Strain Name:
C57BL/6J-MtgxP0035Btlr/Mmmh
Stock Number:
038285-MU
Citation ID:
RRID:MMRRC_038285-MU
Other Names:
P0035 (G1), C57BL/6J-MtgxP0035Btlr
Major Collection:

Gene Information

Med13l
Name: mediator complex subunit 13-like
Synonyms: Trap240L, 2210413I17Rik, 9030618F05Rik, 6330591G05Rik, Thrap2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 76199
Homologene: 25256
Brd4
Name: bromodomain containing 4
Synonyms: MCAP, WI-11513, HUNK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 57261
Homologene: 136213
Zfp462
Name: zinc finger protein 462
Synonyms: 9430078C22Rik, Zfpip, Gt4-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 242466
Homologene: 41430
Mdn1
Name: midasin AAA ATPase 1
Synonyms: 4833432B22Rik, D4Abb1e, LOC213784
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 100019
Homologene: 39689
Pggt1b
Name: protein geranylgeranyltransferase type I, beta subunit
Synonyms: BGG1, 2010207C17Rik, GGT1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 225467
VEGA: 18
HGNC: HGNC:8895
Homologene: 3685
Htr2b
Name: 5-hydroxytryptamine (serotonin) receptor 2B
Synonyms: 5-HT2B
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 15559
HGNC: HGNC:5294
Homologene: 55492
Bub1b
Name: BUB1B, mitotic checkpoint serine/threonine kinase
Synonyms: BUBR1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 12236
HGNC: HGNC:1149
Homologene: 933
Tbc1d32
Name: TBC1 domain family, member 32
Synonyms: D630037F22Rik, b2b2284Clo, Bromi, C6orf170
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 544696
VEGA: 10
Homologene: 51889
Scfd2
Name: Sec1 family domain containing 2
Synonyms: STXBP1L1, E430013M20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 212986
Homologene: 77411
Dsg3
Name: desmoglein 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 13512
VEGA: 18
HGNC: HGNC:3050
Homologene: 55513
Cfap70
Name: cilia and flagella associated protein 70
Synonyms: Ttc18, 5330402L21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 76670
Homologene: 17048
Pkhd1
Name: polycystic kidney and hepatic disease 1
Synonyms: FPC, tigmin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241035
HGNC: HGNC:9016
Homologene: 16336
Cryba2
Name: crystallin, beta A2
Synonyms: E130107M19Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12958
HGNC: HGNC:2395
Homologene: 10991
Pcsk2
Name: proprotein convertase subtilisin/kexin type 2
Synonyms: prohormone convertase 2, Phpp-2, 6330411F23Rik, Nec-2, Nec2, SPC2, PC2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 18549
HGNC: HGNC:8744
Homologene: 37640
Lmod2
Name: leiomodin 2 (cardiac)
Synonyms: C-Lmod
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 93677
HGNC: HGNC:6648
Homologene: 28302
Lrrc37a
Name: leucine rich repeat containing 37A
Synonyms: LOC237954
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 237954
Homologene: 138824
Ttc8
Name: tetratricopeptide repeat domain 8
Synonyms: 0610012F22Rik, BBS8
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 76260
Homologene: 14988
Muc4
Name: mucin 4
Synonyms: 4933405I11Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 16
NCBI: 140474
HGNC: HGNC:7514
Homologene: 124469
Psd
Name: pleckstrin and Sec7 domain containing
Synonyms: Efa6a, Psdl, 1110007H17Rik, Efa6
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 73728
VEGA: 19
HGNC: HGNC:9507
Homologene: 31115
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 20,117,347 bp
  • A to G, chromosome 1 at 74,890,012 bp
  • A to T, chromosome 1 at 86,110,730 bp
  • G to A, chromosome 2 at 118,622,185 bp
  • C to T, chromosome 2 at 143,795,951 bp
  • A to T, chromosome 4 at 32,749,934 bp
  • T to C, chromosome 4 at 55,009,086 bp
  • A to T, chromosome 5 at 74,225,319 bp
  • C to T, chromosome 5 at 118,742,620 bp
  • T to C, chromosome 6 at 24,597,886 bp
  • A to C, chromosome 10 at 56,198,439 bp
  • T to A, chromosome 11 at 103,503,132 bp
  • T to C, chromosome 12 at 98,976,416 bp
  • A to G, chromosome 14 at 20,424,471 bp
  • T to C, chromosome 16 at 32,760,248 bp
  • C to T, chromosome 17 at 32,212,838 bp
  • A to C, chromosome 18 at 20,539,969 bp
  • G to C, chromosome 18 at 46,259,720 bp
  • T to C, chromosome 19 at 46,320,961 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for P0035 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038285-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.