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Strain Name:
C57BL/6J-MtgxR0003Btlr/Mmmh
Stock Number:
038299-MU
Citation ID:
RRID:MMRRC_038299-MU
Other Names:
R0003 (G1), C57BL/6J-MtgxR0003Btlr
Major Collection:

Strain Information

Nfat5
Name: nuclear factor of activated T cells 5
Synonyms: nfatz, TonEBP, B130038B15Rik, OREBP
Type: Gene
Species: Mouse
Chromosome: 8
NCBI: 54446
HGNC: HGNC:7774
Homologene: 4811
Camk2a
Name: calcium/calmodulin-dependent protein kinase II alpha
Synonyms: alpha-CaMKII
Type: Gene
Species: Mouse
Chromosome: 18
NCBI: 12322
HGNC: HGNC:1460
Homologene: 56577
Glrb
Name: glycine receptor, beta subunit
Type: Gene
Species: Mouse
Chromosome: 3
NCBI: 14658
HGNC: HGNC:4329
Homologene: 20224
Pik3cd
Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
Synonyms: p110delta, 2410099E07Rik, 2610208K16Rik
Type: Gene
Species: Mouse
Chromosome: 4
NCBI: 18707
HGNC: HGNC:8977
Homologene: 3686
Ccdc12
Name: coiled-coil domain containing 12
Synonyms: 2700094L05Rik
Type: Gene
Species: Mouse
Chromosome: 9
NCBI: 72654
Homologene: 41751
Exoc6b
Name: exocyst complex component 6B
Synonyms: 4930569O18Rik, G430127E12Rik, Sec15b, Sec15l2
Type: Gene
Species: Mouse
Chromosome: 6
NCBI: 75914
Homologene: 44781
Dock1
Name: dedicator of cytokinesis 1
Synonyms: D630004B07Rik, 9130006G06Rik, Dock180, b2b3190Clo
Type: Gene
Species: Mouse
Chromosome: 7
NCBI: 330662
HGNC: HGNC:2987
Homologene: 55575
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • T to C, chromosome 1 at 36,524,043 bp
  • T to A, chromosome 1 at 43,960,139 bp
  • T to C, chromosome 1 at 60,387,126 bp
  • A to C, chromosome 1 at 153,262,439 bp
  • A to T, chromosome 1 at 174,205,273 bp
  • A to T, chromosome 1 at 179,763,473 bp
  • C to A, chromosome 1 at 181,114,133 bp
  • T to C, chromosome 1 at 185,414,391 bp
  • T to A, chromosome 1 at 188,578,491 bp
  • T to C, chromosome 2 at 76,743,683 bp
  • T to A, chromosome 2 at 87,002,366 bp
  • C to T, chromosome 2 at 91,911,428 bp
  • G to A, chromosome 2 at 109,997,665 bp
  • G to A, chromosome 2 at 120,618,968 bp
  • T to C, chromosome 2 at 131,075,060 bp
  • T to G, chromosome 2 at 180,178,079 bp
  • C to A, chromosome 2 at 180,583,438 bp
  • G to A, chromosome 2 at 181,801,871 bp
  • T to A, chromosome 3 at 80,855,914 bp
  • T to C, chromosome 3 at 90,119,911 bp
  • C to A, chromosome 3 at 90,408,511 bp
  • A to G, chromosome 3 at 96,684,015 bp
  • A to G, chromosome 3 at 107,604,976 bp
  • A to T, chromosome 4 at 11,267,619 bp
  • T to C, chromosome 4 at 25,007,651 bp
  • A to G, chromosome 4 at 40,695,145 bp
  • A to T, chromosome 4 at 43,970,532 bp
  • A to T, chromosome 4 at 89,151,998 bp
  • T to C, chromosome 4 at 146,537,760 bp
  • A to G, chromosome 4 at 149,656,379 bp
  • A to G, chromosome 5 at 45,555,194 bp
  • G to T, chromosome 5 at 57,913,248 bp
  • T to A, chromosome 5 at 114,398,851 bp
  • T to G, chromosome 5 at 121,407,184 bp
  • G to A, chromosome 5 at 135,133,189 bp
  • G to T, chromosome 6 at 56,736,483 bp
  • G to T, chromosome 6 at 67,316,286 bp
  • A to G, chromosome 6 at 84,854,699 bp
  • T to A, chromosome 6 at 85,629,210 bp
  • A to G, chromosome 7 at 6,676,948 bp
  • G to T, chromosome 7 at 15,971,887 bp
  • T to C, chromosome 7 at 19,519,927 bp
  • T to C, chromosome 7 at 24,989,564 bp
  • A to G, chromosome 7 at 43,355,926 bp
  • C to T, chromosome 7 at 44,715,409 bp
  • T to C, chromosome 7 at 57,413,728 bp
  • T to C, chromosome 7 at 68,165,242 bp
  • T to C, chromosome 7 at 102,877,861 bp
  • T to C, chromosome 7 at 102,954,978 bp
  • G to T, chromosome 7 at 129,599,061 bp
  • T to C, chromosome 7 at 134,730,064 bp
  • A to G, chromosome 8 at 25,568,198 bp
  • A to T, chromosome 8 at 60,927,296 bp
  • A to T, chromosome 8 at 71,708,953 bp
  • A to C, chromosome 8 at 104,628,667 bp
  • A to C, chromosome 8 at 105,395,361 bp
  • C to T, chromosome 8 at 107,339,075 bp
  • G to A, chromosome 8 at 123,456,495 bp
  • A to G, chromosome 9 at 38,473,316 bp
  • A to G, chromosome 9 at 110,656,597 bp
  • A to G, chromosome 10 at 39,060,222 bp
  • A to T, chromosome 11 at 32,589,460 bp
  • T to A, chromosome 11 at 46,128,789 bp
  • T to A, chromosome 11 at 49,867,039 bp
  • T to A, chromosome 11 at 78,286,578 bp
  • G to T, chromosome 11 at 89,015,772 bp
  • A to G, chromosome 11 at 106,301,520 bp
  • A to T, chromosome 11 at 114,928,338 bp
  • A to G, chromosome 11 at 115,655,425 bp
  • A to T, chromosome 11 at 121,943,673 bp
  • A to C, chromosome 12 at 36,130,015 bp
  • T to A, chromosome 12 at 75,632,593 bp
  • C to A, chromosome 13 at 11,824,379 bp
  • C to T, chromosome 13 at 67,098,469 bp
  • T to C, chromosome 13 at 96,652,145 bp
  • T to A, chromosome 13 at 113,368,776 bp
  • T to C, chromosome 14 at 26,772,644 bp
  • T to C, chromosome 15 at 31,469,532 bp
  • T to C, chromosome 15 at 99,321,132 bp
  • T to C, chromosome 16 at 56,690,988 bp
  • A to G, chromosome 16 at 97,451,588 bp
  • A to G, chromosome 17 at 19,811,979 bp
  • A to T, chromosome 17 at 26,083,053 bp
  • A to G, chromosome 17 at 58,199,017 bp
  • A to T, chromosome 17 at 84,557,392 bp
  • C to T, chromosome 18 at 60,916,986 bp
  • C to T, chromosome 18 at 60,960,007 bp
  • T to C, chromosome 18 at 77,339,500 bp
  • T to C, chromosome 18 at 80,130,990 bp
  • T to A, chromosome 19 at 4,880,374 bp
  • G to A, chromosome 19 at 10,539,629 bp
  • G to A, chromosome 19 at 10,940,428 bp
  • T to C, chromosome 19 at 13,827,686 bp
  • T to C, chromosome Y at 926,999 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain GQC Summary
The MMRRC Centers have developed a Strain GQC pipeline using MiniMUGA array genotyping to provide additional information on strain backgrounds for MMRRC strains. For more information on whether data may be available, or to request genotyping for a strain of interest, please contact MMRRC_GeneticQC@med.unc.edu. Older strains may not have this information available.
Suggested Control Mice
Littermates of all relevant genotypes.
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0003 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.


Disclaimer: If MMRRC Strain Genetic Quality Control (GQC; based on MiniMUGA genotyping and analysis) has been completed for this strain, the information might differ from the genetic background information provided by the submitter. MiniMUGA genetic analysis is done on a strain’s tissue samples taken when archived by or ordered from the assigned MMRRC Center.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu.
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $)
*Shipping & Handling not included*
Units Notes
038299-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality, nor genotype, of this material. Our expertise in reviving mice from various qualities of frozen sperm and embryos, using methods like IVF and ICSI, gives us confidence in successful recoveries in most cases. However, due to the uncertain quality of these samples, we'll limit revival attempts to two per order. Additional attempts are available for a fee, on top of standard charges, if requested. It's important to note that some strains may lack the expected mutation, so we can't assure successful order fulfillment until we attempt to revive the strain.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.