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The DNA construct contained in this transgenic mouse consists of a 33-kb BAC clone containing the human MGP gene, allowing the human MGP promoter to overexpress matrix gla proteinin multiple tissues.
Homozygous: There are less branches of pulmonary artery detected and inhibition of calcification in aortic tissue.The higher lethality of heterozygous MGPtg females led to difficulties in obtaining homozygous MGPtg mice. Because the phenotype was apparent in heterozygous MGPtg mice, these were used for characterization and the results from the three homozygous MGPtg mice were incorporated when available.
Hemizygous: There are less branches of pulmonary artery detected and inhibition of calcification in aortic tissue.The higher lethality of heterozygous MGPtg females led to difficulties in obtaining homozygous MGPtg mice. Because the phenotype was apparent in heterozygous MGPtg mice, these were used for characterization and the results from the three homozygous MGPtg mice were incorporated when available.
To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.
The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.
Yao Y, Nowak S, Yochelis A, Garfinkel A, Bostrom KI. Matrix GLA protein, an inhibitory morphogen in pulmonary vascular development. J Biol Chem. 2007 Oct 12;282(41):30131-42. Epub 2007 Aug 1. (Medline PMID: 17670744)
Yao Y, Bennett BJ, Wang X, Rosenfeld ME, Giachelli C, Lusis AJ, Bostrom KI. Inhibition of bone morphogenetic proteins protects against atherosclerosis and vascular calcification. Circ Res. 2010 Aug 20;107(4):485-94. Epub 2010 Jun 24. (Medline PMID: 20576934)
Bostrom KI, Jumabay M, Matveyenko A, Nicholas SB, Yao Y. Activation of vascular bone morphogenetic protein signaling in diabetes mellitus. Circ Res. 2011 Feb 18;108(4):446-57. Epub 2010 Dec 30. (Medline PMID: 21193740)
Yao Y, Jumabay M, Wang A, Bostrom KI.Matrix Gla protein deficiency causes arteriovenous malformations in mice. J Clin Invest. 2011 Aug;121(8):2993-3004. Epub 2011 Jul 18. (Medline PMID: 21765215)
Yao Y, Jumabay M, Ly A, Radparvar M, Cubberly MR, Bostrom KI. A role for the endothelium in vascular calcification. Circ Res. 2013 Aug 16;113(5):495-504. Epub 2013 Jul 12. (Medline PMID: 23852538)
Transgenic mice over-expressing matrix gla protein, MGP, in multiple organs were generated by injection into C57BL/6 oocytes. Founder mice were crossed to wild-type C57BL/6 mice to generate F1 heterozygous mice. Subsequent generations of transgenic mice were obtained by sib-mating.
Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain's tissue samples taken when archived by or ordered from the assigned MMRRC Center.
Colony Surveillance Program and Current Health Reports
Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.
Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.
Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.
1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.
3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.
4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.
To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.
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