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Strain Detail Sheet

Strain Name:
B6J.B6N-Ube3atm1a(KOMP)Wtsi/Mmnc
Stock Number:
037375-UNC
Citation ID:
RRID:MMRRC_037375-UNC
Other Names:
Ube3atm1a(KOMP)Wtsi, Ube3a Knockout First

Strain Information

Gene Details

Ube3atm1a(KOMP)Wtsi
Name: ubiquitin protein ligase E3A; targeted mutation 1a, Wellcome Trust Sanger Institute
Type: Allele
Species: Mus musculus (mouse)
Chromosome: 7
Alteration at locus: Knockout-first
Ube3a
Name: ubiquitin protein ligase E3A
Synonyms: Hpve6a, E6-AP ubiquitin protein ligase, A130086L21Rik, 5830462N02Rik
Type: Gene
Species: Mouse
Chromosome: 7
Alteration at locus: Knockout-first
NCBI: 22215
Homologene: 7988

Additional Resources
IMPC
Google
Entrez
PubMed
IMSR
Gene Ontology
BioGPS
Gene Cloud
IGTC
Mouse Phenome DB
Mouse Mine
KOMP Phenotyping
Monarch
GeneCards
ClinGen
Genetic Alterations
A LacZ gene-trap cassette was knocked into the endogenous Ube3a locus via homologous recombination in C57BL6 mouse ES cells. This allele is known as a "knockout First allele ", as it is initially non-expressive, but can be converted to a conditional allele via Flp recombination. Germline chimeric males (determined by the transmission of coat color in parallel breeding) were then crossed to C57BL/6 female homozygous Rosa26-FLPe mice (B6.129S4-Gt(ROSA)26Sortm1(FLP1)Dym/RainJ, Jackson Stock #009086) in order to excise the FRT-flanked lacZ gene trap from the Ube3aKO1st allele and thereby produce the Ube3aFLOX allele.
Genotype Determination
  • Genotyping Protocol(s)
  • Center protocol and contact for technical support will be shipped with mice.
    • ES Cell Line
    C57BL/6N-PRX-B6N #1 derived from C57BL/6N

    Strain Description

    Phenotype

    Homozygous: unknown

    Hetero/Hemizygous: Mice are viable and fertile and are obtained at mendelian ratios regardless of parental inheritance of the allele. When the inherited allele is maternally inherited, Ube3a protein expression in the brain is drastically reduced. Cellular, physiological, and behavioral phenotypes have yet to be explored.

    Cre-excised Phenotype: N/A

    Strain GQC Summary
    Gene Specific Genotyping:

    To request gene-specific and other genotyping services for a strain, please contact the distribution MMRRC Center for more information.

    Background Genetic Quality:

    The MMRRC has developed a Genetic Quality Control pipeline using the MiniMUGA array to provide additional information to identify and validate genetic backgrounds of MMRRC strains. For more information on whether genetic background data is available, please contact MMRRC_GeneticQC@med.unc.edu. Note: that MiniMUGA genetic background data is not available on all strains, but can be ordered if desired.

    Suggested Control Mice
    Littermates of all relevant genotypes.

    Research Applications

    • Cell Biology
    • Developmental Biology
    • Models for Human Disease
    • Neurobiology

    Strain Origin

    Donor
    Benjamin Philpot, Ph.D., UNC-Chapel Hill.
    Strain Development
    C57BL/6N germline male chimeras were bred to C57BL/6J wild type females, resulting in heterozygous Ube3a knockout first females, which were in turn bred to a wild type C57BL/6J wild type male. One of the heterozygous male offspring was mated to a C57BL/6J wild type female to produce the heterozygous male mice to be donated.


    Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

    Colony and Husbandry Information

    Health Status Report

    Colony Surveillance Program and Current Health Reports

    Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc_health@med.unc.edu.

    Appearance

    Coat Color
    black

    Breeding

    MMRRC Breeding System
    Backcross
    Generation
    Total of N=7 and F=3 generations from this colony. 
    Overall Breeding Performance
    Good

    Reproductive Statistics

    Viability and Fertility: Female Male Comments
    Homozygotes are viable: Unknown Unknown
    Homozygotes are fertile: Unknown Unknown
    Heterozygotes are fertile: Yes Yes
    Age Reproductive Decline: Unknown Unknown
    Bred to Homozygosity
    No
    Average litter size
    5-9
    Recommended wean age
    3 weeks

    Order Information

    Availability Level

    Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    Conditions of Distribution

    Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

    The donor or their institution limits the distribution to non-profit institutions only.

    Fees

    Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

    Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
    MMRRC Item # Description Distribution Fee / Unit (US $)
    *Shipping & Handling not included*    		 Units Notes
    037375-UNC-SPERM Cryo-preserved spermatozoa $520.00 / Non-Profit Aliquot Approximate quantity3
    037375-UNC-RESUS Litter recovered from cryo-archive $3,242.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
    Cryopreserved material may be available upon request, please inquire to mmrrc@med.unc.edu for more information.

    1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

    3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

    4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

    To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.