Strain Name    : C57BL/6J-Rag2m1Btlr/Mmmh

Stock Number : 037091-MU

Other Names   : snowcock

Gene Information

(provided by MGI)
Allele Symbol: Rag2m1Btlr
Name: mutation 1, Bruce Beutler
Chromosome: 2
Alteration at locus: Chemically Induced
Gene Symbol: Rag2
Name: recombination activating gene 2
Chromosome: 2
Alteration at locus: Chemically Induced
Genetic Alterations:
The mutation is a T to G transversion at base pair 101,630,603 (v38) on chromsome 2, or base pair 5,856 in the GenBank genomic region NC_000068 encoding Rag2. The mutation corresponds to residue 1,468 in the NM_009020 mRNA sequence (equivalent to residue 1498 in the ENSMUST00000044031 cDNA sequence) in exon 3 of 3 total exons and residue 1407 in the ENSMUST00000111227 cDNA sequence in exon 2 of 2 total exons. The snowcock mutation results in a cysteine (C) to tryptophan (W) substitution at amino acid 419 in both isoforms of Rag2.

Genotype Determination:

ES Cell Line: Not Applicable

Phenotype

Homozygous: The snowcock mutation was induced by N-ethyl-N-nitrosourea (ENU)-mutagenesis and discovered in G3 mice screened for T-dependent (T-D) and T-independent (T-I) humoral responses. The Snowcock mice lack a T-dependent IgG response to OVA-Alum, a T-dependent IgG response to β-galactosidase (β-gal), and a T-independent IgM response to TNP-Ficoll. Flow cytometric analysis determined that the Snowcock mice have reduced numbers of CD4+ T cells and B cells.

Heterozygous: Not characterized.

Strain of Origin: C57BL/6J

Strain genetic background: C57BL/6J

Strain Development: Original mutant was a C57BL/6J G3 ENU-induced mutant; all subsequent crosses to maintain strain were on C57BL/6J background only.

Suggested Control Mice: Wild-type littermates or C57BL/6J mice

  • Cell Biology
  • Immunology and Inflammation
  • Models for Human Disease
    • B cell-negative severe combined immunodeficiency
  • Virology

Donor: Bruce Beutler , M.D., University of Texas Southwestern Medical Center.

Primary Reference:

Wang KW, Choi JH, Zeng M, Murray AR, and Beutler B. Record for snowcock. MUTAGENETIX™, B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.



Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Coat color: Black

Other:

MMRRC Breeding System: Random intra-strain mating

Breeding Scheme(s):

  • Wild-type female x Heterozygous male from the colony or reciprocal mating
  • Homozygous female x Homozygous male from the colony

Generation:

Overall Breeding Performance: Good

Viability and Fertility:FemaleMaleComments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Undetermined Undetermined

Average litter size: 4-6

Recommended wean age: 3-4 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.The donor or their institution limits the distribution to non-profit institutions only.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
- Products for this strain are Not Yet Available for Ordering -
If you register interest in this strain, you will be notified when it becomes available for ordering.
MMRRC Item # Description
Distribution
Fee/unit (US $)
Units Notes
037091-MU-RESUSLitter recovered from cryo-archive
$2,022.00
Non-Profit
Litter Recovered litter1; additional fees for any special requests.

1 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

3 An aliquot contains a sufficient number of embryos (in one or more vials and based on the transfer success rate of the MMRRC facility) to transfer to at least two recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

This strain is not currently available. If you register interest in this strain, you will be notified when it becomes available for ordering (click Register Interest button above).