Strain Detail Sheet

Strain Name:
B6.Cg-Tg(Amelx-Mmp20)42Jdb/Mmmh
Stock Number:
037131-MU
Citation ID:
RRID:MMRRC_037131-MU
Other Names:
Mmp20 Transgenic mice

Strain Information

Gene Details

Amelx
Name: amelogenin, X-linked
Synonyms: Amg, Rgsc888, M100888, Amel
Type: Gene
Species: Mus musculus (mouse)
Chromosome: X
NCBI: 11704
Homologene: 47996
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Mmp20
Name: matrix metallopeptidase 20 (enamelysin)
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 30800
VEGA: 9
HGNC: HGNC:7167
Homologene: 21001
Genome Browser [Click image to go to Jbrowse]



Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Tg(Amelx-Mmp20)42Jdb
Name: transgene insertion 42, John D Bartlett
Type: Transgene
Species: Mus musculus (mouse)
Chromosome: UN
Alteration at locus: Transgenic
Genetic Alterations
Transgenic construct contains 4639 bp of 5' sequence of mouse Amelx promoter, 2278 bp Mmp20 cDNA, and 1127 bp of 3' sequence of Amelx which contains multiple transcription termination signals.
Genotype Determination
ES Cell Line
Not applicable

Strain Description

Phenotype

Homozygous: Decreased enamel hardness; low expression of the Mmp20 transgene.

Hemizygous: Not characterized.

MeSH Terms
  • Amelogenin/genetics
  • Amelogenin/metabolism
  • Animals
  • Blotting, Western
  • Dental Enamel/abnormalities
  • Dental Enamel/growth & development
  • Dental Enamel/ultrastructure
  • Dental Enamel Proteins/genetics
  • Dental Enamel Proteins/metabolism
  • Female
  • Hardness/physiology
  • Humans
  • Male
  • Matrix Metalloproteinase 20/physiology
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microscopy, Electron, Scanning
  • Models, Animal
  • Phenotype
  • Promoter Regions, Genetic/genetics
  • RNA, Messenger/genetics
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tooth Calcification/physiology
  • X-Ray Microtomography
Strain Development
Three transgenic founder mice were selected based on the ability of their incisor enamel organs to express low, intermediate, or high levels of Mmp20 transgene transcripts:
low- Tg(Amelx-Mmp20)42Jdb (this strain)
  • intermediate- Tg(Amelx-Mmp20)6Jdb, MMRRC:037100 B(Cg)-Tg(Amelx-Mmp20)6Jdb/MU
  • high- Tg(Amelx-Mmp20)24Jdb, MMRRC:037130 B6(Cg)-Tg(Amelx-Mmp20)24Jdb/Mmmh
    • After being backcrossed to C57BL/6 for over 8 generations, the mice were then bred to homozygosity through random intra-strain mating.

    The donor also developed Mmp20 knockout, Tg positive mice (not held by the MMRRC). Investigators interested in using the same Mmp20 knockout, transgene-positive mouse appearing in the publication can review the following procedure:
    Offspring carrying the low expression of the Mmp20 transgene transcripts were mated with Mmp20 knockout mice MMRRC:016999 B6;129P2-Mmp20tm1Jdb/Mmmh to produce heterozygous Tg positive Mmp20+/- mice. After being backcrossed to C57BL/6 for over 8 generations, the mice were then bred to homozygosity as STOCK Mmp20tm1Jdb Tg(Amelx-Mmp20)42Jdb mice through random intra-strain mating.
    Suggested Control Mice
    Wild-type littermates or C57BL/6 mice

    MMRRC Genetic QC

    MMRRC Genetic QC Summary
    The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.

    Research Applications

    • Research Tools

    Strain Origin

    Donor
    John Bartlett, Ph.D., Forsyth Institute.
    Primary Reference
    Shin M, Hu Y, Tye CE, Guan X, Deagle CC, Antone JV, Smith CE, Simmer JP, Bartlett JD. Matrix Metalloproteinase-20 Over-Expression Is Detrimental to Enamel Development: A Mus musculus Model. PLoS One. 2014 Jan 23;9(1):e86774. (Medline PMID:24466234)

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.

    Appearance

    Coat Color
    Agouti

    Breeding

    MMRRC Breeding System
    Random intra-strain mating
    Breeding Scheme(s)
    • Wild-type female x Hemizygous male from the colony or reciprocal mating
    • Hemizygous female x Hemizygous male from the colony
    • Hemizygous female x Homozygous male from the colony or reciprocal mating
    • Homozygous female x Homozygous male from the colony
    Generation
    N8+
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Yes Yes
    Homozygotes are fertile: Yes Yes
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Variable Variable
    Average litter size
    5-9
    Recommended wean age
    3-4 weeks

    Order Request Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    - Products for this strain are Not Yet Available for Ordering
    - If you register interest in this strain, you will be notified when it becomes available for ordering.
    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.