Strain Name:
C57BL/6J-MtgxP0027Btlr/Mmmh
Stock Number:
038280-MU
Citation ID:
RRID:MMRRC_038280-MU
Other Names:
P0027 (G1), C57BL/6J-MtgxP0027Btlr
Major Collection:

Strain Information

Msi2
Name: musashi RNA-binding protein 2
Synonyms: msi2h, Musashi2
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 76626
Homologene: 62199
Nup188
Name: nucleoporin 188
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 227699
Homologene: 45683
Lims1
Name: LIM and senescent cell antigen-like domains 1
Synonyms: PINCH1, Lims1l, 4921524A02Rik, C430041B13Rik, 2310016J22Rik
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 110829
HGNC: HGNC:6616
Homologene: 68428
Copa
Name: coatomer protein complex subunit alpha
Synonyms: xenin
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 12847
HGNC: HGNC:2230
Homologene: 3218
Bicd2
Name: BICD cargo adaptor 2
Synonyms: 0610027D24Rik, 1110005D12Rik
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 76895
VEGA: 13
Homologene: 9070
Phactr4
Name: phosphatase and actin regulator 4
Synonyms: 3110001B12Rik, C330013F19Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 100169
Homologene: 41537
Kdm2a
Name: lysine (K)-specific demethylase 2A
Synonyms: Cxxc8, Fbxl11, Jhdm1a, Fbl7, lalina, 5530401A10Rik, Gm4560
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 225876
Homologene: 56564
Ftsj3
Name: FtsJ RNA 2'-O-methyltransferase 3
Synonyms: D11Ertd400e, Epcs3, C79843
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 56095
Homologene: 5451
Marco
Name: macrophage receptor with collagenous structure
Synonyms: Scara2
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 17167
HGNC: HGNC:6895
Homologene: 4928
Or1n2
Name: olfactory receptor family 1 subfamily N member 2
Synonyms: GA_x6K02T2NLDC-33601476-33602429, Olfr354, MOR127-4
Type: Gene
Species: Mouse
Chromosome: 2
NCBI: 258941
Homologene: 17443
Nap1l5
Name: nucleosome assembly protein 1-like 5
Synonyms: 1110020M21Rik
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 58243
Homologene: 36397
Yif1b
Name: Yip1 interacting factor homolog B (S. cerevisiae)
Synonyms: 9430029K10Rik
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 77254
Homologene: 60114
Casp1
Name: caspase 1
Synonyms: Il1bc, Caspase-1, interleukin 1 beta-converting enzyme, ICE
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 12362
VEGA: 9
HGNC: HGNC:1499
Homologene: 133272
Tmem26
Name: transmembrane protein 26
Type: Gene
Species: Mouse
Chromosome: 10
NCBI: 327766
VEGA: 10
Homologene: 14428
Sec14l2
Name: SEC14-like lipid binding 2
Synonyms: 1300013M05Rik, tap, Spf
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 67815
Homologene: 8245
Camta2
Name: calmodulin binding transcription activator 2
Synonyms: Kiaa0909-hp
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 216874
Homologene: 9021
Tent4a
Name: terminal nucleotidyltransferase 4A
Synonyms: TRF4-1, Papd7, TRF4, Pols, LAK-1
Type: Gene
Species: Mouse
Chromosome: 13
NCBI: 210106
Homologene: 5089
Myh15
Name: myosin, heavy chain 15
Synonyms: EG667772
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 667772
VEGA: 16
Homologene: 18929
Sim2
Name: single-minded family bHLH transcription factor 2
Synonyms: bHLHe15
Type: Gene
Species: Mouse
Chromosome: 16
NCBI: 20465
VEGA: 16
Homologene: 3716
Klhl14
Name: kelch-like 14
Synonyms: 6330403N15Rik, printor
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 225266
Homologene: 28121
Ms4a10
Name: membrane-spanning 4-domains, subfamily A, member 10
Synonyms: 2010001N17Rik
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 69826
Homologene: 11355
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to T, chromosome 1 at 120,474,712 bp
  • A to G, chromosome 1 at 172,111,948 bp
  • A to G, chromosome 2 at 30,322,681 bp
  • T to C, chromosome 2 at 36,907,570 bp
  • G to C, chromosome 4 at 132,371,090 bp
  • T to A, chromosome 6 at 58,906,825 bp
  • T to C, chromosome 7 at 29,238,613 bp
  • A to T, chromosome 9 at 5,299,851 bp
  • A to G, chromosome 10 at 58,418,455 bp
  • A to G, chromosome 10 at 68,778,718 bp
  • C to T, chromosome 11 at 4,103,673 bp
  • A to G, chromosome 11 at 70,684,005 bp
  • A to T, chromosome 11 at 88,394,597 bp
  • C to A, chromosome 11 at 106,254,808 bp
  • C to A, chromosome 13 at 49,379,651 bp
  • G to A, chromosome 13 at 69,506,955 bp
  • C to T, chromosome 16 at 49,081,208 bp
  • C to A, chromosome 16 at 94,109,422 bp
  • T to C, chromosome 18 at 21,558,135 bp
  • C to T, chromosome 19 at 4,343,245 bp
  • T to C, chromosome 19 at 10,964,128 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
MMRRC Genetic QC Summary
The MMRRC Centers have developed a genetic QC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC congenic and inbred strains. For more information on when data may be available, or to request genotyping for a strain of interest, please contact mmrrc@missouri.edu. Older strains may not have this information.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for P0027 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038280-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.