Strain Name:
C57BL/6J-MtgxR0005Btlr/Mmmh
Stock Number:
038301-MU
Citation ID:
RRID:MMRRC_038301-MU
Other Names:
R0005 (G1), C57BL/6J-MtgxR0005Btlr
Major Collection:

Gene Information

Vsx2
Name: visual system homeobox 2
Synonyms: Hox-10, Chx10, Hox10
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 12
NCBI: 12677
HGNC: HGNC:1975
Homologene: 7266
Cemip2
Name: cell migration inducing hyaluronidase 2
Synonyms: 3110012M15Rik, Tmem2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 19
NCBI: 83921
VEGA: 19
Homologene: 75008
Camsap3
Name: calmodulin regulated spectrin-associated protein family, member 3
Synonyms: Nezha, 2310057J16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 69697
Homologene: 18966
Kif1b
Name: kinesin family member 1B
Synonyms: KIF1Bp204, KIF1Bp130, A530096N05Rik, D4Mil1e, N-3 kinesin, Kif1b alpha, Kif1b beta
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 16561
Homologene: 99835
Wdr48
Name: WD repeat domain 48
Synonyms: Uaf1, 8430408H12Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 67561
VEGA: 9
Homologene: 10830
Atxn2l
Name: ataxin 2-like
Synonyms: A2D, A2RP, A2LG, A2lp
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233871
Homologene: 16513
Plekhg5
Name: pleckstrin homology domain containing, family G (with RhoGef domain) member 5
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 269608
Homologene: 10768
Map3k1
Name: mitogen-activated protein kinase kinase kinase 1
Synonyms: Mekk, MEKK1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 13
NCBI: 26401
HGNC: HGNC:6848
Homologene: 8056
Zfp335
Name: zinc finger protein 335
Synonyms: 1810045J01Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 329559
Homologene: 11129
Myadm
Name: myeloid-associated differentiation marker
Synonyms: D7Wsu62e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 50918
HGNC: HGNC:7544
Homologene: 9668
Muc6
Name: mucin 6, gastric
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 353328
HGNC: HGNC:7517
Homologene: 18768
Fat3
Name: FAT atypical cadherin 3
Synonyms: LOC234973, 9430076A06Rik, D430038H04Rik, LOC382129
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 270120
VEGA: 9
Homologene: 82252
Col11a2
Name: collagen, type XI, alpha 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 12815
HGNC: HGNC:2187
Homologene: 22547
Plekhh2
Name: pleckstrin homology domain containing, family H (with MyTH4 domain) member 2
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 213556
VEGA: 17
Homologene: 35317
Lamc3
Name: laminin gamma 3
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 2
NCBI: 23928
HGNC: HGNC:6494
Homologene: 21222
Col27a1
Name: collagen, type XXVII, alpha 1
Synonyms: 5730512J02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 373864
Homologene: 69400
Ppih
Name: peptidyl prolyl isomerase H
Synonyms: cyclophilin H, 2010111B15Rik, 4833408F11Rik, rotamase H, 1100001J08Rik, CYP-20, D4Wsu43e
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 66101
Homologene: 38172
Spocd1
Name: SPOC domain containing 1
Synonyms: OTTMUSG00000009522
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 622480
Homologene: 83439
Pramef12
Name: PRAME family member 12
Synonyms: 4930569K13Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 4
NCBI: 77632
Homologene: 135701
Gabra2
Name: gamma-aminobutyric acid (GABA) A receptor, subunit alpha 2
Synonyms: Gabra-2, C630048P16Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 14395
HGNC: HGNC:4076
Homologene: 20217
Map11
Name: microtubule associated protein 11
Synonyms: BC037034
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 5
NCBI: 231807
Homologene: 10106
Plcz1
Name: phospholipase C, zeta 1
Synonyms: 1700041H07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 6
NCBI: 114875
Homologene: 23815
Nlrp9a
Name: NLR family, pyrin domain containing 9A
Synonyms: Nalp-theta, D7Ertd565e, Nalp9a
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 233001
Homologene: 116072
Mag
Name: myelin-associated glycoprotein
Synonyms: siglec-4a, Gma
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 7
NCBI: 17136
HGNC: HGNC:6783
Homologene: 1771
Mylk3
Name: myosin light chain kinase 3
Synonyms: D830007F02Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 8
NCBI: 213435
Homologene: 35278
Olfr974
Name: olfactory receptor 974
Synonyms: GA_x6K02T2PVTD-33640290-33641222, MOR171-1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 9
NCBI: 259111
VEGA: 9
Homologene: 133680
Hivep2
Name: human immunodeficiency virus type I enhancer binding protein 2
Synonyms: Schnurri-2, Gm20114, Shn-2, MIBP1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 15273
HGNC: HGNC:4921
Homologene: 4900
Rtn4ip1
Name: reticulon 4 interacting protein 1
Synonyms: D10Ertd690e, NIMP
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 10
NCBI: 170728
Homologene: 13105
Tmed4
Name: transmembrane p24 trafficking protein 4
Synonyms: 1110014L17Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 103694
Homologene: 5308
Stxbp4
Name: syntaxin binding protein 4
Synonyms: 6030470M02Rik, Synip
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 11
NCBI: 20913
Homologene: 7963
Slc35f4
Name: solute carrier family 35, member F4
Synonyms: 4930550L21Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 14
NCBI: 75288
Homologene: 45939
Cpsf1
Name: cleavage and polyadenylation specific factor 1
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 15
NCBI: 94230
VEGA: 15
HGNC: HGNC:2324
Homologene: 40865
2410017I17Rik
Name: RIKEN cDNA 2410017I17 gene
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 675325
Enpp4
Name: ectonucleotide pyrophosphatase/phosphodiesterase 4
Synonyms: LOC224794, 4933413N07Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 224794
HGNC: HGNC:3359
Homologene: 28264
Tnfsf9
Name: tumor necrosis factor (ligand) superfamily, member 9
Synonyms: 4-1BBL, Ly63l, 4-1BB ligand, 4-1BB-L, Cd137l
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 17
NCBI: 21950
VEGA: 17
Homologene: 137221
Mapre2
Name: microtubule-associated protein, RP/EB family, member 2
Synonyms: RP1, D18Abb1e, EB2, C820009F03Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 212307
HGNC: HGNC:6891
Homologene: 134539
Zfp608
Name: zinc finger protein 608
Synonyms: 4932417D18Rik
Type: Gene
Species: Mus musculus (mouse)
Chromosome: 18
NCBI: 269023
VEGA: 18
Homologene: 18485
Genetic Alterations
ENU-induced transitions at the following base pair locations (GRCm38):
  • A to G, chromosome 2 at 31,922,428 bp
  • T to A, chromosome 2 at 164,909,302 bp
  • A to G, chromosome 4 at 63,225,400 bp
  • A to G, chromosome 4 at 119,318,601 bp
  • G to T, chromosome 4 at 129,956,778 bp
  • G to T, chromosome 4 at 144,395,853 bp
  • A to T, chromosome 4 at 149,181,927 bp
  • TCCCCC to TCC, chromosome 4 at 152,112,651 bp
  • C to T, chromosome 5 at 70,973,436 bp
  • T to C, chromosome 5 at 138,262,654 bp
  • T to A, chromosome 6 at 140,040,564 bp
  • C to T, chromosome 7 at 3,297,564 bp
  • T to C, chromosome 7 at 26,573,788 bp
  • T to A, chromosome 7 at 30,908,354 bp
  • A to G, chromosome 7 at 126,498,274 bp
  • G to T, chromosome 7 at 141,638,772 bp
  • T to A, chromosome 8 at 3,604,288 bp
  • A to T, chromosome 8 at 85,327,203 bp
  • T to A, chromosome 9 at 15,962,866 bp
  • A to G, chromosome 9 at 39,942,956 bp
  • T to A, chromosome 9 at 119,909,434 bp
  • A to G, chromosome 10 at 14,128,749 bp
  • A to T, chromosome 10 at 43,932,478 bp
  • C to T, chromosome 11 at 6,271,781 bp
  • C to T, chromosome 11 at 90,548,917 bp
  • C to A, chromosome 12 at 84,570,241 bp
  • A to G, chromosome 13 at 111,755,704 bp
  • G to A, chromosome 14 at 49,322,486 bp
  • A to G, chromosome 15 at 76,600,680 bp
  • C to T, chromosome 17 at 34,062,879 bp
  • G to T, chromosome 17 at 36,162,192 bp
  • T to C, chromosome 17 at 44,102,175 bp
  • T to C, chromosome 17 at 57,107,236 bp
  • A to C, chromosome 17 at 84,586,433 bp
  • G to A, chromosome 18 at 23,853,693 bp
  • G to A, chromosome 18 at 54,895,520 bp
  • A to G, chromosome 19 at 21,812,220 bp
Genotype Determination
Phenotype
G1 male heterozygous mice are viable and fertile. The G1 mice were not characterized prior to archiving.
Strain Development
C57BL/6J mice were injected with ENU, resulting in G0 mice that were bred back to C57BL/6J. Sperm was archived from G1 male mice that produced viable G2 mice.
Suggested Control Mice
Wild-type littermates or C57BL/6J mice
Donor
Bruce Beutler, M.D., University of Texas Southwestern Medical Center.
Primary Reference
MUTAGENETIX record for R0005 (G1). B. Beutler and colleagues, Center for the Genetics of Host Defense, UT Southwestern, Dallas, TX.

Colony and Husbandry Information

Colony Surveillance Program and Current Health Reports

Mice recovered from a cryo-archive will have health surveillance performed on recipient females. Health reports will be provided prior to shipment. If you require additional health status information, please email mmrrc@missouri.edu .
Coat Color
Black
MMRRC Breeding System
Random intra-strain mating
Breeding Scheme(s)
When maintaining a live colony, heterozygous mice may be bred together, bred with wild-type siblings, or bred with C57BL/6J inbred mice.
Overall Breeding Performance
Undetermined; during development, mating was done with heterozygous males; viability, fertility and other breeding statistics for heterozygous females and homozygotes are unverified, but presumably would be similar to the C57BL/6J background strain
Average litter size
Undetermined
Recommended wean age
3 weeks

Order Request Information

Limited quantities of breeder mice (recovered litter) are available from a cryoarchive; recovered litter usually available to ship in 3 to 4 months.

Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Distribution of this strain requires submission of the MMRRC Conditions of Use (COU). A link to the COU web form will be provided via email after an order has been placed; the form should be completed then or the email forwarded to your institutional official for completion.

The donor or their institution limits the distribution to non-profit institutions only.

Additional charges may apply for any special requests. Shipping costs are in addition to the basic distribution/resuscitation fees. Information on shipping costs and any additional charges will be provided by the supplying MMRRC facility.

Click button to Request this one strain. (Use the MMRRC Catalog Search to request more than one strain.)
MMRRC Item # Description Distribution Fee / Unit (US $) Units Notes
038301-MU-RESUS Litter recovered from cryo-archive $5,475.00 / Non-Profit Litter Recovered litter4; additional fees for any special requests.
Cryopreserved material may be available upon request, please inquire to mmrrc@missouri.edu for more information.

Some MMRRC strains were submitted by the donor as cryopreserved germplasm, and because these strains were not cryopreserved by the MMRRC, we have not assessed the quality of this material. We have extensive experience with recovery of mice from cryopreserved sperm and embryos of varying quality, including using techniques such as IVF and ICSI, and are confident that we will have success in most, if not all, cases. However, because of the unknown quality of these frozen samples, we will restrict recovery efforts to two attempts for each order. Subsequent attempts can be performed on a fee-for-service basis, in addition to the standard fees, upon request.

1 The distribution fee covers the expense of rederiving mice from a live mouse; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

2 An aliquot contains a sufficient number of embryos (in one or more vials or straws and based on the transfer success rate of the MMRRC facility) to transfer into one to three recipients. The MMRRC makes no guarantee concerning embryo transfer success experienced in the recipient investigator's laboratory. Neither gender nor genotype ratios are guaranteed.

3 An aliquot is one straw or vial with sufficient sperm to recover at least one litter of mice, as per provided protocols, when performed at the MMRRC facility. The MMRRC makes no guarantee concerning the success of these procedures when performed outside the MMRRC facilities.

4 The distribution fee covers the expense of resuscitating mice from the cryo-archive; you will receive the resulting litter. The litter will contain at minimum one mutant carrier; the actual number of animals and the gender and genotype ratios will vary. (Typically, multiple breeder pairs can be established from the recovered litter.) Prior to shipment, the MMRRC will provide information about the animals recovered. If you anticipate or find that you need to request specific genotypes, genders or quantities of mice in excess of what is likely from a resuscitated litter, you may discuss available options and pricing with the supplying MMRRC facility.

To request material from the MMRRC: Please fill out our on-line request form (accessible from the catalog search results page, or click the Request this Strain button in the fees section). If you have questions or need assistance completing this form, you may call Customer Service at (800) 910-2291 (in USA or Canada) or (530) 757-5710 (international calls). Before you call, please have with you: the MMRRC item number, quantity needed, Bill-to and Ship-to contact information.