-type controls.
Heterozygous: Not characterized.
Mammalian Phenotype Terms:
Strain of Origin: C57BL/6J
Strain genetic background: C57BL/6J
Strain Development: Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.
Suggested Control Mice: Wild-type littermates or C57BL/6J mice
Coat color: Black
MMRRC Breeding System: Random intra-strain mating
Overall Breeding Performance: Good
|Viability and Fertility:||Female||Male||Comments|
|Homozygotes are viable:||Yes||Yes|
|Homozygotes are fertile:||Yes||Yes|
|Heterozygotes are fertile:||Yes||Yes|
|Age Reproductive Decline:||Undetermined||Undetermined|
Average litter size: 4-6
Recommended wean age: 3-4 weeks
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Some MMRRC strains were submitted by the donor as cryopreserved germplasm, see Fees section for further details.
Fee/unit (US $)
|Litter||Recovered litter1; additional fees for any special requests.|
Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.
1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.