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Strain Name:
C57BL/6J-MtgxR0001Btlr/Mmmh
Stock Number:
038297-MU
Citation ID:
RRID:MMRRC_038297-MU
Other Names:
R0001 (G1), C57BL/6J-MtgxR0001Btlr
Major Collection:

Strain Information

Pknox1
Name: Pbx/knotted 1 homeobox
Synonyms: PREP1, D17Wsu76e
Type: Gene
Species: Mouse
Chromosome: 17
NCBI: 18771
HGNC: HGNC:9022
Homologene: 3363
Epha7
Name: Eph receptor A7
Synonyms: MDK1, Ebk, Cek11, Ehk3, Hek11, Mdk1
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 13841
HGNC: HGNC:3390
Homologene: 20935
Rad54l2
Name: RAD54 like 2 (S. cerevisiae)
Synonyms: Arip4, G630026H09Rik, Srisnf2l
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 81000
Homologene: 56698
Jak2
Name: Janus kinase 2
Synonyms: C81284
Type: Gene
Species: Mouse
Chromosome: 19
NCBI: 16452
VEGA: 19
HGNC: HGNC:6192
Homologene: 21033
Dhx9
Name: DExH-box helicase 9
Synonyms: leukophysin, nuclear DNA helicase II, RNA helicase, Ddx9, NDH II, NDHII, RHA
Type: Gene
Species: Mouse
Chromosome: 1
NCBI: 13211
HGNC: HGNC:2750
Homologene: 1039
Lig3
Name: ligase III, DNA, ATP-dependent
Synonyms: D11Wsu78e
Type: Gene
Species: Mouse
Chromosome: 11
NCBI: 16882
HGNC: HGNC:6600
Homologene: 32109
Trappc9
Name: trafficking protein particle complex 9
Synonyms: 4632408O18Rik, 2900005P22Rik, Nibp, TRS130, 1810044A24Rik
Type: Gene
Species: Mouse
Chromosome: 15
NCBI: 76510
VEGA: 15
Homologene: 81931
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 1 at 43,971,726 bp
  • A to T, chromosome 1 at 81,192,107 bp
  • A to G, chromosome 1 at 92,573,461 bp
  • A to T, chromosome 1 at 131,168,127 bp
  • A to G, chromosome 1 at 135,272,485 bp
  • A to T, chromosome 1 at 136,282,888 bp
  • G to T, chromosome 1 at 153,462,636 bp
  • C to T, chromosome 1 at 154,205,286 bp
  • A to G, chromosome 2 at 76,776,972 bp
  • G to A, chromosome 2 at 76,832,089 bp
  • A to C, chromosome 2 at 79,326,587 bp
  • A to G, chromosome 2 at 120,318,053 bp
  • C to A, chromosome 2 at 120,723,751 bp
  • G to T, chromosome 2 at 121,077,646 bp
  • G to A, chromosome 2 at 122,125,710 bp
  • A to G, chromosome 2 at 164,948,383 bp
  • T to C, chromosome 2 at 180,984,412 bp
  • T to A, chromosome 2 at 181,574,655 bp
  • T to G, chromosome 3 at 122,081,011 bp
  • A to T, chromosome 3 at 142,828,988 bp
  • T to C, chromosome 4 at 28,961,279 bp
  • G to A, chromosome 4 at 58,066,460 bp
  • T to A, chromosome 4 at 139,451,788 bp
  • T to A, chromosome 5 at 31,495,617 bp
  • C to T, chromosome 5 at 114,204,833 bp
  • T to A, chromosome 5 at 114,260,870 bp
  • A to G, chromosome 5 at 148,921,275 bp
  • T to C, chromosome 6 at 46,530,171 bp
  • G to A, chromosome 6 at 58,265,717 bp
  • T to A, chromosome 7 at 12,554,607 bp
  • T to A, chromosome 7 at 16,793,637 bp
  • G to A, chromosome 7 at 43,636,876 bp
  • A to T, chromosome 7 at 76,419,863 bp
  • T to A, chromosome 7 at 104,179,473 bp
  • C to T, chromosome 7 at 135,699,172 bp
  • T to A, chromosome 7 at 135,701,019 bp
  • T to C, chromosome 7 at 141,641,574 bp
  • A to T, chromosome 8 at 22,158,612 bp
  • A to T, chromosome 8 at 25,859,270 bp
  • C to A, chromosome 8 at 109,628,633 bp
  • T to C, chromosome 8 at 122,478,920 bp
  • T to C, chromosome 8 at 122,595,883 bp
  • T to C, chromosome 9 at 16,377,873 bp
  • C to A, chromosome 9 at 46,242,892 bp
  • A to G, chromosome 9 at 106,708,217 bp
  • T to C, chromosome 9 at 107,742,424 bp
  • G to T, chromosome 10 at 6,900,600 bp
  • G to A, chromosome 10 at 80,259,061 bp
  • A to G, chromosome 10 at 102,388,956 bp
  • G to T, chromosome 10 at 117,074,876 bp
  • T to A, chromosome 11 at 51,268,765 bp
  • G to A, chromosome 11 at 54,028,003 bp
  • A to G, chromosome 11 at 59,373,180 bp
  • A to G, chromosome 11 at 71,161,759 bp
  • C to T, chromosome 11 at 82,790,591 bp
  • C to T, chromosome 12 at 57,460,839 bp
  • A to T, chromosome 12 at 77,475,315 bp
  • A to G, chromosome 13 at 100,214,650 bp
  • C to A, chromosome 13 at 100,223,114 bp
  • T to C, chromosome 13 at 112,964,556 bp
  • A to G, chromosome 14 at 24,452,189 bp
  • A to G, chromosome 14 at 63,135,622 bp
  • C to A, chromosome 14 at 122,478,957 bp
  • A to T, chromosome 15 at 72,963,662 bp
  • A to G, chromosome 15 at 83,228,289 bp
  • A to G, chromosome 15 at 88,600,909 bp
  • C to T, chromosome 15 at 89,336,274 bp
  • A to T, chromosome 16 at 10,514,433 bp
  • A to T, chromosome 17 at 31,599,636 bp
  • T to C, chromosome 17 at 34,061,612 bp
  • A to T, chromosome 18 at 37,423,989 bp
  • C to T, chromosome 18 at 43,358,375 bp
  • T to C, chromosome 18 at 44,107,696 bp
  • T to C, chromosome 18 at 49,888,897 bp
  • A to T, chromosome 19 at 22,715,331 bp
  • A to G, chromosome 19 at 29,282,387 bp
  • T to A, chromosome 19 at 57,381,756 bp
  • G to A, chromosome 19 at 58,789,171 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0001 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038297-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.