Loading...

Strain Detail Sheet

Published: 06/16/2019
Revised: 08/04/2025

Strain Name:
B6.Cg-Tg(Amelx-Mmp20)42Jdb/Mmmh
Stock Number:
037131-MU
Citation ID:
RRID:MMRRC_037131-MU
Other Names:
Mmp20 Transgenic mice

Strain Information

Gene Details

Amelx
Name: amelogenin, X-linked
Synonyms: Amg, Amel, M100888, Rgsc888
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 11704
Homologene: 47996

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Mmp20
Name: matrix metallopeptidase 20 (enamelysin)
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 30800
VEGA: 9
HGNC: HGNC:7167
Homologene: 21001

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Tg(Amelx-Mmp20)42Jdb
Name: transgene insertion 42, John D Bartlett
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: UN
Alteration at locus: Transgenic
Genetic Alterations
The transgene vector was constructed so that mouse Mmp20 cDNA was placed between 4639 bp of the mouse amelogenin promoter and 1127 bp of amelogenin 3' non-coding region that included amelogenin polyadenylation sites. Founder 42 produces low levels of Mmp20 in incisors of mice that did not express the endogenous Mmp20 gene.
Genotype Determination
ES Cell Line
Not applicable

Strain Description

Phenotype

Homozygous: Decreased enamel hardness; low expression of the Mmp20 transgene.

Hemizygous: Not characterized.

MeSH Terms
  • Amelogenin/genetics
  • Amelogenin/metabolism
  • Animals
  • Blotting, Western
  • Dental Enamel/abnormalities
  • Dental Enamel/growth & development
  • Dental Enamel/ultrastructure
  • Dental Enamel Proteins/genetics
  • Dental Enamel Proteins/metabolism
  • Female
  • Hardness/physiology
  • Humans
  • Male
  • Matrix Metalloproteinase 20/physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microscopy, Electron, Scanning
  • Models, Animal
  • Phenotype
  • Promoter Regions, Genetic/genetics
  • RNA, Messenger/genetics
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tooth Calcification/physiology
  • X-Ray Microtomography
Strain GQC Summary
Gene Specific Genotyping:

To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

Background Genetic Quality:

The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

Suggested Control Mice
Littermates of all relevant genotypes.

Research Applications

  • Research Tools

Strain Origin

Donor
John Bartlett, Ph.D., Forsyth Institute.
Primary Reference
Shin M, Hu Y, Tye CE, Guan X, Deagle CC, Antone JV, Smith CE, Simmer JP, Bartlett JD. Matrix Metalloproteinase-20 Over-Expression Is Detrimental to Enamel Development: A Mus musculus Model. PLoS One. 2014 Jan 23;9(1):e86774. (Medline PMID:24466234)
Strain Development
Three transgenic founder mice were selected based on the ability of their incisor enamel organs to express low, intermediate, or high levels of Mmp20 transgene transcripts:

1) low- Tg(Amelx-Mmp20)42Jdb (this strain)
2) intermediate- Tg(Amelx-Mmp20)6Jdb, MMRRC:037100 B(Cg)-Tg(Amelx-Mmp20)6Jdb/MU
3) high- Tg(Amelx-Mmp20)24Jdb, MMRRC:037130 B6(Cg)-Tg(Amelx-Mmp20)24Jdb/Mmmh
After being backcrossed to C57BL/6 for over 8 generations, the mice were then bred to homozygosity through random intra-strain mating.

The donor also developed Mmp20 knockout, Tg positive mice (not held by the MMRRC). Investigators interested in using the same Mmp20 knockout, transgene-positive mouse appearing in the publication can review the following procedure:

Offspring carrying the low expression of the Mmp20 transgene transcripts are mated with Mmp20 knockout mice MMRRC:016999 B6;129P2-Mmp20tm1Jdb/Mmmh to produce heterozygous Tg positive Mmp20+/- mice. After being backcrossed to C57BL/6 for over 8 generations, the mice are then bred to homozygosity as STOCK Mmp20tm1Jdb Tg(Amelx-Mmp20)42Jdb mice through random intra-strain mating.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Health Status Report

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

Appearance

Coat Color
Agouti

Breeding

MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
  • Wild-type female x Hemizygous male from the colony or reciprocal mating
  • Hemizygous female x Hemizygous male from the colony
  • Hemizygous female x Homozygous male from the colony or reciprocal mating
  • Homozygous female x Homozygous male from the colony
Generation
N8+
Overall Breeding Performance
Good

Reproductive Statistics

Viability and Fertility: Female Male Comments
Homozygotes are viable: Yes Yes
Homozygotes are fertile: Yes Yes
Heterozygotes are fertile: Yes Yes
Age Reproductive Decline: Variable Variable
Average litter size
5-9
Recommended wean age
3-4 weeks

Order Information

Availability Level

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Conditions of Distribution

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Fees

- Products for this strain are Not Yet Available for Ordering
- If you register interest in this strain, you will be notified when it becomes available for ordering.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.