Strain Name:
C57BL/6J-MtgxR0002Btlr/Mmmh
Stock Number:
038298-MU
Citation ID:
RRID:MMRRC_038298-MU
Other Names:
R0002 (G1), C57BL/6J-MtgxR0002Btlr
Major Collection:

Gene Information

Slc26a5
Name: solute carrier family 26, member 5
Synonyms: prestin, Pres
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 80979
HGNC: HGNC:9359
Homologene: 69472
Tacc2
Name: transforming, acidic coiled-coil containing protein 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 57752
Homologene: 5087
Bcl2
Name: B cell leukemia/lymphoma 2
Synonyms: D830018M01Rik, C430015F12Rik, Bcl-2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 12043
HGNC: HGNC:990
Homologene: 527
Icam5
Name: intercellular adhesion molecule 5, telencephalin
Synonyms: Icam3, Tlcn, TLN, CD50
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 15898
VEGA: 9
HGNC: HGNC:5348
Homologene: 2447
Aco1
Name: aconitase 1
Synonyms: Irp1, Aco-1, Irebp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 11428
HGNC: HGNC:117
Homologene: 1657
Ubr4
Name: ubiquitin protein ligase E3 component n-recognin 4
Synonyms: D930005K06Rik, p600, LOC381562, 1810009A16Rik, A930005E13Rik, Zubr1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 69116
Homologene: 10804
Chn2
Name: chimerin 2
Synonyms: 1700026N20Rik, 4930557O16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 69993
HGNC: HGNC:1944
Homologene: 31213
Lgr6
Name: leucine-rich repeat-containing G protein-coupled receptor 6
Synonyms: A530037C04Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 329252
Homologene: 49680
Cdhr5
Name: cadherin-related family member 5
Synonyms: 1810074H01Rik, Mucdhl, Mupcdh
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 72040
HGNC: HGNC:7521
Homologene: 69345
Ncoa1
Name: nuclear receptor coactivator 1
Synonyms: SRC-1, SRC-a/NCoA-1, bHLHe74, SRC1, steroid receptor coactivator-1, KAT13A
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 17977
VEGA: 12
HGNC: HGNC:7668
Homologene: 7859
Prkag3
Name: protein kinase, AMP-activated, gamma 3 non-catalytic subunit
Synonyms: AMPKg3S, AMPKg3, AMPKg3L
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 241113
HGNC: HGNC:9387
Homologene: 23006
Patl2
Name: protein associated with topoisomerase II homolog 2 (yeast)
Synonyms: 4930424G05Rik, Pat1a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 67578
Homologene: 12155
Spink12
Name: serine peptidase inhibitor, Kazal type 12
Synonyms: 9230117E20Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 78242
Homologene: 87071
Tnr
Name: tenascin R
Synonyms: janusin, restrictin, TN-R, J1-tenascin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 21960
Homologene: 124416
F5
Name: coagulation factor V
Synonyms: Cf-5, Cf5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 1
NCBI: 14067
HGNC: HGNC:3542
Homologene: 104
Dhx36
Name: DEAH (Asp-Glu-Ala-His) box polypeptide 36
Synonyms: Ddx36, 2810407E23Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 3
NCBI: 72162
Homologene: 6356
Gm9954
Name: predicted gene 9954
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
Bri3
Name: brain protein I3
Synonyms: I3 protein
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 55950
HGNC: HGNC:1109
Homologene: 9114
Tas2r113
Name: taste receptor, type 2, member 113
Synonyms: mGR13, mt2r58, Tas2r13, T2R13
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 387345
Homologene: 130075
Pik3c2g
Name: phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 gamma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 18705
HGNC: HGNC:8973
Homologene: 3362
Sbk3
Name: SH3 domain binding kinase family, member 3
Synonyms: LOC381835, Gm1078
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 381835
Homologene: 82595
Hsh2d
Name: hematopoietic SH2 domain containing
Synonyms: Hsh2, ALX
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 209488
Homologene: 13142
Zfp560
Name: zinc finger protein 560
Synonyms: 2310030G09Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 434377
Homologene: 103820
Col5a3
Name: collagen, type V, alpha 3
Synonyms: Pro-alpha3(V)
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 53867
Homologene: 9253
Pate14
Name: prostate and testis expressed 14
Synonyms: A630095E13Rik, LOC235973, Sslp-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 235973
Exph5
Name: exophilin 5
Synonyms: Slac2b, AC079869.22gm5, slac2-b, B130009M24Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 320051
Homologene: 9007
Iqch
Name: IQ motif containing H
Synonyms: 4921504K03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 78250
Homologene: 11258
Itga9
Name: integrin alpha 9
Synonyms: 6720458D17Rik, 2610002H11Rik, D9Ertd428e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 104099
HGNC: HGNC:6145
Homologene: 1664
Sult3a2
Name: sulfotransferase family 3A, member 2
Synonyms: Gm4794
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 215895
VEGA: 10
Homologene: 111031
Nuak1
Name: NUAK family, SNF1-like kinase, 1
Synonyms: B230104P22Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 77976
Homologene: 8896
Ccdc125
Name: coiled-coil domain containing 125
Synonyms: 5830436D01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 76041
VEGA: 13
Homologene: 27932
Tapbp
Name: TAP binding protein
Synonyms: D17Wsu91e, TPN, tapasin
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 21356
Homologene: 2401
Catsper1
Name: cation channel, sperm associated 1
Synonyms: KSper
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 225865
VEGA: 19
Homologene: 14207
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 74,744,788 bp
  • T to C, chromosome 1 at 106,712,511 bp
  • C to T, chromosome 1 at 134,994,010 bp
  • T to C, chromosome 1 at 159,874,200 bp
  • T to C, chromosome 1 at 164,201,631 bp
  • G to A, chromosome 2 at 122,125,710 bp
  • A to C, chromosome 3 at 62,480,839 bp
  • T to C, chromosome 4 at 40,176,649 bp
  • T to C, chromosome 4 at 139,390,900 bp
  • T to A, chromosome 5 at 21,814,983 bp
  • G to T, chromosome 5 at 63,650,235 bp
  • G to T, chromosome 5 at 144,244,502 bp
  • A to G, chromosome 6 at 54,273,113 bp
  • T to A, chromosome 6 at 132,893,779 bp
  • A to G, chromosome 6 at 139,768,745 bp
  • T to C, chromosome 7 at 4,970,631 bp
  • T to A, chromosome 7 at 130,621,785 bp
  • G to T, chromosome 7 at 141,270,020 bp
  • G to A, chromosome 8 at 72,200,460 bp
  • T to C, chromosome 9 at 20,347,517 bp
  • T to A, chromosome 9 at 20,809,856 bp
  • T to A, chromosome 9 at 21,033,505 bp
  • A to T, chromosome 9 at 36,637,359 bp
  • G to T, chromosome 9 at 53,373,956 bp
  • T to C, chromosome 9 at 63,594,743 bp
  • G to A, chromosome 9 at 118,843,603 bp
  • T to C, chromosome 10 at 33,779,807 bp
  • A to T, chromosome 10 at 84,375,367 bp
  • A to G, chromosome 12 at 4,290,885 bp
  • C to T, chromosome 13 at 100,693,606 bp
  • C to T, chromosome 17 at 33,925,632 bp
  • T to C, chromosome 18 at 44,107,696 bp
  • A to G, chromosome 19 at 5,341,523 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0002 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038298-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.